Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,897,086 T→C C105R (TGT→CGT)  yieH → phosphoenolpyruvate and 6‑phosphogluconate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,897,0860TC100.0% 84.6 / NA 24C105R (TGT→CGT) yieHphosphoenolpyruvate and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/12);  total (12/12)

CTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGC  >  NC_000913/3896951‑3897210
                                                                                                                                       |                                                                                                                            
cTTGAACATGGTGTTACGTTAGCGCAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgcgtg                                                                                                                           <  2:279530/139‑1 (MQ=255)
cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgcgtg                                                                                                                           <  1:278485/139‑1 (MQ=255)
   gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTgg                                                                                                                        >  2:385633/1‑139 (MQ=255)
              tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGc                                                                                                             <  2:357146/139‑1 (MQ=255)
              tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGc                                                                                                             <  2:196500/139‑1 (MQ=255)
                 gTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCa                                                                                                          <  2:364009/139‑1 (MQ=255)
                   tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCaaa                                                                                                        >  1:77147/1‑139 (MQ=255)
                     gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATa                                                                                                      >  1:393453/1‑139 (MQ=255)
                     gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATa                                                                                                      >  2:279361/1‑139 (MQ=255)
                                aGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAACAAAATgcag                                                                                           <  2:432030/139‑1 (MQ=255)
                                              aCCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCaa                                                                             <  1:79567/139‑1 (MQ=255)
                                               ccGTGCAGAAGTCGCTCGGCTGTACGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAg                                                                            <  2:170540/139‑1 (MQ=255)
                                                    cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGaa                                                                       >  2:17393/1‑139 (MQ=255)
                                                            gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgcgtg                                                                                                                           <  2:62193/79‑1 (MQ=255)
                                                            gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgcgtg                                                                                                                           >  1:62193/1‑79 (MQ=255)
                                                            gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGc                                                               >  2:178773/1‑139 (MQ=255)
                                                                 gCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCaaa                                                                                                        >  1:468435/1‑93 (MQ=255)
                                                                 gCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCaaa                                                                                                        <  2:468435/93‑1 (MQ=255)
                                                                                        ccATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg                                   <  1:178773/139‑1 (MQ=255)
                                                                                                     tGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAg                      <  2:393453/139‑1 (MQ=255)
                                                                                                        aGCGCTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAgcgc                   >  1:155560/1‑139 (MQ=255)
                                                                                                          cgcTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTg                 <  1:317312/139‑1 (MQ=255)
                                                                                                            cTCCTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGa               >  1:205787/1‑139 (MQ=255)
                                                                                                               cTGTCAGCGATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAAAGAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTCCTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGc            <  2:318018/139‑1 (MQ=255)
                                                                                                                       gATCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCg    >  1:263979/1‑139 (MQ=255)
                                                                                                                         tCACTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGc  >  2:471355/1‑139 (MQ=255)
                                                                                                                            cTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCact                                                            <  1:368407/78‑1 (MQ=255)
                                                                                                                            cTGCGCCAATGCGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCact                                                            >  2:368407/1‑78 (MQ=255)
                                                                                                                                       |                                                                                                                            
CTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCTACGATATTCAGCGCTGGAAGCCAGACCCGGC  >  NC_000913/3896951‑3897210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: