Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,926,801 A→G V68A (GTA→GCA)  asnC ← transcriptional activator of asnA, autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,926,8010AG100.0% 92.6 / NA 25V68A (GTA→GCA) asnCtranscriptional activator of asnA, autorepressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/13);  total (12/13)

GATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAG  >  NC_000913/3926683‑3926922
                                                                                                                      |                                                                                                                         
gATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTc                                                                                                       <  1:256872/139‑1 (MQ=255)
 aTGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCg                                                                                                      >  2:260360/1‑139 (MQ=255)
   gCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCggg                                                                                                    <  2:386843/139‑1 (MQ=255)
    cTGTAGTGGCCGGTTGTGTAATAGACTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGc                                                                                                   >  2:468757/1‑139 (MQ=255)
                 ttGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcg                                                                                      <  1:260360/139‑1 (MQ=255)
                 ttGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcg                                                                                      <  2:445822/139‑1 (MQ=255)
                 ttGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcg                                                                                      <  2:276888/139‑1 (MQ=255)
                      tAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGccc                                                                                 <  2:461912/139‑1 (MQ=255)
                           ggCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcg                                                                                      <  1:388242/129‑1 (MQ=255)
                           ggCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcg                                                                                      >  2:388242/1‑129 (MQ=255)
                              ttCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATg                                                                         >  2:462413/1‑139 (MQ=255)
                                cAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCggg                                                                                                    <  2:10301/110‑1 (MQ=255)
                                cAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCggg                                                                                                    >  1:10301/1‑110 (MQ=255)
                                                         cTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACt                                              <  1:468757/139‑1 (MQ=255)
                                                         cTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACt                                              <  2:40780/139‑1 (MQ=255)
                                                                                 tttGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGccc                                                                                 <  1:429486/80‑1 (MQ=255)
                                                                                 tttGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGccc                                                                                 >  2:429486/1‑80 (MQ=255)
                                                                                                     tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc                                                                                   <  1:454580/58‑1 (MQ=255)
                                                                                                     tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc                                                                                   >  2:454580/1‑58 (MQ=255)
                                                                                                     tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCa                                                         <  2:198026/84‑1 (MQ=255)
                                                                                                     tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCa                                                         >  1:198026/1‑84 (MQ=255)
                                                                                                     tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg  >  2:366535/1‑139 (MQ=255)
                                                                                                     tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg  >  1:400052/1‑139 (MQ=255)
                                                                                                     tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg  >  1:182091/1‑139 (MQ=255)
                                                                                                     tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg  >  1:162713/1‑139 (MQ=255)
                                                                                                                      |                                                                                                                         
GATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAG  >  NC_000913/3926683‑3926922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: