Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 427,630 (T)5→4 intergenic (+11/‑17) yajC → / → secD SecYEG protein translocase auxillary subunit/SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913427,6260T.100.0% 157.8 / NA 39intergenic (+7/‑21)yajC/secDSecYEG protein translocase auxillary subunit/SecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (20/19);  total (20/19)

TGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGAT  >  NC_000913/427489‑427756
                                                                                                                                         |                                                                                                                                  
tggttggtCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAAtt                                                                                                                                   >  2:51730/1‑139 (MQ=255)
       tCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTa                                                                                                                           >  2:784623/1‑139 (MQ=255)
       tCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTa                                                                                                                           >  1:225284/1‑139 (MQ=255)
                cAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTg                                                                                                                  <  2:1093458/139‑1 (MQ=255)
                        cGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTa                                                                                                          <  1:108358/139‑1 (MQ=255)
                          gAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTaaa                                                                                                        <  1:61422/139‑1 (MQ=255)
                              aCGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGt                                                                                                    >  1:679141/1‑139 (MQ=255)
                               cGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGtt                                                                                                   >  1:176196/1‑139 (MQ=255)
                                  cTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATc                                                                                                <  1:637945/139‑1 (MQ=255)
                                  cTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATc                                                                                                <  2:679335/139‑1 (MQ=255)
                                    aCATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCt                                                                                              <  2:176196/139‑1 (MQ=255)
                                    aCATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCt                                                                                              <  1:163860/139‑1 (MQ=255)
                                          cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTgg                                                                                        >  2:719432/1‑139 (MQ=255)
                                              cgcgCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGt                                                                                    <  1:784623/139‑1 (MQ=255)
                                                 gcTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACg                                                                                 <  2:566797/139‑1 (MQ=255)
                                                    gAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCa                                                                              >  1:188761/1‑139 (MQ=255)
                                                                       gtTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGt                                                           >  2:299158/1‑139 (MQ=255)
                                                                        ttattaAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGc                                                                                                                 <  1:1041359/84‑1 (MQ=255)
                                                                        ttattaAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGc                                                                                                                 >  2:1041359/1‑84 (MQ=255)
                                                                        ttattaAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTa                                                                                                          >  1:260553/1‑91 (MQ=255)
                                                                        ttattaAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTa                                                                                                          <  2:260553/91‑1 (MQ=255)
                                                                          attaAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTc                                                                               >  1:1094734/1‑116 (MQ=255)
                                                                          attaAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTc                                                                               <  2:1094734/116‑1 (MQ=255)
                                                                            taAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTa                                                                                                          >  1:280470/1‑87 (MQ=255)
                                                                            taAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTa                                                                                                          <  2:280470/87‑1 (MQ=255)
                                                                                    aCTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTctgctg                                              >  2:861167/1‑139 (MQ=255)
                                                                                    aCTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTATCGTGGTGATTGTCATCGGTCTGCTGt                                             >  1:1061978/1‑139 (MQ=255)
                                                                                                ccGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACg                                                                                 <  2:315889/92‑1 (MQ=255)
                                                                                                ccGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACg                                                                                 >  1:315889/1‑92 (MQ=255)
                                                                                                   tCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTa                                                                                                                           <  2:171752/47‑1 (MQ=255)
                                                                                                   tCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTa                                                                                                                           >  1:171752/1‑47 (MQ=255)
                                                                                                   tCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACAGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAAc                               >  1:688185/1‑139 (MQ=255)
                                                                                                                 aCCATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGa                 <  2:688185/139‑1 (MQ=255)
                                                                                                                  ccATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTg                                                                                                                  >  2:881909/1‑41 (MQ=255)
                                                                                                                  ccATGAAGGCGCTGTAATTAAAATTTTCCCTAAGGGAATTg                                                                                                                  <  1:881909/41‑1 (MQ=255)
                                                                                                                           cgcTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGtt       <  1:665285/139‑1 (MQ=255)
                                                                                                                            gcTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGaa                                                                                      >  2:441480/1‑59 (MQ=255)
                                                                                                                            gcTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGaa                                                                                      <  1:441480/59‑1 (MQ=255)
                                                                                                                             cTGTAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCa     >  1:825514/1‑139 (MQ=255)
                                                                                                                                tAATTAAAATTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGAt  >  1:860076/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                  
TGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGAT  >  NC_000913/427489‑427756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: