Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 380,022 (G)10→11 intergenic (‑141/+47) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0121.G89.6% 153.3 / 10.0 49intergenic (‑131/+57)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base . (2/3);  new base G (28/15);  total (31/18)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACAC  >  NC_000913/379902‑380140
                                                                                                                |                                                                                                                                 
agacagataCTATAGGGGGGTATTATATATGTCAATGCAAACCCCCCTATAGTATAAAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                       <  1:988114/133‑1 (MQ=255)
                 gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGGAGGGAAGGCCGCCCCGGAGAgcggg                                                                                      >  2:13052/1‑139 (MQ=255)
                 gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAtctgtct                                                                                      >  2:988114/1‑133 (MQ=255)
                           tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGTCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                                                            >  2:72330/1‑139 (MQ=255)
                              gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                                                         >  1:622169/1‑139 (MQ=255)
                                 aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa                                                                       >  2:913201/1‑139 (MQ=255)
                                 aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg                                                                      >  1:912643/1‑139 (MQ=255)
                                   tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATGGGCGGGGAAGGCCGCCCCGGATAGGGGGGGGCAGAAGGAATAAGaa                                                                    >  1:941987/1‑139 (MQ=255)
                                   tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGCGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa                                                                    >  2:1074413/1‑139 (MQ=255)
                                        aCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTcc                                                               >  1:661729/1‑139 (MQ=255)
                                                  aGTATAAAAAAATGGAGAAAAAAATTTATCCGGGGAAAGGGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACg                                                     <  2:941987/139‑1 (MQ=255)
                                                      tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGcg                                                 >  1:951687/1‑138 (MQ=255)
                                                      tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                 >  2:296072/1‑139 (MQ=255)
                                                      tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAAACGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                 >  1:420242/1‑139 (MQ=255)
                                                           cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACACAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAGGGCCGCCCCGGATAGCGGGCGGGGGAAGGAATCAGAATTCCCAGGTCAGACGGGCGGCAAg                                            >  1:464170/1‑139 (MQ=255)
                                                           cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGCAAATAGGCCGGGGAGGGCGCACCGGAAAGCGGGCGGCAGAAGGGAACGAAAGTTCCAGGTCAAACgggagggca                                             >  1:745953/1‑133 (MQ=255)
                                                           cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGccc                                                                                                 >  1:205687/1‑86 (MQ=255)
                                                           cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGGAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg                                            >  2:262478/1‑139 (MQ=255)
                                                           cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg                                            >  1:719278/1‑139 (MQ=255)
                                                           cacTGGAGAATAAAATTTATCCGGGGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGccc                                                                                                 <  2:205687/86‑1 (MQ=255)
                                                              tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg                                                                                       <  2:919024/94‑1 (MQ=255)
                                                              tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg                                                                                       >  1:919024/1‑94 (MQ=255)
                                                              tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                       >  1:589143/1‑77 (MQ=255)
                                                              tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                       <  2:589143/77‑1 (MQ=255)
                                                              tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg                                         >  2:860518/1‑139 (MQ=255)
                                                                gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc                                        >  2:983505/1‑139 (MQ=255)
                                                                gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                       >  2:1008518/1‑139 (MQ=255)
                                                                gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                       <  2:661729/139‑1 (MQ=255)
                                                                gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                       <  1:860518/139‑1 (MQ=255)
                                                                gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGAAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTgaa                                       >  1:754932/1‑137 (MQ=255)
                                                                gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg                                      >  2:370736/1‑139 (MQ=255)
                                                                gagaATAAAATTTATCCGGTGAATGGGGGGGCAACAAAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                       <  1:262478/139‑1 (MQ=255)
                                                                 ggaAAAAAATTTTTCCGGGGAATGTGGGGGGAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                       <  1:185165/138‑1 (MQ=255)
                                                                 agaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa                                     <  1:370736/139‑1 (MQ=255)
                                                                   aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAATGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGGTAGCGGACGGCAGAAGGGATGAGAATTTCCAGGTCAGACGGGCTGCAAGTTGAAGa                                     >  1:819603/1‑139 (MQ=255)
                                                                     tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAcc                                   >  1:681499/1‑139 (MQ=255)
                                                                             aTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCGGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaaac                           >  2:185165/1‑138 (MQ=255)
                                                                               ccGGTGAATGTGGTCGGAAAACAAAGGGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa                                                                              <  1:545354/85‑1 (MQ=255)
                                                                               ccGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa                                                                              >  2:545354/1‑85 (MQ=255)
                                                                                  gggAAGGTGGTCGGAAAACAAAGAGGAGAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCgg                     <  2:464170/137‑1 (MQ=255)
                                                                                  gTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcatc                       >  2:49681/1‑136 (MQ=255)
                                                                                  gTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcatc                       <  1:49681/136‑1 (MQ=255)
                                                                                   tGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATGGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt                    >  1:27218/1‑139 (MQ=255)
                                                                                     aaTGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTg                  <  1:1074413/139‑1 (MQ=255)
                                                                                       tgtgGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTggg                <  2:622169/139‑1 (MQ=255)
                                                                                            tCGGAAATCAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa                                              >  1:772784/1‑104 (MQ=255)
                                                                                            tCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa                                              <  2:772784/104‑1 (MQ=255)
                                                                                               gAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGa                                                                           <  2:556873/73‑1 (MQ=255)
                                                                                               gAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGa                                                                           >  1:556873/1‑73 (MQ=255)
                                                                                                 aaaCAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc       <  2:681499/139‑1 (MQ=255)
                                                                                                 aaaCAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc       <  1:913201/139‑1 (MQ=255)
                                                                                                     aaaGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCacac  <  2:719278/139‑1 (MQ=255)
                                                                                                            aaa‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                               >  2:674360/1‑86 (MQ=255)
                                                                                                            aaa‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                               <  1:674360/86‑1 (MQ=255)
                                                                                                                |                                                                                                                                 
TGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACAC  >  NC_000913/379902‑380140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: