Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,271,600	(C)5→6	coding (171/1146 nt)	garK ←	glycerate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,271,595	1	.	C	100.0%	127.2 / NA	34	A59G (GCT→GGT)	garK	glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (18/16);  total (18/16)

TCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAG‑CCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGG  >  NC_000913/3271457‑3271724                                                                                                                                            |                                                                                                                                  tCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAg                                                                                                                                    <  2:446383/139‑1 (MQ=255)           ccGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAG‑cccccctggg                                                                                                                         <  1:979972/139‑6 (MQ=255)                         tCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAatcatc                                                                                                            <  1:895203/139‑1 (MQ=255)                           cAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGc                                                                                                          >  1:198711/1‑139 (MQ=255)                            aGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCt                                                                                                         <  1:325781/139‑1 (MQ=255)                             gCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCtt                                                                                                        >  1:508220/1‑139 (MQ=255)                             gCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCtt                                                                                                        >  1:541296/1‑139 (MQ=255)                             gCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCtt                                                                                                        >  2:871609/1‑139 (MQ=255)                               ccACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTcc                                                                                                      >  2:270905/1‑139 (MQ=255)                                                         cgcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAAc                                                                            <  2:508220/139‑1 (MQ=255)                                                            ggTTTGGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCgg                                                                         <  1:1063317/139‑1 (MQ=255)                                                                   ccATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGaa                                                                  <  1:871609/139‑1 (MQ=255)                                                                                      aaCTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGATGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGaa                                               >  1:515964/1‑139 (MQ=255)                                                                                           gCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAAtt                                          >  1:856417/1‑139 (MQ=255)                                                                                           gCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAAtt                                          >  2:499757/1‑139 (MQ=255)                                                                                           gCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAAtt                                          >  2:360825/1‑139 (MQ=255)                                                                                            cATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGc                                                                                         <  1:211698/91‑1 (MQ=255)                                                                                            cATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGc                                                                                         >  2:211698/1‑91 (MQ=255)                                                                                              ttCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTcc                                       >  2:89248/1‑139 (MQ=255)                                                                                                  cTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGaa                                   <  1:556929/139‑1 (MQ=255)                                                                                                  cTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGaa                                   <  1:89248/139‑1 (MQ=255)                                                                                                  cTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGaa                                   <  1:499757/139‑1 (MQ=255)                                                                                                    ttCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAAt                                 <  1:558697/139‑1 (MQ=255)                                                                                                        cGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCtt                             <  2:238802/139‑1 (MQ=255)                                                                                                                         aCCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTAt                      >  1:568417/1‑129 (MQ=255)                                                                                                                         aCCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTAt                      <  2:568417/129‑1 (MQ=255)                                                                                                                             aGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCt        >  1:1093279/1‑139 (MQ=255)                                                                                                                              ggCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGaa                                                                       >  1:903053/1‑75 (MQ=255)                                                                                                                              ggCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGaa                                                                       <  2:903053/75‑1 (MQ=255)                                                                                                                              ggCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCC‑CCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCg      >  1:551812/1‑139 (MQ=255)                                                                                                                               gCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTc                                                                                                       <  2:169654/42‑1 (MQ=255)                                                                                                                               gCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTc                                                                                                       >  1:169654/1‑42 (MQ=255)                                                                                                                               gCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCg                                                                          <  1:812553/71‑1 (MQ=255)                                                                                                                               gCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCg                                                                          >  2:812553/1‑71 (MQ=255)                                                                                                                               gCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCg      >  2:1066148/1‑139 (MQ=255)                                                                                                                                   gACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTgg  <  2:1093279/139‑1 (MQ=255)                                                                                                                                            |                                                                                                                                  TCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAG‑CCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGG  >  NC_000913/3271457‑3271724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑