Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,686	C→T	V92I (GTC→ATC)	yaaJ ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,686	0	C	T	100.0%	106.8 / NA	31	V92I (GTC→ATC)	yaaJ	putative transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

TACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTT  >  NC_000913/7565‑7816                                                                                                                          |                                                                                                                                   taCCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACcgg                                                                                                                   <  1:1016120/139‑1 (MQ=255)       gCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTaa                                                                                                             >  1:609630/1‑139 (MQ=255)          ggTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAg                                                                                                          >  2:1171485/1‑139 (MQ=255)                                    gTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCg                                                                                <  1:930798/139‑1 (MQ=255)                                     tCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATGCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGACAGCGCAACGCCGGCCAGGTTGCCGc                                                                               >  1:750003/1‑139 (MQ=255)                                     tCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGt                                                                                     <  2:341382/133‑1 (MQ=255)                                     tCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGt                                                                                     >  1:341382/1‑133 (MQ=255)                                             tttATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAcg                                                                       >  1:625603/1‑139 (MQ=255)                                                     gTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACCCCGGCCAGGTTGCCGCTACCCACGCGcgccgc                                                               <  2:520940/139‑1 (MQ=255)                                                       tgtgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCaa                                                             >  1:402747/1‑139 (MQ=255)                                                         tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACcgg                                                                                                                   <  1:360581/83‑1 (MQ=255)                                                         tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACcgg                                                                                                                   >  2:360581/1‑83 (MQ=255)                                                         tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAgcg                                                                                                    <  1:82952/98‑1 (MQ=255)                                                         tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAgcg                                                                                                    >  2:82952/1‑98 (MQ=255)                                                         tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAcgcg                                                                     >  2:650500/1‑129 (MQ=255)                                                         tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAcgcg                                                                     <  1:650500/129‑1 (MQ=255)                                                         tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGa                                                           >  1:946110/1‑139 (MQ=255)                                                         tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGa                                                           >  2:571163/1‑139 (MQ=255)                                                         tgCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATCGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGcgccg                                                                <  2:734933/134‑1 (MQ=255)                                                         agCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGcgccg                                                                >  1:734933/2‑134 (MQ=255)                                                              gagaACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGt                                                      >  2:897390/1‑139 (MQ=255)                                                                             aCGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGaaa                                       >  1:1143865/1‑139 (MQ=255)                                                                             aCGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAACCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGaaa                                       <  2:713810/139‑1 (MQ=255)                                                                              cGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAAt                                      <  2:946110/139‑1 (MQ=255)                                                                                           ccGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGc                         <  2:625603/139‑1 (MQ=255)                                                                                            cGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGcc                        <  2:609630/139‑1 (MQ=255)                                                                                            cGATAAACGCGGCAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGcc                        <  2:402747/139‑1 (MQ=255)                                                                                                        cAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGa                                                           <  2:49030/92‑1 (MQ=255)                                                                                                        cAACCCACATCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGa                                                           >  1:49030/1‑92 (MQ=255)                                                                                                              acaTCCAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCt      >  1:690895/1‑139 (MQ=255)                                                                                                                  ccAAAAGATGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTAttt  >  2:561268/1‑139 (MQ=255)                                                                                                                          |                                                                                                                                   TACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTT  >  NC_000913/7565‑7816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑