BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	992,344	T→C	Y575H (TAC→CAC)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	992,344	0	T	C	100.0%	139.7 / NA	37	Y575H (TAC→CAC)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/16); total (21/16)

TCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCAGAAATCCTGACGC  >  NC_000913/992228‑992476

tctcTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCCAGCTGAACGTcgcgcg                                                                                                                >  2:801888/1‑139 (MQ=255)

tctcTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTcgcgcg                                                                                                                >  2:383562/1‑139 (MQ=255)

  tctGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGtc                                                                                                              <  2:85136/139‑1 (MQ=255)

            tGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAgg                                                                                                    >  1:815135/1‑139 (MQ=255)

             gAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGt                                                                                                   <  2:334482/139‑1 (MQ=255)

                                     tCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCAt                                                                           <  1:801888/139‑1 (MQ=255)

                                               aCCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCg                                                                                             <  1:25784/111‑1 (MQ=255)

                                               aCCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCg                                                                                             >  2:25784/1‑111 (MQ=255)

                                               aCCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATg                                                                 >  1:973547/1‑139 (MQ=255)

                                                 cTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCcaca                                                                                                                                   <  2:698450/71‑1 (MQ=255)

                                                 cTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCcaca                                                                                                                                   >  1:698450/1‑71 (MQ=255)

                                                 cTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCt                                                               >  1:1045478/1‑139 (MQ=255)

                                                 cTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCt                                                               >  1:158080/1‑139 (MQ=255)

                                                 cTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGCGGCTGATGCt                                                               >  1:563262/1‑139 (MQ=255)

                                                   tCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCttt                                                             <  1:383562/139‑1 (MQ=255)

                                                         tGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTcg                                                                                                                    <  2:190067/78‑1 (MQ=255)

                                                         tGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTcg                                                                                                                    >  1:190067/1‑78 (MQ=255)

                                                           cGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCGGCGCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCcgcgc                                                       >  1:467538/1‑139 (MQ=255)

                                                            gTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGt                                                    <  2:973547/139‑1 (MQ=255)

                                                             tCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTa                                                   >  1:1073368/1‑139 (MQ=255)

                                                              cATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTcgcg                                                                                                                  <  1:622592/75‑1 (MQ=255)

                                                              cATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTcgcg                                                                                                                  >  2:622592/1‑75 (MQ=255)

                                                              cATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTAc                                                  >  2:744493/1‑139 (MQ=255)

                                                               aTGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGtc                                                                                                              <  2:163618/78‑1 (MQ=255)

                                                               aTGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGtc                                                                                                              >  1:163618/1‑78 (MQ=255)

                                                               aTGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGt                                                                                                               >  2:323104/1‑77 (MQ=255)

                                                               aTGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGt                                                                                                               <  1:323104/77‑1 (MQ=255)

                                                                tGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTg                                                >  1:329342/1‑139 (MQ=255)

                                                                 gcgcGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGc                                               <  2:844576/139‑1 (MQ=255)

                                                                      tAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCttt                                                             <  1:866163/120‑1 (MQ=255)

                                                                      tAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCttt                                                             >  2:866163/1‑120 (MQ=255)

                                                                        aTGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTtgatga                                        >  2:76604/1‑139 (MQ=255)

                                                                                 cccGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGa                               >  1:1042264/1‑139 (MQ=255)

                                                                                          aTGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCt                      <  1:76604/139‑1 (MQ=255)

                                                                                          aTGCG‑‑GCGCAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCCGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCt                      >  1:573075/1‑139 (MQ=255)

                                                                                                    cAAAGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCAg              >  1:641472/1‑139 (MQ=255)

                                                                                                     aaaGTTTGCTGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCAGa             <  2:467538/139‑1 (MQ=255)

                                                                                                              tGGCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCAGAAATCCTGAc    <  2:158080/139‑1 (MQ=255)

                                                                                                                gCAACCCACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCAGAAATCCTGACGc  <  2:1171501/139‑1 (MQ=255)

TCTCTGCGCCAGTGAAACTGGAATATAAGTGGAGCGATCAGCAACTGACCTTCCTGATGCGTCATGCGCGTAATGATTTCTCCCGCTGGGATGCG‑‑GCGCAAAGTTTGCTGGCAACCTACATCAAGCTGAACGTCGCGCGTCATCAGCAAGGTCAGCCGCTGTCTCTGCCGGTGCATGTGGCTGATGCTTTCCGCGCGGTACTGCTTGATGAGAAGATTGATCCAGCGCTGGCGGCAGAAATCCTGACGC  >  NC_000913/992228‑992476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`