Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,334,893 A→G intergenic (+64/‑231) cysB → / → ymiA N‑acetylserine‑responsive cysteine regulon transcriptional activator, autorepressor/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,334,8930AG100.0% 129.3 / NA 35intergenic (+64/‑231)cysB/ymiAN‑acetylserine‑responsive cysteine regulon transcriptional activator, autorepressor/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/18);  total (17/18)

TGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTACTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATT  >  NC_000913/1334757‑1335025
                                                                                                                                        |                                                                                                                                    
tGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                                    <  2:1170144/139‑1 (MQ=255)
tGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                                    >  2:251791/1‑139 (MQ=255)
tGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                                    <  1:684969/139‑1 (MQ=255)
    gCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTAtt                                                                                                                                >  2:509628/1‑139 (MQ=255)
       gTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCt                                                                                                                             <  2:402121/139‑1 (MQ=255)
                  ctctAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGcac                                                                                                                  <  1:509628/139‑1 (MQ=255)
                        tGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATAtt                                                                                                            >  1:269209/1‑139 (MQ=255)
                               aTTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                                    >  1:506873/1‑108 (MQ=255)
                               aTTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATcc                                                                                                     <  1:301591/139‑1 (MQ=255)
                               aTTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATcc                                                                                                     >  1:519397/1‑139 (MQ=255)
                                 tGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCt                                                                                                   <  2:857068/139‑1 (MQ=255)
                                    ggTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCat                                                                                                >  2:942530/1‑139 (MQ=255)
                                                    ataAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAAtt                                                                                <  1:251791/139‑1 (MQ=255)
                                                     taAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCat                                                                                                >  1:174041/1‑122 (MQ=255)
                                                     taAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCat                                                                                                <  2:174041/122‑1 (MQ=255)
                                                             ccGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                                    <  1:342988/78‑1 (MQ=255)
                                                             ccGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                                    >  1:522965/1‑78 (MQ=255)
                                                             ccGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                                    <  2:522965/78‑1 (MQ=255)
                                                             ccGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                                    >  2:342988/1‑78 (MQ=255)
                                                             ccGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTaaa                                                                       >  1:1154492/1‑139 (MQ=255)
                                                                               gCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCg                                                     <  2:519397/139‑1 (MQ=255)
                                                                                               cTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                                    >  2:202179/1‑44 (MQ=255)
                                                                                               cTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                                    >  1:632447/1‑44 (MQ=255)
                                                                                               cTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                                    <  2:632447/44‑1 (MQ=255)
                                                                                                 tttATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGttt                                   >  1:296607/1‑139 (MQ=255)
                                                                                                     tGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCag                               <  2:468390/139‑2 (MQ=255)
                                                                                                         gCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAag                                                                      >  2:480482/1‑96 (MQ=255)
                                                                                                         gCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAag                                                                      <  1:480482/96‑1 (MQ=255)
                                                                                                         gCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAatat                           >  1:1134240/1‑139 (MQ=255)
                                                                                                            aaGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAagtagt                                                                  <  2:208580/97‑1 (MQ=255)
                                                                                                            aaGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAagtagt                                                                  >  1:208580/1‑97 (MQ=255)
                                                                                                            aaGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAagta                                                                    <  1:805967/95‑1 (MQ=255)
                                                                                                            aaGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAagta                                                                    >  2:805967/1‑95 (MQ=255)
                                                                                                                                ctTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGaa    <  2:296607/139‑1 (MQ=255)
                                                                                                                                  tttGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAatt  <  2:1134240/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
TGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTACTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATT  >  NC_000913/1334757‑1335025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: