Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 105,107 T→C Y376H (TAT→CAT)  ftsA → ATP‑binding cell division FtsK recruitment protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913105,1070TC100.0% 221.1 / NA 57Y376H (TAT→CAT) ftsAATP‑binding cell division FtsK recruitment protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/32);  total (25/32)

ATTGTATTAACCGGTGGCGCAGCGCAGATCGAAGGTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATTATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTACAGCATCAGTTGGCTCGTGGATCAAGCGACTCAATAGTTGGCTGCGAAAAGAGTTT  >  NC_000913/104975‑105241
                                                                                                                                    |                                                                                                                                      
aTTGTATTAACCGGTGGCGCAGCGCAGATCGAAGGTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGa                                                                                                                                  <  1:1055042/139‑1 (MQ=255)
 ttGTATTAACCGGTGGCGCAGCGCAGATCGAAGGTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGAc                                                                                                                                 <  2:1038595/139‑1 (MQ=255)
     aTTAACCGGTGGCGCAGCGCAGATCGAAGGTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGAcggcg                                                                                                                             <  2:362658/139‑1 (MQ=255)
     aTTAACCGGTGGCGCAGCGCAGATCGAAGGTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGAcggcg                                                                                                                             <  2:743166/139‑1 (MQ=255)
         aCCGGTGGCGCAGCGCAGATCGAAGGTCTTGCAGCCGGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTgg                                                                                                                         <  2:167772/139‑1 (MQ=255)
              tGGCGCAGCGCAGATCGAAGGTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTg                                                                                                                    >  2:1158407/1‑139 (MQ=255)
              tGGCGCAGCGCAGATCGAAGGTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTg                                                                                                                    >  1:1171304/1‑139 (MQ=255)
              tGGCGCAGCGCAGATCGAAGGTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTg                                                                                                                    >  1:306335/1‑139 (MQ=255)
              tGGCGCAGCGCAGATCGAAGGTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCAGTTTAACGGATTATGCTCAGAAGCCGTATCATTCGACGGCGGTGGGATTg                                                                                                                    <  2:328269/139‑1 (MQ=255)
                                 ggTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGt                                                                                                 <  2:1171304/139‑1 (MQ=255)
                                 ggTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGt                                                                                                 <  1:429509/139‑1 (MQ=255)
                                  gTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGa                                                                                                                       >  2:784063/1‑116 (MQ=255)
                                  gTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGa                                                                                                                       <  1:784063/116‑1 (MQ=255)
                                     ttGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTcaca                                                                                             >  1:646885/1‑139 (MQ=255)
                                           ccTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTaa                                                                                       <  2:210869/139‑1 (MQ=255)
                                             tgtgCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGt                                                                                                 >  2:11810/1‑127 (MQ=255)
                                             tgtgCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGt                                                                                                 <  1:11810/127‑1 (MQ=255)
                                             tgtgCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACg                                                                                     >  1:129908/1‑139 (MQ=255)
                                                      cgcgTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTg                                                                            <  1:571877/139‑1 (MQ=255)
                                                      cgcgTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTg                                                                            <  1:1158407/139‑1 (MQ=255)
                                                         gtgtTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTca                                                                                               <  2:364808/117‑1 (MQ=255)
                                                         gtgtTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTca                                                                                               >  1:364808/1‑117 (MQ=255)
                                                         gtgtTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAg                                                                         >  1:504843/1‑139 (MQ=255)
                                                         gtgtTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAg                                                                         >  2:1157747/1‑139 (MQ=255)
                                                            tttCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAg                                                                      >  1:491737/1‑139 (MQ=255)
                                                                   cGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACg                                                               <  2:491737/139‑1 (MQ=255)
                                                                        gTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGa                                                                                                                       <  1:896163/78‑1 (MQ=255)
                                                                        gTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGa                                                                                                                       >  2:896163/1‑78 (MQ=255)
                                                                         tGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTAc                                                         <  2:217282/139‑1 (MQ=255)
                                                                           cGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCtt                                                                                                                 <  1:116760/81‑1 (MQ=255)
                                                                           cGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCtt                                                                                                                 >  2:116760/1‑81 (MQ=255)
                                                                              aTCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTACAGCAt                                                    <  1:1111277/139‑1 (MQ=255)
                                                                                      gccgcTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCtt                                                                                                                 <  2:289042/70‑1 (MQ=255)
                                                                                      gccgcTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCtt                                                                                                                 >  1:289042/1‑70 (MQ=255)
                                                                                                  tACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGAcggcg                                                                                                                             >  1:141579/1‑46 (MQ=255)
                                                                                                  tACCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGAcggcg                                                                                                                             <  2:141579/46‑1 (MQ=255)
                                                                                                   aCCGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTACAGCATCAGTTGGCTCGTGGATCAAGc                               <  2:646885/139‑1 (MQ=255)
                                                                                                    ccGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTACAGCATCAGTAGGCTCGTGGATCAAGCg                              >  2:637756/1‑139 (MQ=255)
                                                                                                     cGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCtt                                                                                                                 >  1:861901/1‑55 (MQ=255)
                                                                                                     cGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCtt                                                                                                                 <  2:861901/55‑1 (MQ=255)
                                                                                                     cGGTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTACAGCATCAGTTGGCTCGTGGATCAAGCGa                             <  2:306335/139‑1 (MQ=255)
                                                                                                       gTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTACAGCATCAGTTGGCTCGTGGATCAAGCGACt                           <  2:129908/139‑1 (MQ=255)
                                                                                                       gTTTAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTACAGCATCAGTTGGCTCGTGGATCAAGCGACt                           >  1:965408/1‑139 (MQ=255)
                                                                                                         ttAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGa                                                                                                       <  2:183394/61‑1 (MQ=255)
                                                                                                         ttAACGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGa                                                                                                       >  1:183394/1‑61 (MQ=255)
                                                                                                          tAACGGATTATGCTCAGGAGCCGTATCATTCGAcggcgg                                                                                                                            <  2:666113/39‑1 (MQ=255)
                                                                                                          tAACGGATTATGCTCAGGAGCCGTATCATTCGAcggcgg                                                                                                                            >  1:666113/1‑39 (MQ=255)
                                                                                                            aCGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGt                                                                                                 >  1:1085293/1‑64 (MQ=255)
                                                                                                            aCGGATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGt                                                                                                 <  2:1085293/64‑1 (MQ=255)
                                                                                                              ggATTATGCTCAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTACAGCATCAGTAGGCTCGTGGATCAAGCGACTCAATAGt                    <  1:637756/139‑1 (MQ=255)
                                                                                                                        cAGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTACAGCATCAGTTGGCTCGTGGATCAAGCGACTCAATAGTTGGCTGCGaa          <  1:1157747/139‑1 (MQ=255)
                                                                                                                         aGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTACAGCATCAGt                                                <  1:756088/100‑1 (MQ=255)
                                                                                                                         aGGAGCCGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTACAGCATCAGt                                                >  2:756088/1‑100 (MQ=255)
                                                                                                                              ccGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAg                                                                               <  2:400601/64‑1 (MQ=255)
                                                                                                                              ccGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAg                                                                               >  1:400601/1‑64 (MQ=255)
                                                                                                                               cGTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTACAGCATCAGTTGGCTCGTGGATCAAGCGACTCAATAGTTGGCTGCGAAAAGAGtt   >  2:542360/1‑139 (MQ=255)
                                                                                                                                gTATCATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTACAGCATCAGTTGGCTCGTGGATCAAGCGACTCAATAGTTGGCTGCGAAAAGAGttt  >  2:903509/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                      
ATTGTATTAACCGGTGGCGCAGCGCAGATCGAAGGTCTTGCAGCCTGTGCTCAGCGCGTGTTTCATACGCAAGTGCGTATCGGCGCGCCGCTGAACATTACCGGTTTAACGGATTATGCTCAGGAGCCGTATTATTCGACGGCGGTGGGATTGCTTCACTATGGGAAAGAGTCACATCTTAACGGTGAAGCTGAAGTAGAAAAACGTGTTACAGCATCAGTTGGCTCGTGGATCAAGCGACTCAATAGTTGGCTGCGAAAAGAGTTT  >  NC_000913/104975‑105241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑