Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,693	A→G	L23P (CTC→CCC)	ycjD ←	DUF559 family endonuclease‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,693	0	A	G	100.0%	131.2 / NA	36	L23P (CTC→CCC)	ycjD	DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/20);  total (16/20)

TGCTGCCCACCATCCAGCTCAACGACTACACGCGCCGAGCAGCAAGCAAAATCGAGAATGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGG  >  NC_000913/1351564‑1351814                                                                                                                                  |                                                                                                                          tgctgcCCACCATCCAGCTCAACGACTACACGCGCCGAGCAGCAAGCAAAATCGAGAATGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGtt                                                                                                                  <  1:1119334/139‑1 (MQ=255)   ctgcCCACCATCCAGCTCAACGACTACACGCGCCGAGCAGCAAGCAAAATCGAGAATGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTcc                                                                                                                <  1:289151/139‑1 (MQ=255)          ccaTCCAGCTCAACGACTACACGCGCCGAGCAGCAAGCAAAATCGAGAATGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAg                                                                                                         <  1:116697/139‑1 (MQ=255)                                 cgcCGAGCAGCAAGCAAAATCGAGAATGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCAcgc                                                                                  >  1:1047344/1‑139 (MQ=255)                                    cGAGCAGCAAGCAAAATCGAGAATGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCa                                                                               >  2:626679/1‑139 (MQ=255)                                     gAGCAGCAAGCAAAATCGAGAATGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCAt                                                                              <  2:108602/139‑1 (MQ=255)                                     gAGCAGCAAGCAAAATCGAGAATGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCAt                                                                              >  1:507637/1‑139 (MQ=255)                                                aaaaTCGAGAATGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAAtt                                                                   >  2:171155/1‑139 (MQ=255)                                                 aaaTCGAGAATGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAAttt                                                                  <  1:171155/139‑1 (MQ=255)                                                   aTCGAGAATGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTta                                                                <  2:319320/139‑1 (MQ=255)                                                       agaATGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTtatcta                                                            <  1:626679/139‑1 (MQ=255)                                                         aaTGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTtatctatc                                                          >  1:721495/1‑139 (MQ=255)                                                           tGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCAc                                                        <  2:399743/139‑1 (MQ=255)                                                                 tCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCAtt                                                  <  1:960098/139‑1 (MQ=255)                                                                  ccccACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTc                                                 >  1:478102/1‑139 (MQ=255)                                                                  ccccACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCCTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTc                                                 >  2:785028/1‑139 (MQ=255)                                                                      aCTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGAc                                             <  2:721495/139‑1 (MQ=255)                                                                       cTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTg                                                                                                              >  2:647940/1‑73 (MQ=255)                                                                       cTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTg                                                                                                              <  1:647940/73‑1 (MQ=255)                                                                                   ggCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCtt                                <  1:407235/139‑1 (MQ=255)                                                                                    gCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCAtt                                                  <  1:362431/120‑1 (MQ=255)                                                                                    gCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCAtt                                                  >  2:362431/1‑120 (MQ=255)                                                                                    gCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCttt                               >  1:506978/1‑139 (MQ=255)                                                                                           ttGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGa                        <  2:643715/139‑1 (MQ=255)                                                                                             gAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCCTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCGGAAt                      <  1:785028/139‑1 (MQ=255)                                                                                                             cGGCTGCGAAGGTATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTgg      >  2:637303/1‑139 (MQ=255)                                                                                                             cGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGAttt                                      <  2:195296/107‑1 (MQ=255)                                                                                                             cGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGAttt                                      >  1:195296/1‑107 (MQ=255)                                                                                                             cGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTgg      >  1:69493/1‑139 (MQ=255)                                                                                                             cGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTgg      <  2:507637/139‑1 (MQ=255)                                                                                                             cGGCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTgg      >  1:637858/1‑139 (MQ=255)                                                                                                              ggCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGa               <  2:86311/129‑1 (MQ=255)                                                                                                              ggCTGCGAAGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGa               >  1:86311/1‑129 (MQ=255)                                                                                                                 tGCGAAGATATCGCCAGGGCTATCGTTCCTGCAAAGTGAGAATGCATATTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTAACAAGATTCAATCTTTTGTCAGAATGAGTTGAATAATCTGGGagg  <  1:126817/139‑1 (MQ=255)                                                                                                                     aaGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCAtt                                                  <  1:80249/87‑1 (MQ=255)                                                                                                                     aaGATATCGCCAGGGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCAtt                                                  >  2:80249/1‑87 (MQ=255)                                                                                                                                  |                                                                                                                          TGCTGCCCACCATCCAGCTCAACGACTACACGCGCCGAGCAGCAAGCAAAATCGAGAATGTAGCTCCCCACTGGATGTTGACGGCGAAATTTGAAATCACTAAAACGTCGGCTGCGAAGATATCGCCAGAGCTTTCGTTCCTGCAAAGTGAGATTGCGTCTTAAATCACGCGCATTTGATTTAATTTTATCTATCACCTCATTCTGACAAGATTTAATCTTTTGTCAGAATGAGGTGAATAATCTGGAAGG  >  NC_000913/1351564‑1351814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑