Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,585,424 A→G Y355H (TAT→CAT)  ydeP ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,585,4240AG100.0% 110.8 / NA 30Y355H (TAT→CAT) ydePputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/13);  total (17/13)

GATACCCGCACCAGGCTTGCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATC  >  NC_000913/1585290‑1585556
                                                                                                                                      |                                                                                                                                    
gATACCCGCACCAGGCTTGCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCg                                                                                                                                  <  1:904040/139‑1 (MQ=255)
    cccGCACCAGGCTTGCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAg                                                                                                                              >  2:708552/1‑139 (MQ=255)
     ccGCACCAGGCTTGCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGc                                                                                                                             <  1:636350/139‑1 (MQ=255)
           cAGGCTTGCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTc                                                                                                                       >  2:590157/1‑139 (MQ=255)
             ggCTTGCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTAAGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGACATGTGCGTCAGCCAGTTCgg                                                                                                                     <  1:708552/139‑1 (MQ=255)
                  gCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGAt                                                                                                                 <  1:203846/138‑1 (MQ=255)
                  gCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGAt                                                                                                                 >  2:203846/1‑138 (MQ=255)
                             cTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACt                                                                                                     <  1:590157/139‑1 (MQ=255)
                                              ttGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGAGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGtt                                                                                    <  2:127082/139‑1 (MQ=255)
                                               tGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACt                                                                                                     <  1:1091462/121‑1 (MQ=255)
                                               tGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACt                                                                                                     >  2:1091462/1‑121 (MQ=255)
                                                                          cATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGtt                                                                                    >  2:765285/1‑111 (MQ=255)
                                                                          cATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGtt                                                                                    <  1:765285/111‑1 (MQ=255)
                                                                          cATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAAc                                                        >  2:801779/1‑139 (MQ=255)
                                                                                 gTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACgg                                                 >  1:673566/1‑139 (MQ=255)
                                                                                       aGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGAt                                                                                                                 >  2:1179308/1‑69 (MQ=255)
                                                                                       aGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGAt                                                                                                                 <  1:1179308/69‑1 (MQ=255)
                                                                                       aGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACt                                                                                                     <  1:494167/81‑1 (MQ=255)
                                                                                       aGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACt                                                                                                     >  2:494167/1‑81 (MQ=255)
                                                                                                       tAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGAttt                                                                                                               >  2:469614/1‑55 (MQ=255)
                                                                                                       tAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGAttt                                                                                                               <  1:469614/55‑1 (MQ=255)
                                                                                                       tAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGa                                                                                 <  1:596032/85‑1 (MQ=255)
                                                                                                       tAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGa                                                                                 >  2:596032/1‑85 (MQ=255)
                                                                                                       tAACAGATAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACgg                           >  1:548255/1‑139 (MQ=255)
                                                                                                             aTAATGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGGGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCg                     >  2:312867/1‑139 (MQ=255)
                                                                                                                 tGGTGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTg                 >  1:1143681/1‑139 (MQ=255)
                                                                                                                    tGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTgtgt                                                                                                           <  2:1029510/46‑1 (MQ=255)
                                                                                                                    tGCGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTgtgt                                                                                                           >  1:1029510/1‑46 (MQ=255)
                                                                                                                      cGTTCGGCAGCGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATaa            >  2:297794/1‑139 (MQ=255)
                                                                                                                                cGGCATGTGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGtcatc  >  2:703837/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                    
GATACCCGCACCAGGCTTGCCAATGTTACCTTTCATCAACAGCAGATTGACCAGTTGCTGTACGTTCTGGGTACCATGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATC  >  NC_000913/1585290‑1585556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: