Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,719,281	C→T	V108M (GTG→ATG)	anmK ←	anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,719,281	0	C	T	100.0%	175.7 / NA	51	V108M (GTG→ATG)	anmK	anhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/25);  total (26/25)

GGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGC  >  NC_000913/1719149‑1719411                                                                                                                                     |                                                                                                                                   ggAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGt                                                                                                                              <  2:199305/139‑1 (MQ=255)     cGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGt                                                                                                                          <  1:463204/139‑1 (MQ=255)        aGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCa                                                                                                                       >  2:631939/1‑139 (MQ=255)             ccAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCa                                                                                                                  <  1:631939/139‑1 (MQ=255)                    tGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGtt                                                                                                           >  2:297984/1‑139 (MQ=255)                    tGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCAACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGtt                                                                                                           >  1:547632/1‑139 (MQ=255)                      cgcCTTGTCCTCCCAAGGCGATATCGCGCCGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCCCAATTTGATTGTTATCGCAAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTg                                                                                                         >  1:715554/1‑139 (MQ=255)                      cgcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTg                                                                                                         <  2:561041/139‑1 (MQ=255)                      cgcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTg                                                                                                         <  2:270695/139‑1 (MQ=255)                        ccTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGAc                                                                                                       <  2:539387/139‑1 (MQ=255)                              cctccCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGAc                                                                                                 >  2:910375/1‑139 (MQ=255)                                tccCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACaa                                                                                               >  2:240325/1‑139 (MQ=255)                                     aGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGAt                                                                                          <  2:556943/139‑1 (MQ=255)                                        cAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGc                                                                                       <  1:297984/139‑1 (MQ=255)                                           tatCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAAc                                                                                    <  2:639312/139‑1 (MQ=255)                                           tatCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAAc                                                                                    >  2:965855/1‑139 (MQ=255)                                                cgACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGa                                                                                                        >  2:712921/1‑114 (MQ=255)                                                cgACGGCGAAAATCACCGACAACCGTAATTCCGGAGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGa                                                                                                        <  1:712921/114‑1 (MQ=255)                                                  accgCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCATGACAACCGATCGCAACTATAtcc                                                                             >  1:864450/4‑138 (MQ=255)                                                                  aCAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGAtttt                                                             <  2:53783/139‑1 (MQ=255)                                                                       cGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTcc                                                        <  2:780764/139‑1 (MQ=255)                                                                             tCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGc                                                  <  1:965855/139‑1 (MQ=255)                                                                                ggTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAg                                               >  1:1042258/1‑139 (MQ=255)                                                                                      tgcCACATTTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGACGGTTCATGCCAGACGGTTTGACCGTGACAACCGATc                                                                                         <  2:1147939/91‑1 (MQ=255)                                                                                      tgcCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATc                                                                                         >  1:1147939/1‑91 (MQ=255)                                                                                      tgcCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCt                                                       >  2:574358/1‑125 (MQ=255)                                                                                      tgcCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCt                                                       <  1:574358/125‑1 (MQ=255)                                                                                                     gTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCaaaa                          >  2:66349/1‑139 (MQ=255)                                                                                                      ttATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCt                                                       <  2:629957/109‑1 (MQ=255)                                                                                                      ttATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCt                                                       >  1:629957/1‑109 (MQ=255)                                                                                                                         tgtgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTAGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATc      >  2:625529/1‑139 (MQ=255)                                                                                                                          gtgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAAcc                                                                                             >  2:289268/1‑51 (MQ=255)                                                                                                                          gtgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAAcc                                                                                             <  1:289268/51‑1 (MQ=255)                                                                                                                          gtgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGAtttt                                                             >  2:486378/1‑83 (MQ=255)                                                                                                                          gtgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGAtttt                                                             <  1:486378/83‑1 (MQ=255)                                                                                                                           tgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGc                                                                                       >  2:872945/1‑56 (MQ=255)                                                                                                                           tgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGc                                                                                       <  1:872945/56‑1 (MQ=255)                                                                                                                           tgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACta                                                                                  <  1:851095/61‑1 (MQ=255)                                                                                                                           tgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACta                                                                                  >  2:851095/1‑61 (MQ=255)                                                                                                                           tgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGc                    <  2:406202/123‑1 (MQ=255)                                                                                                                           tgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGc                    >  1:406202/1‑123 (MQ=255)                                                                                                                           tgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATc      <  1:372045/137‑1 (MQ=255)                                                                                                                           tgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATc      >  2:372045/1‑137 (MQ=255)                                                                                                                           tgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCaa    >  2:885214/1‑139 (MQ=255)                                                                                                                           tgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCaa    >  2:227319/1‑139 (MQ=255)                                                                                                                            gtggtgCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAg   <  1:421018/139‑1 (MQ=255)                                                                                                                            gtggtgCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAg   >  1:394007/1‑139 (MQ=255)                                                                                                                            gtggtgCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAg   >  1:112403/1‑139 (MQ=255)                                                                                                                             tggtgCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGc  <  1:227319/139‑1 (MQ=255)                                                                                                                              ggtgCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTc                                                         <  2:640826/83‑1 (MQ=255)                                                                                                                              ggtgCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTc                                                         >  1:640826/1‑83 (MQ=255)                                                                                                                                     |                                                                                                                                   GGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGC  >  NC_000913/1719149‑1719411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑