Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,825,480	T→C	D49G (GAC→GGC)	spy ←	periplasmic ATP‑independent protein refolding chaperone, stress‑induced

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,825,480	0	T	C	100.0%	171.6 / NA	45	D49G (GAC→GGC)	spy	periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/22);  total (23/22)

ATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTT  >  NC_000913/1825344‑1825614                                                                                                                                         |                                                                                                                                       aTCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGccc                                                                                                                                      >  2:1036423/1‑139 (MQ=255)          aaTGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGa                                                                                                                             <  2:270035/139‑1 (MQ=255)              atgTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGa                                                                                                                         >  1:1076046/1‑139 (MQ=255)               tgTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGaa                                                                                                                        <  2:1117146/139‑1 (MQ=255)                  cATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGACTTTGAACATCATGCCCTGATGCGTACCGAACtt                                                                                                                     >  2:755152/1‑139 (MQ=255)                   aTGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTg                                                                                                                    >  1:801472/1‑139 (MQ=255)                   aTGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTg                                                                                                                    >  1:1048365/1‑139 (MQ=255)                        ttGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCttt                                                                                                               <  2:1076046/139‑1 (MQ=255)                           cgcgGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTtgtg                                                                                                            >  1:681823/1‑139 (MQ=255)                               gcgTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGc                                                                                                        <  1:951144/139‑1 (MQ=255)                                    cttcCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGcatcat                                                                                                   <  1:755152/139‑1 (MQ=255)                                     ttcCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCatcatc                                                                                                  >  2:1005188/1‑139 (MQ=255)                                         aGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCt                                                                                              <  1:3246/139‑1 (MQ=255)                                             gcggACGTTTCATCTGGTCACGCTGGCCTTTCATGCTTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTcgc                                                                                          >  2:922269/1‑139 (MQ=255)                                              cggACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTcgcg                                                                                         >  2:614845/1‑139 (MQ=255)                                                   gTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGc                                                                                    <  1:922269/139‑1 (MQ=255)                                                      tCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCgg                                                                                 >  1:1028132/1‑139 (MQ=255)                                                              tCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCgg                                                                         <  1:1036423/139‑1 (MQ=255)                                                              tCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCgg                                                                         <  2:1028132/139‑1 (MQ=255)                                                                     ggCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAgtggt                                                                  >  1:491781/1‑139 (MQ=255)                                                                       ccTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGgtgt                                                                >  2:395926/1‑139 (MQ=255)                                                                          ttCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTg                                                             <  2:801472/139‑1 (MQ=255)                                                                                          tCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGtt                                             >  2:27453/1‑139 (MQ=255)                                                                                           ctgctgTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGtgc                                                                              <  2:980357/105‑1 (MQ=255)                                                                                           ctgctgTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGtgc                                                                              >  1:980357/1‑105 (MQ=255)                                                                                             gctgTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGc                                          <  1:824279/139‑1 (MQ=255)                                                                                               tgTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCcg                                        <  1:83481/139‑1 (MQ=255)                                                                                                   tCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGcatca                                                                                                    <  2:882045/75‑1 (MQ=255)                                                                                                   tCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGcatca                                                                                                    >  1:882045/1‑75 (MQ=255)                                                                                                      gcgcGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAag                                 >  2:373468/1‑139 (MQ=255)                                                                                                         cgTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGc                              <  2:681823/139‑1 (MQ=255)                                                                                                           tCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCa                                                        <  2:552325/111‑1 (MQ=255)                                                                                                           tCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCa                                                        >  1:552325/1‑111 (MQ=255)                                                                                                           tCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCa                            >  1:358171/1‑139 (MQ=255)                                                                                                                      caggtCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGc                              <  1:191801/126‑1 (MQ=255)                                                                                                                      caggtCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGc                              >  2:191801/1‑126 (MQ=255)                                                                                                                         gtCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGc                                                                                    >  1:192957/1‑69 (MQ=255)                                                                                                                         gtCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGc                                                                                    <  2:192957/69‑1 (MQ=255)                                                                                                                         gtCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCa                                                        <  1:819379/97‑1 (MQ=255)                                                                                                                         gtCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCa                                                        >  2:819379/1‑97 (MQ=255)                                                                                                                           cTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCaacaaa            <  1:1005188/139‑1 (MQ=255)                                                                                                                             ttGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAATGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCaacaaaca          >  1:721806/1‑139 (MQ=255)                                                                                                                              tGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAg         <  2:358171/139‑1 (MQ=255)                                                                                                                                   atcatGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTag                      >  2:316908/1‑121 (MQ=255)                                                                                                                                   atcatGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTag                      <  1:316908/121‑1 (MQ=255)                                                                                                                                     catGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGtt  >  2:936637/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                       ATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAACAGTGCAGTT  >  NC_000913/1825344‑1825614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑