Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,787	T→C	S34P (TCT→CCT)	mntP →	putative Mn(2+) efflux pump, mntR‑regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,787	0	T	C	100.0%	241.8 / NA	64	S34P (TCT→CCT)	mntP	putative Mn(2+) efflux pump, mntR‑regulated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/31);  total (33/31)

TATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGAT  >  NC_000913/1905661‑1905920                                                                                                                               |                                                                                                                                      tATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGc                                                                                                                           >  1:899865/1‑139 (MQ=255)        aCCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCgg                                                                                                                    >  1:981136/1‑139 (MQ=255)          cccGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGcc                                                                                                                  >  2:1180569/1‑139 (MQ=255)           ccGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCt                                                                                                                 <  1:993502/139‑1 (MQ=255)            cGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCtt                                                                                                                <  1:567477/139‑1 (MQ=255)              gTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTg                                                                                                                                    <  1:1105579/117‑1 (MQ=255)              gTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTg                                                                                                                                    >  2:1105579/1‑117 (MQ=255)               tCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCt                                                                                                                 <  1:1181090/135‑1 (MQ=255)               tCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCt                                                                                                                 >  2:1181090/1‑135 (MQ=255)                   gACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTg                                                                                                         >  1:943525/1‑139 (MQ=255)                    aCATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCACCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTgg                                                                                                        >  1:144248/1‑139 (MQ=255)                             tGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGa                                                                                               >  1:1104419/1‑139 (MQ=255)                             tGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGa                                                                                               <  2:723836/139‑1 (MQ=255)                               aaTATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGaaa                                                                                             >  2:276597/1‑139 (MQ=255)                                    cACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTg                                                                                                                                    >  1:212230/1‑95 (MQ=255)                                    cACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTg                                                                                                                                    <  2:212230/95‑1 (MQ=255)                                        gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgc                                                                                    <  1:1180569/139‑1 (MQ=255)                                        gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgc                                                                                    <  2:981136/139‑1 (MQ=255)                                          tACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgccg                                                                                  >  1:343844/1‑139 (MQ=255)                                            cTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCt                                                                                >  2:758237/1‑139 (MQ=255)                                               ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                             >  1:1018390/1‑139 (MQ=255)                                               ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                             <  1:276597/139‑1 (MQ=255)                                               ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                             >  1:1016879/1‑139 (MQ=255)                                                    cttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCt                                                                        <  1:1134570/139‑1 (MQ=255)                                                     ttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTg                                                                       <  2:1104419/139‑1 (MQ=255)                                                      tGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAAc                                                                                                                       <  2:355271/90‑1 (MQ=255)                                                      tGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAAc                                                                                                                       >  1:355271/1‑90 (MQ=255)                                                         gTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGa                                                                   <  2:899865/139‑1 (MQ=255)                                                            tGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATg                                                                >  2:1047876/1‑139 (MQ=255)                                                              gTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATggg                                                              <  2:1016879/139‑1 (MQ=255)                                                                       tggatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGaa                                                                  <  2:840831/126‑1 (MQ=255)                                                                       tggatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGaa                                                                  >  1:840831/1‑126 (MQ=255)                                                                         gatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagcca                                                   >  1:1051923/1‑139 (MQ=255)                                                                           tgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCAAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagccagc                                                 >  2:702553/1‑139 (MQ=255)                                                                             cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCg                                               >  1:318270/1‑139 (MQ=255)                                                                                  gctgcATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTg                                          <  1:500824/139‑1 (MQ=255)                                                                                      cATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCGTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGGCCt                                      <  1:758237/139‑1 (MQ=255)                                                                                            tCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTA‑TTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATgg                               <  2:271168/139‑1 (MQ=255)                                                                                             cGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATgg                               <  2:1051923/139‑1 (MQ=255)                                                                                                tAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGtt                                            <  2:410543/123‑1 (MQ=255)                                                                                                tAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGtt                                            >  1:410543/1‑123 (MQ=255)                                                                                                  aaGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCa                          <  2:109495/139‑1 (MQ=255)                                                                                                      tGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGt                                             <  1:326097/116‑1 (MQ=255)                                                                                                      tGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGt                                             >  2:326097/1‑116 (MQ=255)                                                                                                        ccACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTg                                                                                                      <  1:52437/57‑1 (MQ=255)                                                                                                        ccACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTg                                                                                                      >  2:52437/1‑57 (MQ=255)                                                                                                        ccACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGAt                    >  2:552726/1‑139 (MQ=255)                                                                                                        ccACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGAt                    <  2:1018390/139‑1 (MQ=255)                                                                                                         cACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTcc                                       >  2:967441/1‑119 (MQ=255)                                                                                                         cACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTcc                                       <  1:967441/119‑1 (MQ=255)                                                                                                         cACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGAtt                   >  1:902422/1‑139 (MQ=255)                                                                                                             cTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGt               <  2:343844/139‑1 (MQ=255)                                                                                                                cATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGt                                                                                                       >  2:938928/1‑48 (MQ=255)                                                                                                                cATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGt                                                                                                       <  1:938928/48‑1 (MQ=255)                                                                                                                cATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGa                                                                              >  2:911944/1‑73 (MQ=255)                                                                                                                cATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGa                                                                              <  1:911944/73‑1 (MQ=255)                                                                                                                  tAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtgtg          >  2:580950/1‑139 (MQ=255)                                                                                                                     aCCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagc                                                     <  2:494662/93‑1 (MQ=255)                                                                                                                     aCCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagc                                                     >  1:494662/1‑93 (MQ=255)                                                                                                                          aaTTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGa                                                                                               <  2:1164842/46‑1 (MQ=255)                                                                                                                          aaTTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGa                                                                                               >  1:1164842/1‑46 (MQ=255)                                                                                                                          aaTTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATgg                               <  2:668730/110‑1 (MQ=255)                                                                                                                          aaTTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATgg                               >  1:668730/1‑110 (MQ=255)                                                                                                                          aaTTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat  >  2:630293/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                      TATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGAT  >  NC_000913/1905661‑1905920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑