Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,995,769 T→C intergenic (‑410/‑49) uvrY ← / → yecF response regulator in two‑component regulatory system with BarA/DUF2594 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,995,7690TC100.0% 323.1 / NA 85intergenic (‑410/‑49)uvrY/yecFresponse regulator in two‑component regulatory system with BarA/DUF2594 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (49/36);  total (49/36)

ACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGAT  >  NC_000913/1995633‑1995903
                                                                                                                                        |                                                                                                                                      
aCAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGTTATCACGCTTAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTa                                                                                                                                      >  1:23018/1‑139 (MQ=255)
         gCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTc                                                                                                                             >  2:1128943/1‑139 (MQ=255)
         gCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTc                                                                                                                             >  1:166745/1‑139 (MQ=255)
         gCAAAAAACATACATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTc                                                                                                                             >  2:862417/1‑139 (MQ=255)
              aaaCATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGc                                                                                                                        >  1:1144361/1‑139 (MQ=255)
                          tAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTg                                                                                                            <  2:166745/139‑1 (MQ=255)
                                        ggTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGa                                                                                              <  2:23018/139‑1 (MQ=255)
                                                      cGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCg                                                                                                                         >  2:385988/1‑98 (MQ=255)
                                                      cGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCg                                                                                                                         <  1:385988/98‑1 (MQ=255)
                                                      cGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCAt                                                                                                              >  1:585512/1‑109 (MQ=255)
                                                      cGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCAt                                                                                                              <  2:585512/109‑1 (MQ=255)
                                                          ttttATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGTCGAGGaaa                                                                                            <  2:958247/123‑1 (MQ=255)
                                                          ttttATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACg                                                                                                  >  1:897282/1‑117 (MQ=255)
                                                          ttttATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACg                                                                                                  <  2:897282/117‑1 (MQ=255)
                                                          ttttATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGaaa                                                                                            >  1:958247/1‑123 (MQ=255)
                                                          ttttATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGa                                                                                              >  1:347535/1‑121 (MQ=255)
                                                          ttttATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGa                                                                                              <  2:347535/121‑1 (MQ=255)
                                                          ttttATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTAc                                                                                >  2:428034/1‑135 (MQ=255)
                                                          ttttATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTAc                                                                                <  1:428034/135‑1 (MQ=255)
                                                          ttttATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCt                                                                            >  2:446500/1‑139 (MQ=255)
                                                          ttttATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCt                                                                            >  1:487106/1‑139 (MQ=255)
                                                               taGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGAttt                                                                       >  2:285023/1‑139 (MQ=255)
                                                                    cTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAg                                                                                                >  1:237408/1‑109 (MQ=255)
                                                                    cTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAg                                                                                                <  2:237408/109‑1 (MQ=255)
                                                                    cTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa                                                                  <  1:1128943/139‑1 (MQ=255)
                                                                     tGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACAttttt                                                                                                                              >  2:925087/1‑78 (MQ=255)
                                                                     tGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACAttttt                                                                                                                              <  1:925087/78‑1 (MQ=255)
                                                                     tGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCAt                                                                                                              <  2:367100/94‑1 (MQ=255)
                                                                     tGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCAt                                                                                                              >  1:367100/1‑94 (MQ=255)
                                                                     tGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa                                                                  <  2:813678/138‑1 (MQ=255)
                                                                     tGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa                                                                  >  1:813678/1‑138 (MQ=255)
                                                                       ccGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAg                                                                                                >  2:299151/1‑106 (MQ=255)
                                                                       ccGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAg                                                                                                <  1:299151/106‑1 (MQ=255)
                                                                       ccGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAg                                                                                                <  2:903207/106‑1 (MQ=255)
                                                                       ccGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAg                                                                                                >  1:903207/1‑106 (MQ=255)
                                                                       ccGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTg                                                               >  2:821462/1‑139 (MQ=255)
                                                                       ccGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTg                                                               >  1:1143508/1‑139 (MQ=255)
                                                                       ccGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCACAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTg                                                               >  2:528322/1‑139 (MQ=255)
                                                                            atCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACAttttt                                                                                                                              >  2:301440/1‑71 (MQ=255)
                                                                            atCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACAttttt                                                                                                                              <  1:301440/71‑1 (MQ=255)
                                                                            atCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCgag                                                          >  1:160995/1‑139 (MQ=255)
                                                                                cGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACg                                                                                                  >  1:212885/1‑95 (MQ=255)
                                                                                cGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACg                                                                                                  <  2:212885/95‑1 (MQ=255)
                                                                                 gCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa                                                                  >  2:469372/1‑126 (MQ=255)
                                                                                 gCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa                                                                  <  1:469372/126‑1 (MQ=255)
                                                                                 gCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGaataa                                                     >  1:856761/1‑139 (MQ=255)
                                                                                 gCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGaa                                                        >  1:1079360/1‑136 (MQ=255)
                                                                                 gCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGaa                                                        <  2:1079360/136‑1 (MQ=255)
                                                                                   tCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAg                                                                                                >  1:840265/1‑94 (MQ=255)
                                                                                   tCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAg                                                                                                <  2:840265/94‑1 (MQ=255)
                                                                                     aaaaaaCAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCaa                                                 >  1:703544/1‑139 (MQ=255)
                                                                                               cACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCgaga                                                         <  2:254540/121‑1 (MQ=255)
                                                                                               cACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCgaga                                                         >  1:254540/1‑121 (MQ=255)
                                                                                                            aGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAaaataa                                                                                        >  2:883168/1‑77 (MQ=255)
                                                                                                            aGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAaaataa                                                                                        <  1:883168/77‑1 (MQ=255)
                                                                                                               aaaGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTcctgcct                       <  2:1144361/139‑1 (MQ=255)
                                                                                                                   cTTATGGTATACTCCGCCGCCCTAACATTTTTCAc                                                                                                                           <  1:55032/35‑1 (MQ=255)
                                                                                                                   cTTATGGTATACTCCGCCGCCCTAACATTTTTCAc                                                                                                                           >  2:55032/1‑35 (MQ=255)
                                                                                                                   cTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGaaa                   >  1:54852/1‑139 (MQ=255)
                                                                                                                    ttATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAg                  <  2:1143508/139‑1 (MQ=255)
                                                                                                                            taCTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGa                                                                                              <  2:1154897/55‑1 (MQ=255)
                                                                                                                            taCTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGa                                                                                              >  1:1154897/1‑55 (MQ=255)
                                                                                                                             aCTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGa                                                                                                    <  2:782776/48‑1 (MQ=255)
                                                                                                                             aCTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGa                                                                                                    >  1:782776/1‑48 (MQ=255)
                                                                                                                             aCTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCgaga                                                         >  1:339603/1‑91 (MQ=255)
                                                                                                                             aCTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCgaga                                                         <  2:339603/91‑1 (MQ=255)
                                                                                                                             aCTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGa         >  2:799528/1‑139 (MQ=255)
                                                                                                                              cTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCt                                                                            >  1:1052863/1‑71 (MQ=255)
                                                                                                                              cTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCt                                                                            <  2:1052863/71‑1 (MQ=255)
                                                                                                                               tCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCt                                                                            <  2:549423/70‑1 (MQ=255)
                                                                                                                               tCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCt                                                                            >  1:549423/1‑70 (MQ=255)
                                                                                                                                 cgccgcCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAg                                                                                   <  2:463974/61‑1 (MQ=255)
                                                                                                                                 cgccgcCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAg                                                                                   >  1:463974/1‑61 (MQ=255)
                                                                                                                                 cgccgcCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa                                                                  <  2:542018/78‑1 (MQ=255)
                                                                                                                                 cgccgcCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa                                                                  >  1:542018/1‑78 (MQ=255)
                                                                                                                                 cgccgcCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGcc                                                             <  2:483409/83‑1 (MQ=255)
                                                                                                                                 cgccgcCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGcc                                                             >  1:483409/1‑83 (MQ=255)
                                                                                                                                 cgccgcCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCt     >  2:415382/1‑139 (MQ=255)
                                                                                                                                 cgccgcCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCt     <  2:856761/139‑1 (MQ=255)
                                                                                                                                  gccgccCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTg    >  1:635777/1‑139 (MQ=255)
                                                                                                                                   ccgccCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGaaa                                                                                            >  1:1004527/1‑50 (MQ=255)
                                                                                                                                   ccgccCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGaaa                                                                                            <  2:1004527/50‑1 (MQ=255)
                                                                                                                                    cgccCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCt                                                                            >  1:693589/1‑65 (MQ=255)
                                                                                                                                    cgccCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCt                                                                            <  2:693589/65‑1 (MQ=255)
                                                                                                                                    cgccCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGAt  >  1:435307/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGAT  >  NC_000913/1995633‑1995903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑