Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,072,728 G→A G397D (GGT→GAT)  flu → CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,072,7280GA99.7% 1081.0 / NA 300G397D (GGT→GAT) fluCP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (143/156);  minor base T (0/1);  total (143/157)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 300 total aligned reads displayed.

CTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACGGTAAATGGCGGACTGTT  >  NC_000913/2072592‑2072860
                                                                                                                                        |                                                                                                                                    
cTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATa                                                                                                                                    >  2:754693/1‑139 (MQ=255)
cTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATa                                                                                                                                    >  2:384628/1‑139 (MQ=255)
cTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATa                                                                                                                                    >  2:20494/1‑139 (MQ=255)
  gACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATAcc                                                                                                                                  <  2:513754/139‑1 (MQ=255)
  gACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATAcc                                                                                                                                  <  2:310179/139‑1 (MQ=255)
      ggACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGa                                                                                                                              >  2:782816/1‑139 (MQ=255)
         cacacaGCCACTAATACCTGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCg                                                                                                                           <  2:793125/139‑1 (MQ=255)
              tgCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGAGAAATGGCGGTGTACTGCTGGCCGATTCCGGGGCCGCTGTCAGTGATACCCGGAGCGACGGa                                                                                                                      <  1:1134496/138‑1 (MQ=255)
              aGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGa                                                                                                                      <  1:836735/139‑1 (MQ=255)
               gCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGaa                                                                                                                     <  1:1017280/139‑1 (MQ=255)
               gCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGaa                                                                                                                     <  2:1047586/139‑1 (MQ=255)
               gCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGaa                                                                                                                     <  1:782816/139‑1 (MQ=255)
                 cACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCAACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAg                                                                                                                   <  1:696641/139‑1 (MQ=255)
                    tAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCa                                                                                                                <  2:349678/139‑1 (MQ=255)
                    tAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCa                                                                                                                <  1:1151780/139‑1 (MQ=255)
                      aTACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCAtt                                                                                                              >  2:665028/1‑139 (MQ=255)
                       tACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTc                                                                                                             <  1:384470/139‑1 (MQ=255)
                            gcgTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTc                                                                                                             <  2:917361/134‑1 (MQ=255)
                            gcgTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTAt                                                                                                        >  1:159022/1‑139 (MQ=255)
                            gcgTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTAt                                                                                                        >  2:149461/1‑139 (MQ=255)
                              gTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATAccc                                                                                                                                 >  1:985567/1‑112 (MQ=255)
                              gTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCg                                                                                                      >  1:21146/1‑139 (MQ=255)
                              gTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCg                                                                                                      >  1:301873/1‑139 (MQ=255)
                              gTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCg                                                                                                      >  1:587342/1‑139 (MQ=255)
                              gTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCg                                                                                                      >  1:795465/1‑139 (MQ=255)
                                 gatgatGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAg                                                                                                   >  1:617867/1‑139 (MQ=255)
                                   tgatgGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCa                                                                                                                <  1:65403/124‑1 (MQ=255)
                                            aaCGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCg                                                                                        >  1:449607/1‑139 (MQ=255)
                                              cGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGa                                                                                      <  1:1049750/139‑1 (MQ=255)
                                                cTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGaa                                                                                                                     >  2:294216/1‑106 (MQ=255)
                                                   gATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGccc                                                                                 <  1:756903/139‑1 (MQ=255)
                                                        ccGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATg                                                                            <  2:1168327/139‑1 (MQ=255)
                                                         cGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGc                                                                           >  1:36966/1‑139 (MQ=255)
                                                              cGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACg                                                                                                                        <  1:471554/89‑1 (MQ=255)
                                                                 tGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAg                                                                                                                   <  1:144013/91‑1 (MQ=255)
                                                                 tGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCgg                                                                                       >  1:1290/1‑119 (MQ=255)
                                                                 tGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCgg                                                                                       <  2:1290/119‑1 (MQ=255)
                                                                 tGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGaaaaa                                                                   >  1:229841/1‑139 (MQ=255)
                                                                 tGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGaaaaa                                                                   >  1:923998/1‑139 (MQ=255)
                                                                 tGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGaaaaa                                                                   >  2:1175378/1‑139 (MQ=255)
                                                                   gCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGa                                                                                      <  1:894218/118‑1 (MQ=255)
                                                                   gCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGa                                                                                      >  2:894218/1‑118 (MQ=255)
                                                                   gCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAgg                                                                 <  2:340953/139‑1 (MQ=255)
                                                                   gCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAgg                                                                 <  2:694231/139‑1 (MQ=255)
                                                                      ccGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAg                                                              <  2:449607/139‑1 (MQ=255)
                                                                         ccaccaCCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGttc                                                           >  2:569708/1‑139 (MQ=255)
                                                                          caccacCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGt                                                                                              >  1:1175490/1‑103 (MQ=255)
                                                                           accaccGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAg                                                                                                                             <  2:426531/71‑1 (MQ=255)
                                                                             caccGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGccc                                                                                 >  2:366671/1‑113 (MQ=255)
                                                                                 gTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCa                                                                                                                >  1:731275/1‑78 (MQ=255)
                                                                                 gTATCCATGCGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGGCAGTGATACCCGGAGCGACGGAAAGGCa                                                                                                                <  2:731275/78‑1 (MQ=255)
                                                                                  tATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCgg                                                                                               >  2:877564/1‑94 (MQ=255)
                                                                                  tATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCt                                                  >  1:81158/1‑139 (MQ=255)
                                                                                     ccATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGaa                                               <  2:81158/139‑1 (MQ=255)
                                                                                     ccATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGaa                                               <  2:332964/139‑1 (MQ=255)
                                                                                       aTGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACg                                             >  1:745184/1‑139 (MQ=255)
                                                                                         gggAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGcc                                           >  2:978828/1‑139 (MQ=255)
                                                                                          ggAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGc                                                                           >  1:425412/1‑106 (MQ=255)
                                                                                          ggAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCg                                          >  1:318899/1‑139 (MQ=255)
                                                                                          ggAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCACTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCg                                          <  2:467744/139‑1 (MQ=255)
                                                                                              aTGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCa                                                                                            <  2:489831/85‑1 (MQ=255)
                                                                                              aTGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCa                                                                                            >  1:489831/1‑85 (MQ=255)
                                                                                              aTGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGa                                      >  1:1000667/1‑139 (MQ=255)
                                                                                              aTGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGa                                      >  2:319767/1‑139 (MQ=255)
                                                                                               tGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACg                                                                                                                        >  2:775080/1‑56 (MQ=255)
                                                                                               tGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGttcatt                                                        >  2:1145572/1‑120 (MQ=255)
                                                                                               tGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGAt                                     >  1:410579/1‑139 (MQ=255)
                                                                                                ggCGGTGTACTGCTGGCGGAGTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGAGGGTGGAAAAAGGCAGTTTATTCACGCTGAACGCCGGTGATa                                    <  1:959256/139‑1 (MQ=255)
                                                                                                ggCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATa                                    <  1:20494/139‑1 (MQ=255)
                                                                                                ggCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATa                                    <  2:832370/139‑1 (MQ=255)
                                                                                                ggCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATa                                    <  1:1110221/139‑1 (MQ=255)
                                                                                                ggCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATa                                    <  1:347947/139‑1 (MQ=255)
                                                                                                  cGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACg                                  <  2:597917/139‑1 (MQ=255)
                                                                                                  cGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACg                                  <  2:1088061/139‑1 (MQ=255)
                                                                                                    gtgtACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAgg                                                                 >  1:1031584/1‑106 (MQ=255)
                                                                                                    gtgtACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGc                                <  2:164508/139‑1 (MQ=255)
                                                                                                     tgtACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGaa                                                                                                                     <  1:539816/53‑1 (MQ=255)
                                                                                                        aCTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACg                            <  2:617867/139‑1 (MQ=255)
                                                                                                          tgctgGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGa                          <  2:410579/139‑1 (MQ=255)
                                                                                                          tgctgGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGa                          <  2:851770/139‑1 (MQ=255)
                                                                                                          tgctgGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGa                          <  1:868203/139‑1 (MQ=255)
                                                                                                          tgctgGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGa                          <  2:540932/139‑1 (MQ=255)
                                                                                                               gCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCCTTc                                                                                                             <  2:800821/51‑1 (MQ=255)
                                                                                                               gCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAgg                                                                 <  2:1002238/95‑1 (MQ=255)
                                                                                                               gCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGcc                               <  1:280017/129‑1 (MQ=255)
                                                                                                               gCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCa                     >  2:1029558/1‑139 (MQ=255)
                                                                                                               gCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCa                     <  2:481871/139‑1 (MQ=255)
                                                                                                               gCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCa                     >  1:481871/1‑139 (MQ=255)
                                                                                                                 cGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGt                                                                                              <  2:871365/64‑1 (MQ=255)
                                                                                                                 cGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAgg                                                                                          >  1:875024/1‑68 (MQ=255)
                                                                                                                 cGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGccc                                                                                 <  2:179675/77‑1 (MQ=255)
                                                                                                                 cGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGc                                                                           <  2:1068582/83‑1 (MQ=255)
                                                                                                                 cGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAgg                                                                 <  2:126243/93‑1 (MQ=255)
                                                                                                                 cGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACg                   >  2:740773/1‑139 (MQ=255)
                                                                                                                 cGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACg                   >  1:1071765/1‑139 (MQ=255)
                                                                                                                         gTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGaaaaa                                                                   >  1:1170280/1‑83 (MQ=255)
                                                                                                                             cGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACGGTAAATGGCGGa       <  2:253590/139‑1 (MQ=255)
                                                                                                                              gCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATa                                    >  2:503440/1‑109 (MQ=255)
                                                                                                                              gCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATa                                    <  1:503440/109‑1 (MQ=255)
                                                                                                                                  tCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACGGTAAATGGCGGACTGtt  <  1:567449/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
CTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACGGTAAATGGCGGACTGTT  >  NC_000913/2072592‑2072860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: