Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,149,300	C→T	Q562Q (CAG→CAA)	yegI ←	protein kinase‑related putative non‑specific DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,149,300	0	C	T	100.0%	141.6 / NA	41	Q562Q (CAG→CAA)	yegI	protein kinase‑related putative non‑specific DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/20);  total (21/20)

CAGCCGATGTCGTTTGGCGGTCATTTTCGCCATTACCGCTTGTCTGTCTGCTGGCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAA  >  NC_000913/2149180‑2149433                                                                                                                         |                                                                                                                                      cAGCCGATGTCGTTTGGCGGTCATTTTCGCCATTACCGCTTGTCTGTCTGCTGGCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCa                                                                                                                     <  2:241365/139‑1 (MQ=255)       aTGTCGTTTGGCGGTCATTTTCGCCATTACCGCTTGTCTGTCTGCTGGCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCt                                                                                                               >  1:858684/1‑139 (MQ=255)         gTCGTTTGGCGGTCATTTTCGCCATTACCGCTTGTCTGTCTGCTGGCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCttt                                                                                                             >  1:1016046/1‑139 (MQ=255)             tttGGCGGTCATTTTCGCCATTACCGCTTGTCTGTCTGCTGGCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACt                                                                                                         >  2:883082/1‑139 (MQ=255)                 gCGGTCATTTTCGCCATTACCGCTTGTCTGTCTGCTGGCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCt                                                                                                     <  1:594582/139‑1 (MQ=255)                                  tACCGCTTGTCTGTCTGCTGGCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTa                                                                                    <  2:39553/139‑1 (MQ=255)                                              gtctgCTGGCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCt                                                                                                               >  1:1011381/1‑100 (MQ=255)                                              gtctgCTGGCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCt                                                                                                               <  2:1011381/100‑1 (MQ=255)                                                      gCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAAt                                                                <  2:1016046/139‑1 (MQ=255)                                                      gCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAAt                                                                >  1:953064/1‑139 (MQ=255)                                                         ttATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATAcc                                                             >  2:145526/1‑139 (MQ=255)                                                           aTCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCaa                                                           >  2:335394/1‑139 (MQ=255)                                                           aTCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCaa                                                           >  2:113114/1‑139 (MQ=255)                                                                 tCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGAcc                                                     >  1:535261/1‑139 (MQ=255)                                                                         ccTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAAcgcc                                             <  2:953064/139‑1 (MQ=255)                                                                             aaCACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCATTACCAAAAGACCGTAACGCCGCtt                                         >  2:657413/1‑139 (MQ=255)                                                                                  aaaaCGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCacgacg                                                                                        >  2:606892/1‑87 (MQ=255)                                                                                  aaaaCGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCacgacg                                                                                        <  1:606892/87‑1 (MQ=255)                                                                                   aaaCGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGc                                                                         >  2:647454/1‑101 (MQ=255)                                                                                   aaaCGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGc                                                                         <  1:647454/101‑1 (MQ=255)                                                                                    aaCGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACgcgc                                  <  1:568944/139‑1 (MQ=255)                                                                                       ggCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCAc                                                                                                          <  2:1069718/64‑1 (MQ=255)                                                                                       ggCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCAc                                                                                                          >  1:1069718/1‑64 (MQ=255)                                                                                       ggCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCCCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTa                                       >  1:881661/1‑131 (MQ=255)                                                                                       ggCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTa                                       <  2:881661/131‑1 (MQ=255)                                                                                       ggCGTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAgg                               >  2:477458/1‑139 (MQ=255)                                                                                          gTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCacga                                                                                          >  1:734830/1‑77 (MQ=255)                                                                                          gTTCACAGCTCGCTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCacga                                                                                          <  2:734830/77‑1 (MQ=255)                                                                                                      cTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAAt                <  1:145526/139‑1 (MQ=255)                                                                                                      cTTTCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAAt                <  1:883082/139‑1 (MQ=255)                                                                                                       tttCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTaa                                                                                                  >  2:1171594/1‑56 (MQ=255)                                                                                                       tttCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTaa                                                                                                  <  1:1171594/56‑1 (MQ=255)                                                                                                       tttCCAGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATa               <  2:858684/139‑1 (MQ=255)                                                                                                            aGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGc          >  2:298156/1‑139 (MQ=255)                                                                                                            aGTCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGc          <  2:535261/139‑1 (MQ=255)                                                                                                              tCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCa                                 <  2:136899/114‑1 (MQ=255)                                                                                                              tCGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCa                                 >  1:136899/1‑114 (MQ=255)                                                                                                               cGATAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAAc       >  1:540275/1‑139 (MQ=255)                                                                                                                 aTAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCacg                                                                                           <  2:728462/53‑1 (MQ=255)                                                                                                                 aTAACCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCacg                                                                                           >  1:728462/1‑53 (MQ=255)                                                                                                                    aCCGCTTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCaa  <  1:113114/139‑1 (MQ=255)                                                                                                                         |                                                                                                                                      CAGCCGATGTCGTTTGGCGGTCATTTTCGCCATTACCGCTTGTCTGTCTGCTGGCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAA  >  NC_000913/2149180‑2149433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑