Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,203,362	A→G	I1028M (ATA→ATG)	yehI →	DUF4132 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,203,362	0	A	G	100.0%	189.6 / NA	50	I1028M (ATA→ATG)	yehI	DUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/23);  total (27/23)

ATGCTACTCAGGTGAGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATATGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCACC  >  NC_000913/2203226‑2203495                                                                                                                                         |                                                                                                                                      aTGCTACTCAGGTGAGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAAtgtg                                                                                                                                     <  1:1110500/139‑1 (MQ=255) aTGCTACTCAGGTGAGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTCGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAAtgtg                                                                                                                                     <  1:831483/139‑1 (MQ=255)              gAGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCg                                                                                                                        >  2:777229/1‑139 (MQ=255)               aGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCgg                                                                                                                       >  2:593967/1‑139 (MQ=255)               aGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCgg                                                                                                                       >  1:45189/1‑139 (MQ=255)               aGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCgg                                                                                                                       >  2:409258/1‑139 (MQ=255)                 cAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCa                                                                                                                     >  1:449556/1‑139 (MQ=255)                    aaaCCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAAt                                                                                                                  >  2:25286/1‑139 (MQ=255)                      aCCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGa                                                                                                                >  2:185333/1‑139 (MQ=255)                          gCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACcg                                                                                                            >  1:688489/1‑139 (MQ=255)                           cTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTTCCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACcgc                                                                                                           >  1:797374/1‑139 (MQ=255)                           cTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACcgc                                                                                                           <  1:25286/139‑1 (MQ=255)                           cTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACcgc                                                                                                           >  1:1091591/1‑139 (MQ=255)                           cTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACcgc                                                                                                           <  2:45189/139‑1 (MQ=255)                                        aaaCGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCaa                                                                                              <  1:593967/139‑1 (MQ=255)                                                       ccaccCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGc                                                                               <  2:153735/139‑1 (MQ=255)                                                       ccaccCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGc                                                                               <  2:16797/139‑1 (MQ=255)                                                             gACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCaga                                                                         >  2:972524/1‑139 (MQ=255)                                                             gACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCaga                                                                         >  1:538567/1‑139 (MQ=255)                                                                 cTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     <  2:551906/139‑1 (MQ=255)                                                                 cTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     <  2:1091591/139‑1 (MQ=255)                                                                 cTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     <  1:409258/139‑1 (MQ=255)                                                                    gTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGc                                                                            >  2:67456/1‑129 (MQ=255)                                                                    gTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGc                                                                            <  1:67456/129‑1 (MQ=255)                                                                                       tctcTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATg                                               <  2:538567/139‑1 (MQ=255)                                                                                                     tcatcCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGAcctg                                 >  1:662182/1‑139 (MQ=255)                                                                                                       atcCCTTTACCCGTCTGGTTACCCAGCGATTAAtgtg                                                                                                                                     >  2:189475/1‑37 (MQ=255)                                                                                                             ttACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGa                         >  2:29114/1‑139 (MQ=255)                                                                                                              tACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGAc                        >  1:519652/1‑139 (MQ=255)                                                                                                                 ccGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGct                     <  2:743706/139‑1 (MQ=255)                                                                                                                 ccGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGct                     <  2:797374/139‑1 (MQ=255)                                                                                                                   gTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCag                                                                          <  1:518178/84‑1 (MQ=255)                                                                                                                   gTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCag                                                                          >  2:518178/1‑84 (MQ=255)                                                                                                                   gTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGctct                   >  1:962730/1‑139 (MQ=255)                                                                                                                    tCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     >  1:13153/1‑88 (MQ=255)                                                                                                                    tCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     <  2:13153/88‑1 (MQ=255)                                                                                                                    tCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTg                  >  1:491428/1‑139 (MQ=255)                                                                                                                        gTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTg              >  2:1108169/1‑139 (MQ=255)                                                                                                                         ttACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTgg             <  2:491428/139‑1 (MQ=255)                                                                                                                          tACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGct                     >  2:210549/1‑130 (MQ=255)                                                                                                                          tACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGct                     <  1:210549/130‑1 (MQ=255)                                                                                                                               aGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     <  1:385462/77‑1 (MQ=255)                                                                                                                               aGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     >  2:385462/1‑77 (MQ=255)                                                                                                                                gCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGcc      <  2:527914/139‑1 (MQ=255)                                                                                                                                gCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGcc      <  2:449556/139‑1 (MQ=255)                                                                                                                                 cGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGccc     >  2:520434/1‑139 (MQ=255)                                                                                                                                  gATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCg                                                                             >  2:741322/1‑66 (MQ=255)                                                                                                                                  gATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCg                                                                             <  1:741322/66‑1 (MQ=255)                                                                                                                                  gATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACg                       >  1:172146/1‑120 (MQ=255)                                                                                                                                  gATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACg                       <  2:172146/120‑1 (MQ=255)                                                                                                                                    ttAATGTGGGGGGTTTATCCGGCAAATGAACcgcg                                                                                                          >  1:806562/1‑35 (MQ=255)                                                                                                                                    ttAATGTGGGGGGTTTATCCGGCAAATGAACcgcg                                                                                                          <  2:806562/35‑1 (MQ=255)                                                                                                                                    ttAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCAcc  <  2:662182/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      ATGCTACTCAGGTGAGCAAAAACCTGCTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATATGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCACC  >  NC_000913/2203226‑2203495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑