Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,314,992	T→C	L502P (CTG→CCG)	rcsD →	phosphotransfer intermediate protein in two‑component regulatory system with RcsBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,314,992	0	T	C	100.0%	153.2 / NA	41	L502P (CTG→CCG)	rcsD	phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/22);  total (19/22)

AAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCAAGCGTA  >  NC_000913/2314856‑2315126                                                                                                                                         |                                                                                                                                       aaaaaCCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCgg                                                                                                                                      <  2:1121043/139‑1 (MQ=255) aaaaaCCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCgg                                                                                                                                      >  2:779591/1‑139 (MQ=255)     aCCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCg                                                                                                                                  >  1:1026581/1‑139 (MQ=255)     aCCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCg                                                                                                                                  >  2:879156/1‑139 (MQ=255)       cagcagGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGc                                                                                                                                <  1:1162339/139‑1 (MQ=255)         gcagGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTg                                                                                                                              >  2:1043686/1‑139 (MQ=255)           agGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGt                                                                                                                            <  1:812953/139‑1 (MQ=255)           agGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGt                                                                                                                            <  1:779591/139‑1 (MQ=255)           agGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGt                                                                                                                            <  2:156422/139‑1 (MQ=255)                       tCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGtta                                                                                                                          <  1:175854/129‑2 (MQ=255)                       tCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTtg                                                                                                                          >  2:175854/1‑129 (MQ=255)                       tCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACa                                                                                                                >  2:841041/1‑139 (MQ=255)                        cTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAg                                                                                                               >  2:957237/1‑139 (MQ=255)                                aaaaCATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGaa                                                                                                       <  2:1009066/139‑1 (MQ=255)                                  aaCATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACa                                                                                                     >  1:733340/1‑139 (MQ=255)                                                gcTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGgatgat                                                                                       <  1:1160346/139‑1 (MQ=255)                                                             gCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTg                                                                          <  1:715227/139‑1 (MQ=255)                                                                caGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGGACATGCTTGCGGATGATAGCTGGAAAAGTgaga                                                                       <  1:1043686/139‑1 (MQ=255)                                                                caGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga                                                                       <  1:957237/139‑1 (MQ=255)                                                                caGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga                                                                       <  2:1026581/139‑1 (MQ=255)                                                                  gTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACg                                                                     <  2:1038499/139‑1 (MQ=255)                                                                        ggCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGgatg                                                                                         >  2:589896/1‑113 (MQ=255)                                                                        ggCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGgatg                                                                                         <  1:589896/113‑1 (MQ=255)                                                                             agagCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTtctc                                                          >  2:259274/1‑139 (MQ=255)                                                                                 cgcgGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGc                                                                                                  <  2:1084461/95‑1 (MQ=255)                                                                                 cgcgGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGc                                                                                                  >  1:1084461/1‑95 (MQ=255)                                                                                 cgcgGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTg                                                      <  2:912883/139‑1 (MQ=255)                                                                                 cgcgGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTg                                                      >  1:232696/1‑139 (MQ=255)                                                                                          acacAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGACGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTa                                             <  2:948592/136‑1 (MQ=255)                                                                                                 gCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATtgatg                                      <  2:530514/139‑1 (MQ=255)                                                                                                     cGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGt                                  >  1:235347/1‑139 (MQ=255)                                                                                                                  acTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAgtg                     >  1:437109/1‑139 (MQ=255)                                                                                                                     ggCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTtg                  >  2:1000147/1‑139 (MQ=255)                                                                                                                      gCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAgtg                     >  1:258475/1‑135 (MQ=255)                                                                                                                      gCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAgtg                     <  2:258475/135‑1 (MQ=255)                                                                                                                               gCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCa        <  2:235347/139‑1 (MQ=255)                                                                                                                                cAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCaa       >  1:1052843/1‑139 (MQ=255)                                                                                                                                     gtgCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCAAGCGTa  >  1:826142/1‑139 (MQ=255)                                                                                                                                     gtgCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCAAGCGTa  <  1:316233/139‑1 (MQ=255)                                                                                                                                     gtgCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCAAGCGTa  >  1:431891/1‑139 (MQ=255)                                                                                                                                      tgCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACg                                                                     <  2:275464/71‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       AAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGTGCCTTCAGTGTTGCCTGCCATCAAGCGTA  >  NC_000913/2314856‑2315126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑