Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,388,713	T→C	L27L (TTA→CTA)	yfbO →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,388,713	0	T	C	100.0%	182.3 / NA	49	L27L (TTA→CTA)	yfbO	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/20);  total (29/20)

TGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTT  >  NC_000913/2388595‑2388848                                                                                                                       |                                                                                                                                        tGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGAttttttt                                                                                                                     >  2:301325/1‑139 (MQ=255)              atgaATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAataata                                                                                                        >  2:727357/1‑139 (MQ=255)                       ttattTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTTATACTCCTCTTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGtt                                                                                               <  1:301325/139‑1 (MQ=255)                           ttCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAat                                                                                           >  2:488691/1‑139 (MQ=255)                           ttCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAat                                                                                           >  1:883175/1‑139 (MQ=255)                           ttCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAat                                                                                           <  2:349503/139‑1 (MQ=255)                             cAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAatat                                                                                         >  1:846492/1‑139 (MQ=255)                               gggAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTATTAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGt                                                                                       >  2:110023/1‑139 (MQ=255)                                                           aTTACCCAACTTGTTAATATCAATGGTGATGTTATTAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGc                                                           <  1:110023/139‑1 (MQ=255)                                                                       gTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCtatctatc                                               >  1:1123857/1‑139 (MQ=255)                                                                                  tGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCAt                                                                                                   <  2:752073/76‑1 (MQ=255)                                                                                  tGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCAt                                                                                                   >  1:752073/1‑76 (MQ=255)                                                                                  tGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTg                                    >  1:680071/1‑139 (MQ=255)                                                                                      gATGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGaaaa                                >  2:363600/1‑139 (MQ=255)                                                                                       aTGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCg                                                            >  1:415560/1‑110 (MQ=255)                                                                                       aTGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCg                                                            <  2:415560/110‑1 (MQ=255)                                                                                       aTGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCg                                                            >  2:844188/1‑110 (MQ=255)                                                                                       aTGTTAATAATCCTGATACTCCTCGTCCATTGCGATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCg                                                            <  1:844188/110‑1 (MQ=255)                                                                                        tGTTAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATa                              >  2:292894/1‑139 (MQ=255)                                                                                          ttAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCAt                                                                                                   <  2:556652/68‑1 (MQ=255)                                                                                          ttAATAATCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCAt                                                                                                   >  1:556652/1‑68 (MQ=255)                                                                                           taataaTCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGt                           <  2:245359/139‑1 (MQ=255)                                                                                            aataatCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTg                          >  2:631448/1‑139 (MQ=255)                                                                                             ataatCCTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGa                         <  2:680071/139‑1 (MQ=255)                                                                                                  ccTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGt                           >  1:252909/1‑132 (MQ=255)                                                                                                  ccTGATACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGt                           <  2:252909/132‑1 (MQ=255)                                                                                                      aTACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCa                                                                                                    >  1:882616/1‑55 (MQ=255)                                                                                                      aTACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCa                                                                                                    <  2:882616/55‑1 (MQ=255)                                                                                                      aTACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCa                                                          >  1:61805/1‑97 (MQ=255)                                                                                                      aTACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCa                                                          <  2:61805/97‑1 (MQ=255)                                                                                                      aTACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACaa                >  2:1076715/1‑139 (MQ=255)                                                                                                       tACTCCTCGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAAt               >  2:1005144/1‑139 (MQ=255)                                                                                                            ctcGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATg                                                                                                  >  1:801136/1‑51 (MQ=255)                                                                                                            ctcGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGc                                                                                    >  2:1027602/1‑65 (MQ=255)                                                                                                            ctcGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGc                                                                                    <  1:1027602/65‑1 (MQ=255)                                                                                                            ctcGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGc                                                       >  2:537576/1‑94 (MQ=255)                                                                                                            ctcGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGc                                                       <  1:537576/94‑1 (MQ=255)                                                                                                            ctcGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAg          >  1:886273/1‑139 (MQ=255)                                                                                                            ctcGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAg          >  1:1011930/1‑139 (MQ=255)                                                                                                            ctcGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAg          >  1:558067/1‑139 (MQ=255)                                                                                                            ctcGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAg          >  1:324801/1‑139 (MQ=255)                                                                                                            ctcGTCCATTGCTATCTCTTGAGGATTTTTATATTGACAATAATATCCATg                                                                                                  <  2:801136/51‑1 (MQ=255)                                                                                                             tcGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGcta                                                     >  1:674510/1‑95 (MQ=255)                                                                                                             tcGTCCATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGcta                                                     <  2:674510/95‑1 (MQ=255)                                                                                                                  cATTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGt    <  1:292894/139‑1 (MQ=255)                                                                                                                   aTTGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGtt   <  2:558067/139‑1 (MQ=255)                                                                                                                    ttGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGc                                                       <  1:1089161/86‑1 (MQ=255)                                                                                                                    ttGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGc                                                       >  2:1139395/1‑86 (MQ=255)                                                                                                                    ttGCTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGttt  <  1:488691/139‑1 (MQ=255)                                                                                                                       |                                                                                                                                        TGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTT  >  NC_000913/2388595‑2388848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑