Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,489,435	G→A	A251V (GCA→GTA)	yfdV ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,489,435	0	G	A	100.0%	155.5 / NA	45	A251V (GCA→GTA)	yfdV	putative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/25);  total (20/25)

CTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCT  >  NC_000913/2489303‑2489557                                                                                                                                     |                                                                                                                           cTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGAcataccatac                                                                                                                      <  1:429359/139‑1 (MQ=255) cTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGAcataccatac                                                                                                                      >  2:687883/1‑139 (MQ=255)  tCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACataccatacc                                                                                                                     >  1:523057/1‑139 (MQ=255)      aGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACg                                                                                                                 <  1:1092585/139‑1 (MQ=255)                      ccAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTg                                                                                                 <  2:287787/139‑1 (MQ=255)                      ccAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTg                                                                                                 <  2:403547/139‑1 (MQ=255)                          tACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCAt                                                                                             <  2:782549/139‑1 (MQ=255)                              ggTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGa                                                                                         >  1:101200/1‑139 (MQ=255)                              ggTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGa                                                                                         >  2:811184/1‑139 (MQ=255)                               gtgtAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGaa                                                                                        <  2:101200/139‑1 (MQ=255)                                   aGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAg                                                                                    <  2:992804/139‑1 (MQ=255)                                   aGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAg                                                                                    <  1:687883/139‑1 (MQ=255)                                           aaCCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGa                                                                            <  2:349694/139‑1 (MQ=255)                                                gCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGc                                                                                                           >  1:137516/1‑103 (MQ=255)                                                gCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGc                                                                                                           <  2:137516/103‑1 (MQ=255)                                                 cTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGgtgt                                                                      >  1:1151999/1‑139 (MQ=255)                                                  tGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGtt                                                                     <  1:936655/139‑1 (MQ=255)                                                                  ggAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATc                                                                                      <  1:1058488/106‑1 (MQ=255)                                                                  ggAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATc                                                                                      >  2:1058488/1‑106 (MQ=255)                                                                  ggAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCa                                                     <  2:35896/139‑1 (MQ=255)                                                                        tGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAAc                                               >  1:127587/1‑139 (MQ=255)                                                                           cggcggTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCg                                            >  2:595039/1‑139 (MQ=255)                                                                                 tAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTa                                      <  2:263/139‑1 (MQ=255)                                                                                  aaTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTAt                                     >  2:564351/1‑139 (MQ=255)                                                                                   aTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGaa                                                                           <  1:695432/100‑1 (MQ=255)                                                                                   aTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGaa                                                                           >  2:695432/1‑100 (MQ=255)                                                                                   aTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAg                                                                          <  1:56831/101‑1 (MQ=255)                                                                                   aTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAg                                                                          >  2:56831/1‑101 (MQ=255)                                                                                          tGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGcc                             <  1:595039/139‑1 (MQ=255)                                                                                           gCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCa                            >  1:653440/1‑139 (MQ=255)                                                                                                   catcatCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAg                                                                          <  1:323443/85‑1 (MQ=255)                                                                                                   catcatCTGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAg                                                                          >  2:323443/1‑85 (MQ=255)                                                                                                          tGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGt                                                                                                  >  1:746800/1‑54 (MQ=255)                                                                                                          tGCAGATGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGt                                                                                                  <  2:746800/54‑1 (MQ=255)                                                                                                            cAGATGTTCGCTGTTCAAATGACATACCATACCAACga                                                                                                                >  1:296650/1‑38 (MQ=255)                                                                                                            cAGATGTTCGCTGTTCAAATGACATACCATACCAACga                                                                                                                <  2:296650/38‑1 (MQ=255)                                                                                                               aTGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAg                                                                          >  2:116378/1‑73 (MQ=255)                                                                                                               aTGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAg                                                                          <  1:116378/73‑1 (MQ=255)                                                                                                               aTGTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAAt        >  2:254757/1‑139 (MQ=255)                                                                                                                 gTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAAt                                                                                       <  2:1136093/58‑1 (MQ=255)                                                                                                                 gTTCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAAt                                                                                       >  1:1136093/1‑58 (MQ=255)                                                                                                                   tCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCg    <  1:254757/139‑1 (MQ=255)                                                                                                                   tCGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCg    >  2:1129701/1‑139 (MQ=255)                                                                                                                    cGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCtt                                                                                 <  2:68912/61‑1 (MQ=255)                                                                                                                    cGCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCtt                                                                                 >  1:68912/1‑61 (MQ=255)                                                                                                                     gCTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCt  <  2:653440/139‑1 (MQ=255)                                                                                                                      cTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGt                                                                                                  <  2:253331/42‑1 (MQ=255)                                                                                                                      cTGTTCAAATGACATACCATACCAACGAGAAGCAGTGCCAGt                                                                                                  >  1:253331/1‑42 (MQ=255)                                                                                                                                     |                                                                                                                           CTCACAGCCAATGACGCTGTACCAGTACGGGTGTAGACATTAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCT  >  NC_000913/2489303‑2489557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑