Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	424,284	C→T	D589D (GAC→GAT)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	424,284	0	C	T	100.0%	202.9 / NA	57	D589D (GAC→GAT)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/33);  total (24/33)

TCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAG  >  NC_000913/424151‑424416                                                                                                                                      |                                                                                                                                     tCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGAt                                                                                                                                 >  2:335618/1‑139 (MQ=255)  ccGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGAtt                                                                                                                                >  2:933951/1‑139 (MQ=255)  ccGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGAtt                                                                                                                                >  2:827552/1‑139 (MQ=255)  ccGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGAtt                                                                                                                                <  2:320130/139‑1 (MQ=255)     cgTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTg                                                                                                                             >  2:192794/1‑139 (MQ=255)                   aaCGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCAt                                                                                                               <  1:1131209/139‑1 (MQ=255)                    aCGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGAtt                                                                                                                                <  2:352996/121‑1 (MQ=255)                    aCGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGAtt                                                                                                                                >  1:352996/1‑121 (MQ=255)                    aCGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCAtt                                                                                                              <  1:260189/139‑1 (MQ=255)                         tACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGc                                                                                                         <  2:320485/139‑1 (MQ=255)                         tACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGc                                                                                                         <  1:933951/139‑1 (MQ=255)                         tACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGc                                                                                                         <  1:192794/139‑1 (MQ=255)                           cTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTa                                                                                                       <  1:335618/139‑1 (MQ=255)                              gtggCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACgg                                                                                                    <  1:1142250/139‑1 (MQ=255)                               tggCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGt                                                                                                   <  1:827552/139‑1 (MQ=255)                               tggCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGt                                                                                                   <  1:710017/139‑1 (MQ=255)                                   aaTCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGAtt                                                                                                                                <  2:802380/106‑1 (MQ=255)                                   aaTCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGAtt                                                                                                                                >  1:802380/1‑106 (MQ=255)                                   aaTCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATgg                                                                                               <  1:537615/139‑1 (MQ=255)                                                gAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGtt                                                                                  <  1:1050513/139‑1 (MQ=255)                                                    ccTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTtat                                                                              <  1:881958/139‑1 (MQ=255)                                                     cTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTtata                                                                             <  1:132656/139‑1 (MQ=255)                                                        tgAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACg                                                                          >  2:442767/1‑139 (MQ=255)                                                        tgAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACg                                                                          >  1:240257/1‑139 (MQ=255)                                                             tggtgCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTg                                                                     >  1:18872/1‑139 (MQ=255)                                                                tgCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTa                                                                                                       >  1:1093272/1‑102 (MQ=255)                                                                tgCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTa                                                                                                       <  2:1093272/102‑1 (MQ=255)                                                                tgCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGt                                                                  <  1:33002/139‑1 (MQ=255)                                                                tgCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGt                                                                  >  2:27928/1‑139 (MQ=255)                                                                     cccGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGc                                                                                                         >  2:28424/1‑95 (MQ=255)                                                                     cccGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGc                                                                                                         <  1:28424/95‑1 (MQ=255)                                                                       cgcgTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCt                                                           <  1:27928/139‑1 (MQ=255)                                                                         cgTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTa                                                                                                       >  1:573623/1‑93 (MQ=255)                                                                         cgTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTa                                                                                                       <  2:573623/93‑1 (MQ=255)                                                                                         tGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCt                                                                       <  1:365929/109‑1 (MQ=255)                                                                                         tGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCt                                                                       >  2:365929/1‑109 (MQ=255)                                                                                            cGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATaaa                                      >  1:41/1‑139 (MQ=255)                                                                                              tGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGc                                                                                                         <  2:733317/70‑1 (MQ=255)                                                                                              tGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGc                                                                                                         >  1:733317/1‑70 (MQ=255)                                                                                               gcaatggcaatgCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCca                                                     >  1:486236/1‑121 (MQ=255)                                                                                               gcaatggcaatgCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCca                                                     <  2:486236/121‑1 (MQ=255)                                                                                                     gcaatgCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTa                                                                                                       <  1:604853/65‑1 (MQ=255)                                                                                                     gcaatgCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTa                                                                                                       >  2:604853/1‑65 (MQ=255)                                                                                                        atgCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTtata                                                                             >  1:295697/1‑88 (MQ=255)                                                                                                        atgCAAAGAAGGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTtata                                                                             <  2:295697/88‑1 (MQ=255)                                                                                                             aagaagGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGcc                                                            >  2:166625/1‑100 (MQ=255)                                                                                                             aagaagGGCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGcc                                                            <  1:166625/100‑1 (MQ=255)                                                                                                                   ggCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGc                                                                                                         <  2:1011254/49‑1 (MQ=255)                                                                                                                   ggCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGc                                                                                                         >  1:1011254/1‑49 (MQ=255)                                                                                                                   ggCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAgg               <  2:298098/139‑1 (MQ=255)                                                                                                                   ggCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAgg               <  2:41/139‑1 (MQ=255)                                                                                                                   ggCATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAgg               <  2:18872/139‑1 (MQ=255)                                                                                                                     cATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGa                                                                                              <  1:1172402/58‑1 (MQ=255)                                                                                                                     cATGGGCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGa                                                                                              >  2:1172402/1‑58 (MQ=255)                                                                                                                          gCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACg                                                                                                     <  2:1034747/46‑1 (MQ=255)                                                                                                                          gCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACg                                                                                                     >  1:1034747/1‑46 (MQ=255)                                                                                                                          gCAACTGACTGATGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCAt        >  1:1058216/1‑139 (MQ=255)                                                                                                                                gactgatGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAg  <  2:240257/139‑1 (MQ=255)                                                                                                                                gactgatGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAg  <  2:1058216/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     TCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAG  >  NC_000913/424151‑424416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑