Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,007,295	T→C	V345A (GTG→GCG)	ygeW →	putative carbamoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,007,295	0	T	C	100.0%	186.8 / NA	49	V345A (GTG→GCG)	ygeW	putative carbamoyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/26);  total (23/26)

AACGCGCAACACAAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAG  >  NC_000913/3007171‑3007429                                                                                                                             |                                                                                                                                       aaCGCGCAACACAAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTAt                                                                                                                          >  1:1646/1‑139 (MQ=255) aaCGCGCAACACAAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTAt                                                                                                                          >  1:866204/1‑139 (MQ=255)           acaAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATa                                                                                                                <  1:1016015/139‑1 (MQ=255)                   tGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCg                                                                                                        <  2:1097744/139‑1 (MQ=255)                    ggCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGaa                                                                                                                     <  2:870243/125‑1 (MQ=255)                    ggCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGaa                                                                                                                     >  1:870243/1‑125 (MQ=255)                    ggCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTa                                                                                                           >  2:404839/1‑135 (MQ=255)                    ggCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTa                                                                                                           <  1:404839/135‑1 (MQ=255)                    ggCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGc                                                                                                       >  1:1067562/1‑139 (MQ=255)                    ggCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGc                                                                                                       <  2:1646/139‑1 (MQ=255)                             cTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACaa                                                                                              >  2:672910/1‑139 (MQ=255)                               gAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTa                                                                                                           >  2:92675/1‑124 (MQ=255)                               gAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTa                                                                                                           <  1:92675/124‑1 (MQ=255)                                   agaTGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGc                                                                                        <  1:429143/139‑1 (MQ=255)                                   agaTGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGc                                                                                        <  2:888335/139‑1 (MQ=255)                                     atgatgGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAgg                                                                                                                               >  2:426235/1‑98 (MQ=255)                                     atgatgGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAgg                                                                                                                               <  1:426235/98‑1 (MQ=255)                                     atgatgGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCa                                                                                      >  2:1019946/1‑139 (MQ=255)                                       gatgGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGc                                                                                    >  1:1166317/1‑139 (MQ=255)                                          ggAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTgg                                                                                 <  1:415063/139‑1 (MQ=255)                                          ggAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTgg                                                                                 <  2:866204/139‑1 (MQ=255)                                          ggAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTgg                                                                                 >  2:748747/1‑139 (MQ=255)                                               tGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGc                                                                                                       >  1:531919/1‑112 (MQ=255)                                               tGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGc                                                                                                       <  2:531919/112‑1 (MQ=255)                                                              aaGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTgg                                                                                 <  2:296590/119‑1 (MQ=255)                                                              aaGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTgg                                                                                 >  1:296590/1‑119 (MQ=255)                                                                     gTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATc                                                      <  1:672910/139‑1 (MQ=255)                                                                     gTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATc                                                      <  2:1166317/139‑1 (MQ=255)                                                                           gCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCg                                                                                                        >  1:568767/1‑83 (MQ=255)                                                                           gCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCg                                                                                                        <  2:568767/83‑1 (MQ=255)                                                                                gcctgccAGCTGATATCAGCGGCGTATCGTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAAAACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTaa                                           >  2:730764/1‑139 (MQ=255)                                                                                gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGaaaa                                                                                                                   >  2:660165/1‑67 (MQ=255)                                                                                gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGaaaa                                                                                                                   <  1:660165/67‑1 (MQ=255)                                                                                gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTgg                                                                                 >  2:1170060/1‑101 (MQ=255)                                                                                gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTgg                                                                                 <  1:1170060/101‑1 (MQ=255)                                                                                gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTaa                                           >  1:1164630/1‑139 (MQ=255)                                                                                gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTaa                                           >  2:273940/1‑139 (MQ=255)                                                                                         cTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCaa                                  <  2:1164630/139‑1 (MQ=255)                                                                                            atatCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAAcc                               <  1:955955/139‑1 (MQ=255)                                                                                                        tATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTa                                                                                                           >  1:872709/1‑51 (MQ=255)                                                                                                        tATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTa                                                                                                           <  2:872709/51‑1 (MQ=255)                                                                                                               tAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAATCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCaa            <  1:745443/139‑1 (MQ=255)                                                                                                               tAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCaa            <  1:273940/139‑1 (MQ=255)                                                                                                               tAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCaa            >  2:745443/1‑139 (MQ=255)                                                                                                                 aagaagGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAAct          <  2:1067562/139‑1 (MQ=255)                                                                                                                    aagGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgct       >  1:371700/1‑139 (MQ=255)                                                                                                                         gAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCt                   <  2:288968/122‑1 (MQ=255)                                                                                                                         gAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCt                   >  1:288968/1‑122 (MQ=255)                                                                                                                         gAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAaag  <  1:309715/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                       AACGCGCAACACAAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAG  >  NC_000913/3007171‑3007429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑