Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,447,579	G→A	A63A (GCC→GCT)	rplX ←	50S ribosomal subunit protein L24

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,447,579	0	G	A	100.0%	188.7 / NA	54	A63A (GCC→GCT)	rplX	50S ribosomal subunit protein L24
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/27);  total (27/27)

CTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGTT  >  NC_000913/3447446‑3447706                                                                                                                                      |                                                                                                                                ctCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGcttc                                                                                                                            >  1:1164895/1‑139 (MQ=255)      aaTTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCttttt                                                                                                                       >  2:241285/1‑139 (MQ=255)      aaTTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCttt                                                                                                                         >  1:681498/1‑137 (MQ=255)      aaTTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCttt                                                                                                                         <  2:681498/137‑1 (MQ=255)                  gTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCAc                                                                                                           <  1:777220/139‑1 (MQ=255)                   tttCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCAcc                                                                                                          >  1:1010675/1‑139 (MQ=255)                     tCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCg                                                                                                        >  1:954734/1‑139 (MQ=255)                     tCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCg                                                                                                        >  1:394354/1‑139 (MQ=255)                                   gaagaaACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCAc                                                                                                           <  1:657335/122‑1 (MQ=255)                                   gaagaaACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCAc                                                                                                           >  2:657335/1‑122 (MQ=255)                                   gaagaaACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGcc                                                                                          >  2:305262/1‑139 (MQ=255)                                     agaaACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCgg                                                                                        >  1:69278/1‑139 (MQ=255)                                             aCTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCt                                                                                <  2:1164895/139‑1 (MQ=255)                                                         tCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTTGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCggt                                                                                                      <  2:637544/105‑1 (MQ=255)                                                         tCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCggt                                                                                                      >  1:637544/1‑105 (MQ=255)                                                           ttCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTc                                                                     >  2:194185/1‑136 (MQ=255)                                                           ttCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTc                                                                     <  1:194185/136‑1 (MQ=255)                                                             cGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAAc                                                                <  2:394354/139‑1 (MQ=255)                                                                    aaaGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTg                                                         <  2:1010675/139‑1 (MQ=255)                                                                    aaaGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTg                                                         <  2:417670/139‑1 (MQ=255)                                                                    aaaGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTg                                                         <  2:69278/139‑1 (MQ=255)                                                                      aGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGAt                                                       <  2:672080/139‑1 (MQ=255)                                                                            cacGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGc                                                                                 <  1:619279/107‑1 (MQ=255)                                                                            cacGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGc                                                                                 >  2:619279/1‑107 (MQ=255)                                                                            cacGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTc                                                 >  1:637046/1‑139 (MQ=255)                                                                             acGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTca                                                >  2:379059/1‑139 (MQ=255)                                                                                 tCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAAcc                                                                                    >  2:505995/1‑99 (MQ=255)                                                                                 tCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAAcc                                                                                    <  1:505995/99‑1 (MQ=255)                                                                                  cAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGa                                                                                                                 >  2:528357/1‑69 (MQ=255)                                                                                  cAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGa                                                                                                                 <  1:528357/69‑1 (MQ=255)                                                                                   aGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGc                                                                                 <  1:1087129/100‑1 (MQ=255)                                                                                   aGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGc                                                                                 >  2:1087129/1‑100 (MQ=255)                                                                                       ttGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCt                                      <  2:212073/139‑1 (MQ=255)                                                                                         gCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTg                                    <  1:241285/139‑1 (MQ=255)                                                                                               tGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTg                                                         <  2:591312/112‑1 (MQ=255)                                                                                               tGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTg                                                         >  1:591312/1‑112 (MQ=255)                                                                                                                cTACGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAAc             >  1:927000/1‑139 (MQ=255)                                                                                                                   cGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGa                                                                           >  2:331843/1‑74 (MQ=255)                                                                                                                   cGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGa                                                                           <  1:331843/74‑1 (MQ=255)                                                                                                                   cGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGttt                                                                      <  1:680510/79‑1 (MQ=255)                                                                                                                   cGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGttt                                                                      >  2:680510/1‑79 (MQ=255)                                                                                                                   cGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTg                                                         <  2:1123851/92‑1 (MQ=255)                                                                                                                   cGTTGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTg                                                         >  1:1123851/1‑92 (MQ=255)                                                                                                                      tGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAAc                                                                <  1:675394/82‑1 (MQ=255)                                                                                                                      tGGAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAAc                                                                >  2:675394/1‑82 (MQ=255)                                                                                                                        gAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACGCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACcgc     >  1:501384/1‑139 (MQ=255)                                                                                                                        gAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCggt                                                                                                      <  1:197321/42‑1 (MQ=255)                                                                                                                        gAAACCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCggt                                                                                                      >  2:197321/1‑42 (MQ=255)                                                                                                                          aaCCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGt   <  2:572830/139‑1 (MQ=255)                                                                                                                           aCCTGAATAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGtt  <  1:305262/139‑1 (MQ=255)                                                                                                                                aaTAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGtt  <  2:460040/134‑1 (MQ=255)                                                                                                                                aaTAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGtt  >  1:460040/1‑134 (MQ=255)                                                                                                                                  tAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGttt                                                                      <  2:461423/64‑1 (MQ=255)                                                                                                                                  tAGCAGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGttt                                                                      >  1:461423/1‑64 (MQ=255)                                                                                                                                      |                                                                                                                                CTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCGGAACCGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGTT  >  NC_000913/3447446‑3447706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑