Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,478,883 | G→A | A575A (GCC→GCT) | kefB ← | potassium:proton antiporter |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,478,883 | 0 | G | A | 100.0% | 237.0 / NA | 67 | A575A (GCC→GCT) | kefB | potassium:proton antiporter |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (33/34); total (33/34) |
GCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCA > NC_000913/3478749‑3479016 | gCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCtt > 1:587432/1‑139 (MQ=255) gggCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTccc > 2:654015/1‑139 (MQ=255) gggCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTccc > 1:730692/1‑139 (MQ=255) tGCGCCCGCAATAAAACGTTTTCGGATTGCCGTCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCga < 2:25252/139‑1 (MQ=255) cccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGaaa > 2:623019/1‑139 (MQ=255) ccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAAt < 2:152529/139‑1 (MQ=255) cGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAAtt > 1:552078/1‑139 (MQ=255) cAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTg < 1:351213/139‑1 (MQ=255) aTAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTa < 1:597027/139‑1 (MQ=255) aTAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTa < 2:552078/139‑1 (MQ=255) aCGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTa < 2:652785/139‑1 (MQ=255) ttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc < 2:654134/139‑1 (MQ=255) ttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc < 2:587432/139‑1 (MQ=255) ttGCCGTCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGTTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGAGAGAAATTTGTACGGTATCGgcatgcat < 2:730692/139‑1 (MQ=255) ttGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgcatgcat > 1:186186/1‑139 (MQ=255) cTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATg < 2:106944/139‑1 (MQ=255) ccTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGctct > 2:1046704/1‑139 (MQ=255) tACTCAAATTCATCCCAGCCGTCGAGCTGGCGTCGCTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCgca > 1:319828/1‑139 (MQ=255) tACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCga > 1:476459/1‑96 (MQ=255) tACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCga < 2:476459/96‑1 (MQ=255) tACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTa < 1:702702/106‑1 (MQ=255) tACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGAGCGAGAAATTTGTa > 2:702702/1‑106 (MQ=255) aCTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCgcag > 2:348930/1‑139 (MQ=255) cAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCAt < 1:623019/139‑1 (MQ=255) cATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATc > 2:300557/1‑103 (MQ=255) cATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATc < 1:300557/103‑1 (MQ=255) cATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCat > 1:792707/1‑139 (MQ=255) tCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTa > 2:740195/1‑94 (MQ=255) tCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTa < 2:99443/94‑1 (MQ=255) tCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTa > 1:99443/1‑94 (MQ=255) tCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTa < 1:740195/94‑1 (MQ=255) tCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAg > 1:193788/1‑119 (MQ=255) tCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAg < 2:193788/119‑1 (MQ=255) tCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCatat > 2:101998/1‑139 (MQ=255) gCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTa < 2:928673/89‑1 (MQ=255) gCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTa > 1:928673/1‑89 (MQ=255) gCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAgg > 1:284950/1‑139 (MQ=255) gCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAgg > 2:568435/1‑139 (MQ=255) gTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAggcgg < 2:825419/139‑1 (MQ=255) tCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCTCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAggcggc > 1:676188/1‑139 (MQ=255) cAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGa < 2:479801/139‑1 (MQ=255) ggCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTa > 1:288581/1‑83 (MQ=255) ggCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTa < 2:288581/83‑1 (MQ=255) tcgtTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGt < 1:348930/139‑1 (MQ=255) ttCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc < 1:478656/81‑1 (MQ=255) ttCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc > 2:478656/1‑81 (MQ=255) ttCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTgc > 2:497203/1‑139 (MQ=255) tCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTgcg < 1:654015/139‑1 (MQ=255) gttgCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGcgctcg < 2:676188/139‑1 (MQ=255) ttgCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGcgctcgc < 2:792707/139‑1 (MQ=255) gCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc > 1:876261/1‑73 (MQ=255) gCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc < 2:876261/73‑1 (MQ=255) tttCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGa < 1:595272/116‑1 (MQ=255) tttCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGa > 2:595272/1‑116 (MQ=255) tttCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTgcg > 2:877188/1‑129 (MQ=255) tttCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTgcg < 1:877188/129‑1 (MQ=255) tCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTg < 2:24079/42‑1 (MQ=255) tCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTg > 1:24079/1‑42 (MQ=255) tCACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCt > 2:1070002/1‑139 (MQ=255) cACGCTGGAAAATCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGTAGTTGCGCTCGCTGTGCCTg > 2:71885/1‑139 (MQ=255) aaaTCTCTTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGatg < 1:1070002/139‑1 (MQ=255) tctTCCAGTTCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGatgcatgc < 2:1061574/139‑1 (MQ=255) ttCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATg < 1:1069519/96‑1 (MQ=255) ttCGCGTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATg > 2:1069519/1‑96 (MQ=255) cgcgTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGAc < 2:284950/139‑1 (MQ=255) cgcgTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGAc > 1:978020/1‑139 (MQ=255) cgTCGAGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCa > 1:792034/1‑139 (MQ=255) | GCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCA > NC_000913/3478749‑3479016 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |