Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,634,940	A→G	S34G (AGC→GGC)	yhiM →	acid resistance protein, inner membrane

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,634,940	0	A	G	100.0%	288.4 / NA	76	S34G (AGC→GGC)	yhiM	acid resistance protein, inner membrane
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/34);  total (42/34)

TATTTCAATATTAGCAGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTTACAATACATTGTTCCCCATTA  >  NC_000913/3634804‑3635075                                                                                                                                         |                                                                                                                                        tatTTCAATATTAGCAGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGc                                                                                                                                       <  1:181987/139‑1 (MQ=255)    ttCAATATTAGCAGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAg                                                                                                                                    >  2:922005/1‑139 (MQ=255)                 gggATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCgg                                                                                                                       >  1:900749/1‑139 (MQ=255)                   gATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGt                                                                                                                     >  1:466610/1‑139 (MQ=255)                         tGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCAtgtg                                                                                                               >  2:78885/1‑139 (MQ=255)                          gaggagTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGc                                                                                                              <  2:466610/139‑1 (MQ=255)                                tttAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTcgc                                                                                                                         <  1:255725/122‑1 (MQ=255)                                tttAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTcgc                                                                                                                         >  2:255725/1‑122 (MQ=255)                                tttAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGAttt                                                                                                        >  2:944482/1‑139 (MQ=255)                                  tAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGAtttat                                                                                                      >  1:443792/1‑137 (MQ=255)                                  tAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTtct                                                                                                      >  2:1174695/1‑139 (MQ=255)                                  tAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTtct                                                                                                      >  2:607103/1‑139 (MQ=255)                                  tAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTtct                                                                                                      >  2:114650/1‑139 (MQ=255)                                  tAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTGTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTtct                                                                                                      >  1:444254/1‑139 (MQ=255)                                      gTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTgg                                                                                                  <  2:44316/139‑1 (MQ=255)                                          aCATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTggcggc                                                                                              >  1:560941/1‑139 (MQ=255)                                                atatCGGGTGGCTTTTCAAATTAATCCCTTTGAGTATGTGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGTAAGGCGAGTATTTCGTCGCGGGGCATGTGCTGATTTCTCTCGCGGCCATATg                                                                                        <  1:682150/139‑1 (MQ=255)                                                 tatCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGc                                                                                       >  1:345955/1‑139 (MQ=255)                                                  atCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGcc                                                                                      <  2:560941/139‑1 (MQ=255)                                                   tCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCt                                                                                     >  2:34150/1‑139 (MQ=255)                                                   tCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCt                                                                                     >  2:539124/1‑139 (MQ=255)                                                    cGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTg                                                                                    >  2:229258/1‑139 (MQ=255)                                                     gggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTgg                                                                                   >  1:1142282/1‑139 (MQ=255)                                                     gggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTgg                                                                                   >  1:1107857/1‑139 (MQ=255)                                                      ggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGc                                                                                  >  2:426141/1‑139 (MQ=255)                                                      ggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGc                                                                                  >  2:1023141/1‑139 (MQ=255)                                                      ggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGc                                                                                  >  1:673556/1‑139 (MQ=255)                                                      ggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGc                                                                                  >  2:478474/1‑139 (MQ=255)                                                      ggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGc                                                                                  >  1:546830/1‑139 (MQ=255)                                                      ggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGc                                                                                  >  1:332661/1‑139 (MQ=255)                                                      ggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGc                                                                                  >  1:1029555/1‑139 (MQ=255)                                                      ggTGGCTTTTCAAATTAATCCCTTGGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCGGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGc                                                                                  >  2:682150/1‑139 (MQ=255)                                                       gTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCa                                                                                 >  1:228268/1‑139 (MQ=255)                                                        tGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCAtgt                                                                                                                <  1:70097/107‑1 (MQ=255)                                                        tGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCAtgt                                                                                                                >  2:70097/1‑107 (MQ=255)                                                         ggCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGTatt                                                                               >  2:759683/1‑139 (MQ=255)                                                         ggCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCa                                                                                 >  2:975381/1‑137 (MQ=255)                                                         ggCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCa                                                                                 <  1:975381/137‑1 (MQ=255)                                                          gCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCatta                                                                              >  1:904235/1‑139 (MQ=255)                                                                  aaTTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTAc                                                                      <  2:1107857/139‑1 (MQ=255)                                                                      aaTCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCa                                                                  <  2:1060387/139‑1 (MQ=255)                                                                      aaTCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCa                                                                  <  1:34150/139‑1 (MQ=255)                                                                              tGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTtattat                                                          <  1:539124/139‑1 (MQ=255)                                                                                     gggCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCg                                                   <  1:607103/139‑1 (MQ=255)                                                                                      ggCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCa                                                                                 >  2:590209/1‑108 (MQ=255)                                                                                      ggCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCa                                                                                 <  1:590209/108‑1 (MQ=255)                                                                                                gTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCAcgcg                                        <  1:1023141/139‑1 (MQ=255)                                                                                                gTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCAcgcg                                        >  2:274426/1‑139 (MQ=255)                                                                                                      cgTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGt                                  <  2:726422/139‑1 (MQ=255)                                                                                                      cgTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGt                                  <  1:1174695/139‑1 (MQ=255)                                                                                                          aGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAAt                              <  2:1029555/139‑1 (MQ=255)                                                                                                           ggTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATa                             <  1:114650/139‑1 (MQ=255)                                                                                                             tGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACg                           <  1:78885/139‑1 (MQ=255)                                                                                                             tGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACg                           <  2:505557/139‑1 (MQ=255)                                                                                                             tGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACg                           <  1:944482/139‑1 (MQ=255)                                                                                                             tGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACg                           <  1:478474/139‑1 (MQ=255)                                                                                                             tGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACg                           <  2:673556/139‑1 (MQ=255)                                                                                                               gCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGcc                                                                                             >  2:532614/1‑71 (MQ=255)                                                                                                               gCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGcc                                                                                             <  1:532614/71‑1 (MQ=255)                                                                                                               gCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGtt                         <  2:900749/139‑1 (MQ=255)                                                                                                                cTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTgg                                                                                   <  2:1100116/80‑1 (MQ=255)                                                                                                                cTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTgg                                                                                   >  1:1100116/1‑80 (MQ=255)                                                                                                                cTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGttt                        >  1:974163/1‑139 (MQ=255)                                                                                                                 tttGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGtttt                       <  2:1142282/139‑1 (MQ=255)                                                                                                                  ttGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTTa                      >  1:294717/1‑139 (MQ=255)                                                                                                                  ttGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTTa                      <  2:209180/139‑1 (MQ=255)                                                                                                                   tgtgCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGcc                                                                                      >  2:643399/1‑74 (MQ=255)                                                                                                                   tgtgCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGcc                                                                                      <  1:643399/74‑1 (MQ=255)                                                                                                                   tgtgCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTTAc                     >  1:875849/1‑139 (MQ=255)                                                                                                                     tgCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTTACaa                   >  2:141303/1‑139 (MQ=255)                                                                                                                         ggAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTTACAATACa               <  2:904235/139‑1 (MQ=255)                                                                                                                               ttCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTTACAATACATTGTTc         >  2:43592/1‑139 (MQ=255)                                                                                                                               ttCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTTACAATACATTGTTc         >  2:424997/1‑139 (MQ=255)                                                                                                                                tCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTTACAATACATTGTTcc        <  2:546830/139‑1 (MQ=255)                                                                                                                                     gCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTTACAATACATTGTTCCCCatt   <  2:228268/139‑1 (MQ=255)                                                                                                                                      cAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTTACAATACATTGTTCCCCatta  <  1:922005/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TATTTCAATATTAGCAGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTTACAATACATTGTTCCCCATTA  >  NC_000913/3634804‑3635075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑