Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,403,556	A→G	D86G (GAC→GGC)	hflC →	HflB protease modulator specific for phage lambda cII repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,403,556	0	A	G	100.0%	173.2 / NA	47	D86G (GAC→GGC)	hflC	HflB protease modulator specific for phage lambda cII repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/22);  total (25/22)

TGACAACAAACCTCTGGTTTATGAGCCGGGTCTGCATTTCAAGATACCGTTCATTGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGACCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAGTGCTGTTGAAACGTAAGTTCTCTGACCGTCTGCGTTCTG  >  NC_000913/4403422‑4403687                                                                                                                                       |                                                                                                                                    tGACAACAAACCTCTGGTTTATGAGCCGGGTCTGCATTTCAAGATACCGTTCATTGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTg                                                                                                                                 >  2:261870/1‑139 (MQ=255) tGACAACAAACCTCTGGTTTATGAGCCGGGTCTGCATTTCAAGATACCGTTCATTGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTg                                                                                                                                 <  1:410106/139‑1 (MQ=255)                             ggTCTGCATTTCAAGATACCGTTCATTGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGAtcgtcg                                                                                                                          >  2:172931/1‑118 (MQ=255)                             ggTCTGCATTTCAAGATACCGTTCATTGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGAtcgtcg                                                                                                                          <  1:172931/118‑1 (MQ=255)                                          aGATACCGTTCATTGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAg                                                                                        <  2:869701/139‑1 (MQ=255)                                             tACCGTTCATTGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCg                                                                                     >  2:972815/1‑139 (MQ=255)                                                  ttCATTGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGAtcgtc                                                                                                                           <  2:760633/96‑1 (MQ=255)                                                  ttCATTGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGAtcgtc                                                                                                                           >  1:760633/1‑96 (MQ=255)                                                  ttCATTGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTtact                                                                                >  1:1009341/1‑139 (MQ=255)                                                  ttCATTGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTtact                                                                                >  1:592445/1‑139 (MQ=255)                                                    cATTGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTtactac                                                                              <  1:9204/139‑1 (MQ=255)                                                    cATTGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTtactac                                                                              <  2:461969/139‑1 (MQ=255)                                                      ttGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCt                                                                            <  1:261870/139‑1 (MQ=255)                                                         aaaCGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGAtcgt                                                                                                                            >  1:36080/1‑88 (MQ=255)                                                         aaaCGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGAtcgt                                                                                                                            <  2:36080/88‑1 (MQ=255)                                                          aaCGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCa                                                                        >  2:832574/1‑139 (MQ=255)                                                               tGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACggg                                                                   >  1:693113/1‑139 (MQ=255)                                                               tGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACggg                                                                   >  1:645491/1‑139 (MQ=255)                                                               tGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCATCGACTCTTACATCAAATGGCGCATCAGCGATTTCa                                                                                         <  1:917876/117‑1 (MQ=255)                                                               tGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCATCGACTCTTACATCAAATGGCGCATCAGCGATTTCa                                                                                         >  2:917876/1‑117 (MQ=255)                                                                gAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGggt                                                                  >  2:569608/1‑139 (MQ=255)                                                                       cTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCg                                                           <  1:569608/139‑1 (MQ=255)                                                                               aCGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCg                                                   <  2:693113/139‑1 (MQ=255)                                                                                   aTTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAg                                               <  1:972815/139‑1 (MQ=255)                                                                                         aCCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGGCTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAg                                         >  2:629956/1‑139 (MQ=255)                                                                                         aCCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAg                                         >  1:184749/1‑139 (MQ=255)                                                                                             tGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAGTGCt                                     >  1:671098/1‑139 (MQ=255)                                                                                              ggACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGAtcgtc                                                                                                                           >  2:106887/1‑52 (MQ=255)                                                                                              ggACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGAtcgtc                                                                                                                           <  1:106887/52‑1 (MQ=255)                                                                                                   aCCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAGTGCTGTTGaa                               >  1:1112312/1‑139 (MQ=255)                                                                                                   aCCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAGTGCTGTTGaa                               >  2:497942/1‑139 (MQ=255)                                                                                                   aCCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAGTGCTGTTGaa                               >  2:572833/1‑139 (MQ=255)                                                                                                    ccAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAg                                         >  2:369168/1‑128 (MQ=255)                                                                                                    ccAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAg                                         <  1:369168/128‑1 (MQ=255)                                                                                                     cAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAg                                         <  2:140579/127‑1 (MQ=255)                                                                                                     cAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAg                                         >  1:140579/1‑127 (MQ=255)                                                                                                            aCCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAg                                                                                        >  2:206854/1‑73 (MQ=255)                                                                                                            aCCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAg                                                                                        >  2:83626/1‑73 (MQ=255)                                                                                                            aCCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAg                                                                                        <  1:206854/73‑1 (MQ=255)                                                                                                            aCCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAg                                                                                        <  1:83626/73‑1 (MQ=255)                                                                                                            aCCGCTTTGTGACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAGTGCTGTTGAAACGTAAGtt                      <  1:629956/139‑1 (MQ=255)                                                                                                                      gACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAGTGCTGTTGAAACGTAAGTTCTCTg                 <  2:784522/134‑1 (MQ=255)                                                                                                                      gACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAGTGCTGTTGAAACGTAAGTTCTCTg                 >  1:784522/1‑134 (MQ=255)                                                                                                                      gACCAAAGAGAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAGTGCTGTTGAAACGTAAGTTCTCTGACCGt            <  2:592445/139‑1 (MQ=255)                                                                                                                            agagAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAGTg                                       >  2:852611/1‑106 (MQ=255)                                                                                                                            agagAAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAGTg                                       <  1:852611/106‑1 (MQ=255)                                                                                                                             gagaAGAAAGGCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAGTGCTGTTGAAACGTAAGTTCTCTGACCGTCTGCGtt     <  2:671098/139‑1 (MQ=255)                                                                                                                                aagaaaggCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAGTGCTGTTGAAACGTAAGTTCTCTGACCGTCTGCGTTCTg  >  2:920258/1‑139 (MQ=255)                                                                                                                                    aaggCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTtactac                                                                              >  1:1179428/1‑59 (MQ=255)                                                                                                                                    aaggCCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTtactac                                                                              <  2:1179428/59‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    TGACAACAAACCTCTGGTTTATGAGCCGGGTCTGCATTTCAAGATACCGTTCATTGAAACGGTGAAAATGCTCGACGCACGTATTCAGACCATGGACAACCAGGCCGACCGCTTTGTGACCAAAGAGAAGAAAGACCTGATCGTCGACTCTTACATCAAATGGCGCATCAGCGATTTCAGCCGTTACTACCTGGCAACGGGTGGTGGCGACATTTCGCAAGCGGAAGTGCTGTTGAAACGTAAGTTCTCTGACCGTCTGCGTTCTG  >  NC_000913/4403422‑4403687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑