Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,431,613	T→C	E124G (GAA→GGA)	ytfE ←	iron‑sulfur cluster repair protein RIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,431,613	0	T	C	100.0%	211.6 / NA	55	E124G (GAA→GGA)	ytfE	iron‑sulfur cluster repair protein RIC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

CTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCA  >  NC_000913/4431479‑4431742                                                                                                                                       |                                                                                                                                  cTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATg                                                                                                                               >  2:811974/1‑139 (MQ=255)      agcagTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATgcagca                                                                                                                          <  2:963975/139‑1 (MQ=255)            tCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGt                                                                                                                      >  2:413995/1‑137 (MQ=255)            tCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGt                                                                                                                      <  1:413995/137‑1 (MQ=255)            tCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCa                                                                                                                    <  2:908826/139‑1 (MQ=255)             cGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAg                                                                                                                   <  2:424461/139‑1 (MQ=255)                                 cTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTg                                                                                               >  2:1133610/1‑139 (MQ=255)                                          aCGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCg                                                                                      >  1:1034035/1‑139 (MQ=255)                                              tGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGttt                                                                                  >  2:1098724/1‑139 (MQ=255)                                                         aTTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGaa                                                                       <  1:1133610/139‑1 (MQ=255)                                                          ttGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATgca                                                                                                                             >  2:635849/1‑84 (MQ=255)                                                          ttGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATgca                                                                                                                             <  1:635849/84‑1 (MQ=255)                                                          ttGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTc                                                                                                         <  2:1034551/104‑1 (MQ=255)                                                          ttGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGAGCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTc                                                                                                         >  1:1034551/1‑104 (MQ=255)                                                              cTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACgcgc                                                                  <  1:127000/139‑1 (MQ=255)                                                              cTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACgcgc                                                                  <  2:363617/139‑1 (MQ=255)                                                                         tGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGt                                                       <  1:577083/139‑1 (MQ=255)                                                                           ccTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCg                                                     <  2:815396/139‑1 (MQ=255)                                                                               gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATg                                                                                                                               >  1:199890/1‑61 (MQ=255)                                                                               gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATg                                                                                                                               <  2:199890/61‑1 (MQ=255)                                                                               gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTgg                                                                                              >  1:1157912/1‑94 (MQ=255)                                                                               gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTgg                                                                                              <  2:1157912/94‑1 (MQ=255)                                                                               gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCt                                                                                        >  1:257470/1‑100 (MQ=255)                                                                               gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCt                                                                                        <  2:257470/100‑1 (MQ=255)                                                                               gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTg                                                 >  2:805005/1‑139 (MQ=255)                                                                               gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTg                                                 >  2:319704/1‑139 (MQ=255)                                                                                       atcGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCa                                         <  2:1034035/139‑1 (MQ=255)                                                                                         cGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATgcagca                                                                                                                          <  2:1113299/56‑1 (MQ=255)                                                                                         cGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATgcagca                                                                                                                          >  1:1113299/1‑56 (MQ=255)                                                                                         cGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCGGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGc                                       <  1:805005/139‑1 (MQ=255)                                                                                          gggAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTgg                                                                                              <  1:1082716/83‑1 (MQ=255)                                                                                          gggAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTgg                                                                                              >  2:1082716/1‑83 (MQ=255)                                                                                                   aTCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGtt                             >  2:1078924/1‑139 (MQ=255)                                                                                                       gCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAg                                                                                                       >  1:176588/1‑61 (MQ=255)                                                                                                       gCTCTTCTTTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAg                                                                                                       <  2:176588/61‑1 (MQ=255)                                                                                                          cttcttTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGt                                                       >  2:1159636/1‑106 (MQ=255)                                                                                                          cttcttTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGt                                                       <  1:1159636/106‑1 (MQ=255)                                                                                                          cttcttTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTcgcg                      >  2:256217/1‑139 (MQ=255)                                                                                                           ttcttTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCgg                     >  1:272865/1‑139 (MQ=255)                                                                                                             cttTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTg                   <  2:272865/139‑1 (MQ=255)                                                                                                             cttTCATCATGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTg                   <  1:811974/139‑1 (MQ=255)                                                                                                                  atcatGTGGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGAt              <  1:256217/139‑1 (MQ=255)                                                                                                                       gtgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCt                                                                                        >  1:773658/1‑60 (MQ=255)                                                                                                                       gtgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCt                                                                                        <  2:773658/60‑1 (MQ=255)                                                                                                                       gtgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTg                   >  2:559269/1‑129 (MQ=255)                                                                                                                       gtgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTg                   <  1:559269/129‑1 (MQ=255)                                                                                                                       gtgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTgg         >  2:416655/1‑139 (MQ=255)                                                                                                                       gtgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTgg         >  1:464700/1‑139 (MQ=255)                                                                                                                       gtgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTgg         >  2:1102827/1‑139 (MQ=255)                                                                                                                        tgGCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGt        >  2:906537/1‑139 (MQ=255)                                                                                                                          gCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGAc                 >  1:1168251/1‑128 (MQ=255)                                                                                                                          gCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGAc                 <  2:1168251/128‑1 (MQ=255)                                                                                                                          gCTGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAg      >  2:764541/1‑139 (MQ=255)                                                                                                                            tGGAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAgcg    >  1:517369/1‑139 (MQ=255)                                                                                                                              gAAAGCTCTCCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCa  <  1:1102827/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  CTTCCAGCAGTTCGCCCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCA  >  NC_000913/4431479‑4431742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑