Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,513,332	C→T	L26L (CTG→CTA)	fecC ←	ferric citrate ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,513,332	0	C	T	100.0%	104.0 / NA	31	L26L (CTG→CTA)	fecC	ferric citrate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/18);  total (13/18)

CGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCA  >  NC_000913/4513199‑4513460                                                                                                                                      |                                                                                                                                 cGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACt                                                                                                                             <  2:706354/139‑1 (MQ=255)       gAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCa                                                                                                                       <  2:1139993/139‑1 (MQ=255)         aCGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGa                                                                                                                     <  1:772961/139‑1 (MQ=255)                   ggCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAaataataat                                                                                                           <  1:1170193/139‑1 (MQ=255)                        cGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGtg                                                                                                      <  2:1028274/139‑1 (MQ=255)                          tGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCa                                                                                                                       >  2:1064301/1‑120 (MQ=255)                          tGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCa                                                                                                                       <  1:1064301/120‑1 (MQ=255)                                         ttGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGcccc                                                                                     <  2:865966/139‑1 (MQ=255)                                                aGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGcccc                                                                                     >  1:403377/1‑132 (MQ=255)                                                aGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGcccc                                                                                     <  2:403377/132‑1 (MQ=255)                                                    cTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACgg                                                                                             >  1:179257/1‑120 (MQ=255)                                                    cTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACgg                                                                                             <  2:179257/120‑1 (MQ=255)                                                               gTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTa                                                               >  2:738475/1‑139 (MQ=255)                                                                    cGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATcgcg                                                          >  2:825217/1‑139 (MQ=255)                                                                                  gcagGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACg                                            >  2:895609/1‑139 (MQ=255)                                                                                  gcagGGCGCGGGTTGCAGCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATACTAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACg                                            >  1:674123/1‑139 (MQ=255)                                                                                   cagGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGaaaa                                                                                                                  <  2:408144/68‑1 (MQ=255)                                                                                   cagGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGaaaa                                                                                                                  >  1:408144/1‑68 (MQ=255)                                                                                    agGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGt                                          <  2:462967/139‑1 (MQ=255)                                                                                      ggCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTaa                                        <  1:825217/139‑1 (MQ=255)                                                                                        cgcgGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGaaa                                                                                                                   <  2:1174054/62‑1 (MQ=255)                                                                                        cgcgGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGaaa                                                                                                                   >  1:1174054/1‑62 (MQ=255)                                                                                                 cATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGAtgg                             >  2:5856/1‑139 (MQ=255)                                                                                                                cAGGAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTAcc              <  1:5856/139‑1 (MQ=255)                                                                                                                   gAATGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCgtg           >  2:999662/1‑139 (MQ=255)                                                                                                                     aTGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTc         <  1:895609/139‑1 (MQ=255)                                                                                                                      tGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTg                                 >  2:153907/1‑114 (MQ=255)                                                                                                                      tGGCCGAGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTg                                 <  1:153907/114‑1 (MQ=255)                                                                                                                            aGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCa  <  2:217413/139‑1 (MQ=255)                                                                                                                            aGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCa  >  2:642558/1‑139 (MQ=255)                                                                                                                            aGTAGCAAAATAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCa  <  2:674123/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                 CGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCA  >  NC_000913/4513199‑4513460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑