Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,538,491 C→T G92D (GGC→GAC)  nanM ← N‑acetylneuraminic acid mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,538,4910CT100.0% 233.9 / NA 67G92D (GGC→GAC) nanMN‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/30);  total (37/30)

TTGTGTACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGCCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCTTGTACCATGC  >  NC_000913/4538356‑4538615
                                                                                                                                       |                                                                                                                            
ttGTGTACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATgtcgc                                                                                                                           <  2:762100/139‑1 (MQ=255)
    gtACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCaaa                                                                                                                       <  2:624166/139‑1 (MQ=255)
        aaaaGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAAcaca                                                                                                                   <  1:1010687/139‑1 (MQ=255)
             tCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATgtcgc                                                                                                                           >  1:562300/1‑126 (MQ=255)
             tCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATgtcgc                                                                                                                           <  2:562300/126‑1 (MQ=255)
               acaTGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGAt                                                                                                            >  2:76496/1‑139 (MQ=255)
                  tGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGc                                                                                                         <  2:360124/139‑1 (MQ=255)
                    gCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCa                                                                                                       <  1:76496/139‑1 (MQ=255)
                    gCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCa                                                                                                       <  2:63881/139‑1 (MQ=255)
                         ccATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAAt                                                                                                  <  2:61157/139‑1 (MQ=255)
                              cccATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATg                                                                                             >  1:654501/1‑139 (MQ=255)
                                   cGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCagag                                                                                        >  1:352471/1‑139 (MQ=255)
                                   cGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCagag                                                                                        <  2:399912/139‑1 (MQ=255)
                                   cGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCagag                                                                                        <  2:759435/139‑1 (MQ=255)
                                      cgcgTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGtt                                                                                     >  2:1136231/1‑139 (MQ=255)
                                        cgTGTGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGc                                                                                   >  1:996824/1‑139 (MQ=255)
                                        cgTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGc                                                                                   >  2:847834/1‑139 (MQ=255)
                                         gTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCt                                                                                  <  1:847834/139‑1 (MQ=255)
                                           gtgACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTg                                                                                <  2:996824/137‑1 (MQ=255)
                                            cGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGa                                                                               >  1:434519/1‑139 (MQ=255)
                                                aTCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGAtctc                                                                           >  1:237778/1‑139 (MQ=255)
                                                             aaCTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAgg                                                              >  2:51008/1‑139 (MQ=255)
                                                             aaCTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAgg                                                              <  2:1069314/139‑1 (MQ=255)
                                                              aCTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATgtcgc                                                                                                                           <  2:1151784/77‑1 (MQ=255)
                                                              aCTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATgtcgc                                                                                                                           >  1:1151784/1‑77 (MQ=255)
                                                              aCTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAggg                                                             >  2:464058/1‑139 (MQ=255)
                                                               cTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCa                                                                                                       >  2:165888/1‑96 (MQ=255)
                                                               cTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCa                                                                                                       <  1:165888/96‑1 (MQ=255)
                                                                       tttGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAAt                                                                                                  <  2:1091203/93‑1 (MQ=255)
                                                                       tttGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAAt                                                                                                  >  1:1091203/1‑93 (MQ=255)
                                                                         tGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCt                                                                                  >  1:160301/1‑107 (MQ=255)
                                                                         tGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCt                                                                                  <  2:160301/107‑1 (MQ=255)
                                                                         tGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTaa                                                  >  2:459670/1‑139 (MQ=255)
                                                                         tGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTaa                                                  >  1:929892/1‑139 (MQ=255)
                                                                         tGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTaa                                                  >  1:1154620/1‑139 (MQ=255)
                                                                               tGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGt                                            >  1:21968/1‑139 (MQ=255)
                                                                               tGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGt                                            >  1:607271/1‑139 (MQ=255)
                                                                                         tACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCaga                                                                                         <  1:285355/84‑1 (MQ=255)
                                                                                         tACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCaga                                                                                         >  2:285355/1‑84 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCaaa                                                                                                                       <  1:617030/52‑1 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCaaa                                                                                                                       >  2:617030/1‑52 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAAcaca                                                                                                                   <  2:850448/56‑1 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAAcaca                                                                                                                   >  1:850448/1‑56 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTg                                                                                >  1:145457/1‑91 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTg                                                                                <  2:145457/91‑1 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATc                                >  1:520701/1‑139 (MQ=255)
                                                                                                 tAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGc                          >  2:1115686/1‑139 (MQ=255)
                                                                                                  aaaTACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGcc                         <  1:1136231/139‑1 (MQ=255)
                                                                                                        cTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTa                   >  2:667971/1‑139 (MQ=255)
                                                                                                        cTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTa                   >  1:556411/1‑139 (MQ=255)
                                                                                                          gAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCa                                                                                                                         >  2:917258/1‑35 (MQ=255)
                                                                                                          gAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCa                                                                                                                         <  1:917258/35‑1 (MQ=255)
                                                                                                           aGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCaaa                                                                                                                       >  1:247062/1‑36 (MQ=255)
                                                                                                           aGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCaaa                                                                                                                       <  2:247062/36‑1 (MQ=255)
                                                                                                           aGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATcc                >  1:649169/1‑139 (MQ=255)
                                                                                                             tCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCa                                                                                                       >  1:714126/1‑50 (MQ=255)
                                                                                                             tCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCa                                                                                                       <  2:714126/50‑1 (MQ=255)
                                                                                                             tCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTg                                             <  1:115500/108‑1 (MQ=255)
                                                                                                             tCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTg                                             >  2:115500/1‑108 (MQ=255)
                                                                                                               aaGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTTCCATc                                                                                                     >  2:299148/1‑50 (MQ=255)
                                                                                                               aaGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATc                                                                                                     <  1:299148/50‑1 (MQ=255)
                                                                                                               aaGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCt            >  2:970104/1‑139 (MQ=255)
                                                                                                                  cccTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACg                                                <  1:633699/100‑1 (MQ=255)
                                                                                                                  cccTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCTTGt         >  2:71858/1‑139 (MQ=255)
                                                                                                                  cccTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGATTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACg                                                >  2:633699/1‑100 (MQ=255)
                                                                                                                     tCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCa                                                                                           <  2:394800/54‑1 (MQ=255)
                                                                                                                     tCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCa                                                                                           >  1:394800/1‑54 (MQ=255)
                                                                                                                      cGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTgtc                                                                                                        <  2:666860/40‑3 (MQ=255)
                                                                                                                      cGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTgtc                                                                                                        >  1:666860/1‑38 (MQ=255)
                                                                                                                      cGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCTTGTACCa     <  1:31543/139‑1 (MQ=255)
                                                                                                                         tGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCTTGTACCATGc  <  2:434519/139‑1 (MQ=255)
                                                                                                                         tGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCTTGTACCATGc  <  2:929892/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                            
TTGTGTACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGCCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCTTGTACCATGC  >  NC_000913/4538356‑4538615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑