Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,544,191	A→G	K155R (AAA→AGA)	fimI →	fimbrial protein involved in type 1 pilus biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,544,191	0	A	G	100.0%	255.5 / NA	67	K155R (AAA→AGA)	fimI	fimbrial protein involved in type 1 pilus biosynthesis
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/39);  total (28/39)

GCCAGGGATAGCCACCAATATTGGCGTAGCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAAATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGT  >  NC_000913/4544063‑4544320                                                                                                                                 |                                                                                                                                  gCCAGGGATAGCCACCAATATTGGCGTAGCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCAAGATATCGTGCt                                                                                                                         >  1:574624/1‑139 (MQ=255)  ccAGGGATAGCCACCAATATTGGCGTAGCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTa                                                                                                                        >  1:102563/1‑139 (MQ=255)           gCCACCAATATTGGCGTAGCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTc                                                                                                               >  2:997237/1‑139 (MQ=255)           gCCACCAATATTAGCGTAGCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTc                                                                                                               >  2:348423/1‑139 (MQ=255)                      tGGCGTAGCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTggc                                                                                                    >  2:503668/1‑139 (MQ=255)                         cGTAGCGTTGTTTGATGATGAAGGAACCCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTggcggc                                                                                                 <  2:697709/139‑1 (MQ=255)                          gTAGCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCa                                                                                                <  2:768807/139‑1 (MQ=255)                             gCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGata                                                                                                                                <  2:846356/104‑1 (MQ=255)                             gCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTATACTTCGCTACATTTCATCGCCAGata                                                                                                                                >  1:846356/1‑104 (MQ=255)                                ttgttTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCa                                                                                          <  1:72921/139‑1 (MQ=255)                                   tttGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCa                                                                                                <  2:697932/130‑1 (MQ=255)                                   tttGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCa                                                                                                >  1:697932/1‑130 (MQ=255)                                        tgatgaAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCa                                                                                                <  2:632017/125‑1 (MQ=255)                                        tgatgaAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCa                                                                                                >  1:632017/1‑125 (MQ=255)                                               ggAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGt                                                                           <  2:102563/139‑1 (MQ=255)                                                gAAGCCTCGTACCGATTAATCGTCCTCCAGCAAACTGGGAGCGGCTTTATTCAGGCTCTACGTCGCTACATTTCGTCGCCAGATGTGGGGGGACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGtt                                                                          <  1:1001993/139‑1 (MQ=255)                                                         tACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCAt                                                                                               >  2:547471/1‑109 (MQ=255)                                                         tACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCAt                                                                                               <  1:547471/109‑1 (MQ=255)                                                          aCCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGcc                                                                                     >  1:783699/1‑118 (MQ=255)                                                          aCCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGcc                                                                                     <  2:783699/118‑1 (MQ=255)                                                              aTTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCg                                                                                                                 >  2:988108/1‑86 (MQ=255)                                                              aTTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCg                                                                                                                 <  1:988108/86‑1 (MQ=255)                                                              aTTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCa                                                                                          <  1:794395/109‑1 (MQ=255)                                                              aTTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCa                                                                                          >  2:794395/1‑109 (MQ=255)                                                              aTTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATc                                                            >  1:1035379/1‑139 (MQ=255)                                                               ttAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCa                                                           >  1:581495/1‑139 (MQ=255)                                                                   tCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTaa                                                       <  2:587968/139‑1 (MQ=255)                                                                     gTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCAGCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAAtt                                                     <  1:1124820/139‑1 (MQ=255)                                                                        ctccAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTaa                                                                  >  1:603780/1‑123 (MQ=255)                                                                        ctccAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTaa                                                                  <  2:603780/123‑1 (MQ=255)                                                                        ctccAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGtt                                                  <  2:581495/139‑1 (MQ=255)                                                                           cAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCa                                                                                          >  1:1044491/1‑96 (MQ=255)                                                                           cAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCa                                                                                          <  2:1044491/96‑1 (MQ=255)                                                                                agcTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGAt                                          <  2:574624/137‑1 (MQ=255)                                                                                aaCTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTgg                                                                            >  2:977380/1‑105 (MQ=255)                                                                                aaCTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTgg                                                                            <  1:977380/105‑1 (MQ=255)                                                                                aaCTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGAt                                          <  2:1035379/139‑1 (MQ=255)                                                                                 aCTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATa                                         >  2:1004610/1‑139 (MQ=255)                                                                                   tGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCCATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAAt                                       <  2:732804/139‑1 (MQ=255)                                                                                      aaaCGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAAtgtg                                    <  1:670125/139‑1 (MQ=255)                                                                                        aCGGCTTTATTCATGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGAt                                  <  2:680489/139‑1 (MQ=255)                                                                                           gCTTTATTGAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATaac                               <  1:715628/139‑1 (MQ=255)                                                                                           gCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATaac                               <  1:1004610/139‑1 (MQ=255)                                                                                           gCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATaac                               <  1:348423/139‑1 (MQ=255)                                                                                                aTTCAGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATaacaggaa                          <  1:503668/139‑1 (MQ=255)                                                                                                    aGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGAt                                          >  2:783623/1‑119 (MQ=255)                                                                                                    aGGCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGAt                                          <  1:783623/119‑1 (MQ=255)                                                                                                     ggCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAacaggaacagga                     <  2:187479/139‑1 (MQ=255)                                                                                                      gCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCa                                                                                          <  2:654489/69‑1 (MQ=255)                                                                                                      gCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGt                                                                           <  2:1085953/84‑1 (MQ=255)                                                                                                      gCTCTACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGt                                                                           >  1:1085953/1‑84 (MQ=255)                                                                                                      gCTCTACTTCGCTACATTGCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCa                                                                                          >  1:654489/1‑69 (MQ=255)                                                                                                         ctACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGcc                                                                                     <  2:442172/71‑1 (MQ=255)                                                                                                         ctACTTCGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGcc                                                                                     >  1:442172/1‑71 (MQ=255)                                                                                                               cGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCa                                                                                          <  1:277245/60‑1 (MQ=255)                                                                                                               cGCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCa                                                                                          >  2:277245/1‑60 (MQ=255)                                                                                                                gCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGt                                                         >  2:1026953/1‑92 (MQ=255)                                                                                                                gCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGt                                                         <  1:1026953/92‑1 (MQ=255)                                                                                                                gCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAAtgt                                     >  1:776256/1‑112 (MQ=255)                                                                                                                gCTACATTTCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAAtgt                                     <  2:776256/112‑1 (MQ=255)                                                                                                                      tttCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACa                   <  1:445153/124‑1 (MQ=255)                                                                                                                      tttCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACa                   >  2:445153/1‑124 (MQ=255)                                                                                                                       ttCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCGGCAGATAATGTGATaac                               <  1:673064/111‑1 (MQ=255)                                                                                                                       ttCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATaac                               >  2:673064/1‑111 (MQ=255)                                                                                                                        tCATCGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGt  <  2:987345/139‑1 (MQ=255)                                                                                                                            cGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGt                                                                           <  1:277031/62‑1 (MQ=255)                                                                                                                            cGCCAGATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGt                                                                           >  2:277031/1‑62 (MQ=255)                                                                                                                                 |                                                                                                                                  GCCAGGGATAGCCACCAATATTGGCGTAGCGTTGTTTGATGATGAAGGAAACCTCGTACCGATTAATCGTCCTCCAGCAAACTGGAAACGGCTTTATTCAGGCTCTACTTCGCTACATTTCATCGCCAAATATCGTGCTACCGGGCGTCGGGTTACTGGCGGCATCGCCAATGCCCAGGCCTGGTTCTCTTTAACCTATCAGTAATTGTTCAGCAGATAATGTGATAACAGGAACAGGACAGTGAGTAATAAAAACGT  >  NC_000913/4544063‑4544320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑