Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,585,984	C→T	A260T (GCC→ACC)	hsdR ←	endonuclease R Type I restriction enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,585,984	0	C	T	100.0%	147.1 / NA	43	A260T (GCC→ACC)	hsdR	endonuclease R Type I restriction enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/27);  total (16/27)

GGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTAAGCCTTACGTTCC  >  NC_000913/4585849‑4586102                                                                                                                                        |                                                                                                                       ggCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTg                                                                                                                     <  1:849961/139‑1 (MQ=255)      aCAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGc                                                                                                                <  2:941180/139‑1 (MQ=255)      aCAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGc                                                                                                                <  1:624824/139‑1 (MQ=255)      aCAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGC‑TTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTg                                                                                                                     >  1:947101/1‑133 (MQ=255)      aCAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGC‑TTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTg                                                                                                                     <  2:947101/133‑1 (MQ=255)           cAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCt                                                                                                           <  2:932452/139‑1 (MQ=255)                caTAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACg                                                                                                      <  2:467386/139‑1 (MQ=255)                caTAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACg                                                                                                      <  1:451591/139‑1 (MQ=255)                        gCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTgcg                                                                                              >  1:70280/1‑139 (MQ=255)                               ccTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCatcaat                                                                                       <  2:109891/139‑1 (MQ=255)                                 tGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCatcaatca                                                                                     >  2:1148487/1‑139 (MQ=255)                                 tGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCatcaatca                                                                                     >  1:10015/1‑139 (MQ=255)                                    ttACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGa                                                                                  <  2:70280/139‑1 (MQ=255)                                           tttCATCTTTTCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGAct                                                                           <  2:908406/139‑1 (MQ=255)                                                 cTTTTCCGGTCGGCCATTCGGCAATGGCTTTATCGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCtcttc                                                                     <  2:10015/139‑1 (MQ=255)                                                    ttCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGc                                                                                                                <  2:977348/93‑1 (MQ=255)                                                    ttCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGc                                                                                                                >  1:977348/1‑93 (MQ=255)                                                     tCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCg                                                                 >  1:468579/1‑139 (MQ=255)                                                     tCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCg                                                                 <  1:885445/139‑1 (MQ=255)                                                     tCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCg                                                                 <  2:177772/139‑1 (MQ=255)                                                       cGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCt                                                               <  1:1114696/139‑1 (MQ=255)                                                       cGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCt                                                               <  1:514578/139‑1 (MQ=255)                                                             gCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGtt                                                         <  1:195721/139‑1 (MQ=255)                                                             gCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCACCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGtt                                                         <  2:1017847/139‑1 (MQ=255)                                                                           gCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGa                                           >  2:472933/1‑139 (MQ=255)                                                                             tttATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATg                                         <  1:46627/139‑1 (MQ=255)                                                                               tATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGc                                       <  2:870289/139‑1 (MQ=255)                                                                                     cGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATc                                 >  2:381871/1‑139 (MQ=255)                                                                                      gCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCg                                                                 >  2:295688/1‑106 (MQ=255)                                                                                      gCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCg                                                                 <  1:295688/106‑1 (MQ=255)                                                                                      gCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCt                                                               >  2:453877/1‑108 (MQ=255)                                                                                      gCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCt                                                               <  1:453877/108‑1 (MQ=255)                                                                                      gCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGa                                   >  1:544690/1‑136 (MQ=255)                                                                                      gCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGa                                   <  2:544690/136‑1 (MQ=255)                                                                                          ggTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTaa                            <  1:1148487/139‑1 (MQ=255)                                                                                                      gcgcCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAgt                                                     >  1:884751/1‑102 (MQ=255)                                                                                                      gcgcCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGt                >  2:824793/1‑139 (MQ=255)                                                                                                      gcgcCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGt                <  2:518949/139‑1 (MQ=255)                                                                                                      gcgcCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGt                >  2:197682/1‑139 (MQ=255)                                                                                                      gcgcCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTGGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAgt                                                     <  2:884751/102‑1 (MQ=255)                                                                                                                    gcgcAGGGTTTTGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTAAGCCTTACGTTcc  >  2:715222/1‑139 (MQ=255)                                                                                                                               tGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCat                                                                                           >  1:1168991/1‑39 (MQ=25)                                                                                                                               tGCTGTCGGTCTGCCAGCCTGCTTTACGCAGTTGCGCat                                                                                           <  2:1168991/39‑1 (MQ=25)                                                                                                                                        |                                                                                                                       GGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCGGCAATGGCTTTATTGACGCCGGGTTCCGGACGTGCGCCTTTGGAGAAGCGCAGGGTTTTGCTGTCGGCCTGCCAGCCTGCTTTACGCAGTTGCGCATCAATCAGGAAGCGACTCTCTTCTTCGCTAAGGTTGAGTGTGCGCTTGATGGCCTGATCGGTAATTTCTTTGTGGTAAGCCTTACGTTCC  >  NC_000913/4585849‑4586102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑