Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 3,970,427 (ACG)4→3 coding (295‑297/1131 nt) wecB → UDP‑N‑acetyl glucosamine‑2‑epimerase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3970418 3970420 3 62 [0] [0] 62 wecB UDP‑N‑acetyl glucosamine‑2‑epimerase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3970421 =0 (0.000)62 (1.250) 44/218 0.1 100% coding (289/1131 nt) wecB UDP‑N‑acetyl glucosamine‑2‑epimerase
?NC_000913 = 3970417 0 (0.000)coding (285/1131 nt) wecB UDP‑N‑acetyl glucosamine‑2‑epimerase

CGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTACATTGACCGGGCATCTG  >  NC_000913/3970300‑3970555
                                                                                                                     |                                                                                                                                          
cGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagc                                                                                                                       <  2:307524‑M2/139‑22 (MQ=255)
   tctcAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctg                                                                                                                    <  2:477589‑M2/139‑25 (MQ=255)
     tcAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggc                                                                                                                  >  1:650594‑M2/1‑113 (MQ=255)
     tcAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggc                                                                                                                  <  2:355986‑M2/139‑27 (MQ=255)
      cAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcg                                                                                                                 <  1:407476‑M2/139‑28 (MQ=255)
       aaCATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcgg                                                                                                                <  2:183830‑M2/139‑29 (MQ=255)
          aTAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgt                                                                                                             <  1:1045053‑M2/139‑32 (MQ=255)
            aaTGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttt                                                                                                           <  1:388637‑M2/139‑34 (MQ=255)
              tGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgtttta                                                                                                         <  2:105916‑M2/139‑36 (MQ=255)
              tGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgtttta                                                                                                         <  1:873190‑M2/139‑36 (MQ=255)
              tGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggc                                                                                                                  <  2:879258‑M2/130‑27 (MQ=255)
              tGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggc                                                                                                                  >  1:879258‑M2/1‑104 (MQ=255)
              tGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaac                                                                                                                           <  2:267487‑M2/121‑18 (MQ=255)
              tGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaac                                                                                                                           >  1:267487‑M2/1‑104 (MQ=255)
                 aGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatca                                                                                                      <  2:1018593‑M2/139‑39 (MQ=255)
                 aGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatca                                                                                                      <  1:121253‑M2/139‑39 (MQ=255)
                 aGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggc                                                                                                                  >  2:898292‑M2/1‑101 (MQ=255)
                 aGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggc                                                                                                                  <  1:898292‑M2/127‑27 (MQ=255)
                 aGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggc                                                                                                                  <  1:697754‑M2/127‑27 (MQ=255)
                 aGCCAGGACAGGGCCTGACAGAGACAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggc                                                                                                                  >  2:697754‑M2/1‑101 (MQ=255)
                   ccAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagc                                                                                                    <  2:383360‑M2/139‑41 (MQ=255)
                    cAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcg                                                                                                   <  1:1031481‑M2/139‑42 (MQ=255)
                       gACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtat                                                                                                <  1:217094‑M2/139‑45 (MQ=255)
                                 gACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggt                                                                                      >  1:1104684‑M2/1‑85 (MQ=255)
                                  aCAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtc                                                                                     >  2:497726‑M2/1‑84 (MQ=255)
                                      agaTAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattccagttggtcacgt                                                                                 >  2:1031481‑M2/1‑80 (MQ=255)
                                                tCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggt                                                                       <  2:88165‑M2/139‑70 (MQ=255)
                                                tCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggt                                                                       >  2:1045053‑M2/1‑70 (MQ=255)
                                                tCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttat                                                                                                        <  1:732314‑M2/106‑37 (MQ=255)
                                                tCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttat                                                                                                        >  2:732314‑M2/1‑70 (MQ=255)
                                                       cTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgca                                                                >  1:477589‑M2/1‑63 (MQ=255)
                                                        tGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcac                                                               <  2:771546‑M2/139‑78 (MQ=255)
                                                        tGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcac                                                               >  2:407476‑M2/1‑62 (MQ=255)
                                                                         aTTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgc                                              <  2:307020‑M2/139‑95 (MQ=255)
                                                                         aTTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggc                                                           <  1:647968‑M2/126‑82 (MQ=255)
                                                                         aTTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggc                                                           >  2:647968‑M2/1‑45 (MQ=255)
                                                                          ttCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgcc                                             <  2:806821‑M2/139‑96 (MQ=255)
                                                                          ttCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgcc                                             >  1:1018593‑M2/1‑44 (MQ=255)
                                                                              tGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtgg                                         >  1:355986‑M2/1‑40 (MQ=255)
                                                                               gCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatc                                                       >  1:169247‑M2/1‑39 (MQ=255)
                                                                               gCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatc                                                       <  2:169247‑M2/124‑86 (MQ=255)
                                                                                  gAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccgg                                     >  1:105916‑M2/1‑36 (MQ=255)
                                                                                     ttCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggtggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaag                                  <  1:617548‑M2/139‑107 (MQ=255)
                                                                                     ttCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggc                                                                                                                  <  2:996303‑M2/59‑27 (MQ=255)
                                                                                     ttCAAACCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggc                                                                                                                  >  1:996303‑M2/1‑33 (MQ=255)
                                                                                         aaCCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggc                              >  1:1080854‑M2/1‑29 (MQ=255)
                                                                                          aCCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggct                             >  2:873190‑M2/1‑28 (MQ=255)
                                                                                          aCCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagagg                               <  2:1057688‑M2/137‑110 (MQ=255)
                                                                                          aCCAGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagagg                               >  1:1057688‑M2/1‑28 (MQ=255)
                                                                                             aGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggctaac                          >  2:702076‑M2/1‑25 (MQ=255)
                                                                                             aGACGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggctaac                          >  1:383360‑M2/1‑25 (MQ=255)
                                                                                               aCGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggctaaccg                        <  2:806763‑M2/139‑117 (MQ=255)
                                                                                               aCGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggc                                                           >  2:155739‑M2/1‑23 (MQ=255)
                                                                                               aCGTCGTGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggc                                                           <  1:155739‑M2/104‑82 (MQ=255)
                                                                                                     tGCTGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggctaaccgtacatt                  <  1:1043344‑M2/139‑123 (MQ=255)
                                                                                                        tGGTTCACGGCGATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggctaaccgtacattgac               >  1:307524‑M2/1‑14 (MQ=255)
                                                                                                                   gATacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggctaaccgtacattgaccgggcatctgg    >  1:461810‑M2/1‑3 (MQ=255)
                                                                                                                    aTacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggctaaccgtacattgaccgggcatctggc   <  1:548875‑M2/139‑138 (MQ=255)
                                                                                                                    aTacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggctaaccgtacattgaccgggcatctggc   >  2:217094‑M2/1‑2 (MQ=255)
                                                                                                                    aTacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtgg                                         >  2:770174‑M2/1‑2 (MQ=255)
                                                                                                                    aTacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtgg                                         <  1:770174‑M2/101‑100 (MQ=255)
                                                                                                                     tacgacgacgctggcaaccagcctggcggcgttttatcagcgtattcctgttggtcacgttgaggctggtctgcgcacgggcgatctctattcgccgtggccggaagaggctaaccgtacattgaccgggcatctggcg  >  1:372629‑M2/1‑1 (MQ=255)
                                                                                                                     |                                                                                                                                          
CGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTACATTGACCGGGCATCTG  >  NC_000913/3970300‑3970555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.