Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,205	T→C	T116A (ACC→GCC)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,205	0	T	C	100.0%	191.8 / NA	52	T116A (ACC→GCC)	araD	L‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/24);  total (28/24)

CTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGG  >  NC_000913/66083‑66330                                                                                                                           |                                                                                                                              cTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGa                                                                                                                  >  1:143399/1‑136 (MQ=255) cTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGa                                                                                                                  <  2:143399/136‑1 (MQ=255) cTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATc                                                                                                               >  2:57320/1‑139 (MQ=255)  tGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCg                                                                                                              >  2:746198/1‑139 (MQ=255)   gTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGa                                                                                                             >  2:6914/1‑139 (MQ=255)   gTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTACCACTCTTATTCGCCGTTGATTTATGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTTCCGTAGTAATAGTCGGCCTGGGTGGCGCTGGTTGGTGGAAttgt                                                                                                             >  1:595620/1‑136 (MQ=255)             ggTTTCTTCGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCGGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCTGGTTGCTGGAATCGACTGACCCGcc                                                                                                   <  1:809419/139‑1 (MQ=255)             ggTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGcc                                                                                                   <  1:746198/139‑1 (MQ=255)                 tCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTgcg                                                                                               >  1:429488/1‑139 (MQ=255)                 tCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTgcg                                                                                               >  1:561525/1‑139 (MQ=255)                        tGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGAt                                                                                        >  2:957816/1‑139 (MQ=255)                        tGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGAt                                                                                        >  2:1033996/1‑139 (MQ=255)                        tGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGCCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCGGAt                                                                                        >  1:846646/1‑139 (MQ=255)                               aCCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGgtggcg                                                                                 >  2:75758/1‑139 (MQ=255)                               aCCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGgtggcg                                                                                 >  2:1086080/1‑139 (MQ=255)                                    tttCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGtggcgtggcg                                                                            <  2:495639/139‑1 (MQ=255)                                        ccACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAg                                                                        <  1:902917/139‑1 (MQ=255)                                        ccACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAg                                                                        <  1:1033996/139‑1 (MQ=255)                                        ccACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAg                                                                        <  2:561525/139‑1 (MQ=255)                                        ccACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAg                                                                        <  2:993762/139‑1 (MQ=255)                                        ccACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAg                                                                        <  1:1032879/139‑1 (MQ=255)                                         cACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGt                                                                       >  1:1065/1‑139 (MQ=255)                                             cATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGt                                                                   <  2:280672/139‑1 (MQ=255)                                              atatTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTa                                                                  >  2:979362/1‑139 (MQ=255)                                                   cGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGccc                                                                                            <  2:435418/108‑1 (MQ=255)                                                   cGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGccc                                                                                            >  1:435418/1‑108 (MQ=255)                                                      cGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGcc                                                                                             >  1:297485/1‑104 (MQ=255)                                                      cGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGcc                                                                                             <  2:297485/104‑1 (MQ=255)                                                      cGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAg                                                                        >  2:1036782/1‑125 (MQ=255)                                                      cGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAg                                                                        <  1:1036782/125‑1 (MQ=255)                                                      cGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTa                                                                  <  1:557078/131‑1 (MQ=255)                                                      cGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTa                                                                  >  2:557078/1‑131 (MQ=255)                                                      cGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAAt                                                          >  2:788321/1‑139 (MQ=255)                                                      cGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAAt                                                          >  2:786880/1‑139 (MQ=255)                                                      cGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAAt                                                          >  2:688650/1‑139 (MQ=255)                                                      cGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAAt                                                          <  1:979362/139‑1 (MQ=255)                                                      cGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAAt                                                          >  2:212934/1‑139 (MQ=255)                                                                     cGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAggg                                           <  1:786880/139‑1 (MQ=255)                                                                       gTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGcc                                                                                                   >  1:58812/1‑81 (MQ=255)                                                                       gTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGcc                                                                                                   <  2:58812/81‑1 (MQ=255)                                                                       gTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGAGCGTATGCACAATGCCGCCAATGGAGGGGa                                         >  1:968280/1‑139 (MQ=255)                                                                                  ggTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCa                                                                                           >  2:653580/1‑78 (MQ=255)                                                                                  ggTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATAGACTGACCCGCCTGCGCCCa                                                                                           <  1:653580/78‑1 (MQ=255)                                                                                    tGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATagag                            <  1:57320/139‑1 (MQ=255)                                                                                                   cGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGt                    <  2:537286/132‑1 (MQ=255)                                                                                                   cGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGt                    >  1:537286/1‑132 (MQ=255)                                                                                                   cGTAGAAATAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTgg             <  2:1065/139‑1 (MQ=255)                                                                                                        aaaTAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCgtgt        <  2:426371/139‑1 (MQ=255)                                                                                                          aTAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATg                                      <  2:1092182/107‑1 (MQ=255)                                                                                                          aTAGTCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATg                                      >  1:1092182/1‑107 (MQ=255)                                                                                                              tCGGCGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCggagg  >  1:181949/1‑139 (MQ=255)                                                                                                                  cGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTgcgc                                                                                              <  2:480672/43‑1 (MQ=255)                                                                                                                  cGTGGGTGGCGCCGGTTGCTGGAATCGACTGACCCGCCTgcgc                                                                                              >  1:480672/1‑43 (MQ=255)                                                                                                                      ggtggcGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCGGCCAATGGAGGGGAATGCCTGATGGAGCAGCCg                      >  1:708573/1‑111 (MQ=255)                                                                                                                      ggtggcGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCg                      <  2:708573/111‑1 (MQ=255)                                                                                                                           |                                                                                                                              CTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGG  >  NC_000913/66083‑66330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑