Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,316,440 C→T P261P (CCG→CCA)  trpA ← tryptophan synthase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,316,4400CT100.0% 157.2 / NA 45P261P (CCG→CCA) trpAtryptophan synthase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/28);  total (17/28)

TCGACCGGGGTAAGCGAAACGGTAAAAAGATAAATATTAAATGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATCGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCTCGATGATTTTAACAATGGCCGAACCAGAAATCGCGCCCGCAGCTCCTGCATCAATCGCT  >  NC_000913/1316305‑1316557
                                                                                                                                       |                                                                                                                     
tCGACCGGGGTAAGCGAAACGGTAAAAAGATAAATATTAAATGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGt                                                                                                                    <  1:517468/139‑1 (MQ=255)
   aCCGGGGTAAGCGAAACGGTAAAAAGATAAATATTAAATGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGt                                                                                                                 >  1:381548/1‑139 (MQ=255)
               gAAACGGTAAAAAGATAAATATTAAATGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTc                                                                                                     <  2:247483/139‑1 (MQ=255)
                  aCGGTAAAAAGATAAATATTAAATGAATTTAGGATTTTTCCGGCTTCTTTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTAACAGt                                                                                                  >  1:481247/1‑139 (MQ=255)
                      tAAAAAGATAAATATTAAATGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTgccg                                                                                              >  2:642759/1‑139 (MQ=255)
                        aaaaGATAAATATTAAATGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTgccgcc                                                                                            <  2:47142/139‑1 (MQ=255)
                            gATAAATATTAAATGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCa                                                                                        <  1:513849/139‑1 (MQ=255)
                               aaaTATTAAATGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTgccgc                                                                                             <  2:931302/131‑1 (MQ=255)
                               aaaTATTAAATGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTgccgc                                                                                             >  1:931302/1‑131 (MQ=255)
                                aaTATTAAATGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCAtttt                                                                                    >  1:952456/1‑139 (MQ=255)
                                    ttAAATGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTctct                                                                                <  2:887662/139‑1 (MQ=255)
                                     tAAATGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTg                                                                               <  2:881425/139‑1 (MQ=255)
                                      aaaTGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTgg                                                                              <  2:381548/139‑1 (MQ=255)
                                      aaaTGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTgg                                                                              <  1:961376/139‑1 (MQ=255)
                                       aaTGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTgg                                                                              >  2:1002155/1‑138 (MQ=255)
                                       aaTGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTgg                                                                              <  1:1002155/138‑1 (MQ=255)
                                       aaTGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGc                                                                             <  2:665333/139‑1 (MQ=255)
                                       aaTGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGc                                                                             <  1:1067181/139‑1 (MQ=255)
                                       aaTGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTT‑AAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCt                                                                            <  1:375152/139‑1 (MQ=255)
                                        aTGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCCTTGGTTGTACAAAAATTTTCAGTGCCGCCAGCATTTTCTCTGGCt                                                                            <  1:642759/139‑1 (MQ=255)
                                        aTGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCt                                                                            <  2:786307/139‑1 (MQ=255)
                                        aTGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCt                                                                            <  1:777907/139‑1 (MQ=255)
                                                  gATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATg                                                                  <  2:952456/139‑1 (MQ=255)
                                                    tttttCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGtt                                                                <  2:161297/139‑1 (MQ=255)
                                                       ttCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGc                                                                             >  2:216763/1‑123 (MQ=255)
                                                       ttCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGc                                                                             <  1:216763/123‑1 (MQ=255)
                                                       ttCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCt                                                             >  2:616232/1‑139 (MQ=255)
                                                          cGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAACCTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCTCga                                                          <  1:570453/139‑1 (MQ=255)
                                                                          agTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGACAGCATTTTCTCTGGCTCATTAATATGTTGCTCGATGATTTTAACAATGGc                                          >  2:29333/1‑139 (MQ=255)
                                                                           gTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCTCGATGATTTTAACAATGGcc                                         >  2:951507/1‑139 (MQ=255)
                                                                           gTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCTCGATGATTTTAACAATGGcc                                         <  1:528960/139‑1 (MQ=255)
                                                                                     cgccAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCCCTGGCTCATTAATATGTTGCTCGATGATTTTAACAATGGCCGAACCAGaaa                               >  2:1023952/1‑139 (MQ=255)
                                                                                      gccAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCTCGATGAttt                                                    <  2:453144/117‑1 (MQ=255)
                                                                                      gccAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCTCGATGAttt                                                    >  1:453144/1‑117 (MQ=255)
                                                                                      gccAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCTCGATGATTTTAACAATGGCCg                                        >  2:3289/1‑129 (MQ=255)
                                                                                      gccAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCTCGATGATTTTAACAATGGCCg                                        <  1:3289/129‑1 (MQ=255)
                                                                                              aaCTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCTCGATGATTTTAACAATGGCCGAACCAGAAATCGCGCCCg                      <  2:350307/139‑1 (MQ=255)
                                                                                                                 aaCTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCTCGATGATTTTAACAATGGCCGAACCAGAAATCGCGCCCGCAGCTCCTGCATCATTCgc   >  2:379107/1‑139 (MQ=255)
                                                                                                                  aCTGCGCGTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCTCGATGATTTTAACAATGGCCGAACCAGAAATCGCGCCCGCAGCTCCTGCATCAATCgct  <  1:556587/139‑1 (MQ=255)
                                                                                                                      cgcgTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCt                                                                            >  2:787080/1‑61 (MQ=255)
                                                                                                                      cgcgTCGCCGCTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCt                                                                            <  1:787080/61‑1 (MQ=255)
                                                                                                                           cgccgcTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTgg                                                                              >  2:325063/1‑54 (MQ=255)
                                                                                                                           cgccgcTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTgg                                                                              <  1:325063/54‑1 (MQ=255)
                                                                                                                           cgccgcTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCTCGATGATTTTAACAATgg                                           >  2:1006931/1‑89 (MQ=255)
                                                                                                                           cgccgcTTTCATTGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCTCGATGATTTTAACAATgg                                           <  1:1006931/89‑1 (MQ=255)
                                                                                                                                       |                                                                                                                     
TCGACCGGGGTAAGCGAAACGGTAAAAAGATAAATATTAAATGAATTTAGGATTTTTCCGGCTTCATTAAAGAAAGTTAAAATGCCGCCAGCGGAACTGGCGGCTGTGGGATTAACTGCGCGTCGCCGCTTTCATCGGTTGTACAAAAACTTTCAGTGCCGCCAGCATTTTCTCTGGCTCATTAATATGTTGCTCGATGATTTTAACAATGGCCGAACCAGAAATCGCGCCCGCAGCTCCTGCATCAATCGCT  >  NC_000913/1316305‑1316557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑