Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,364,513	T→C	R94R (CGT→CGC)	puuB →	gamma‑glutamylputrescine oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,364,513	0	T	C	100.0%	108.2 / NA	30	R94R (CGT→CGC)	puuB	gamma‑glutamylputrescine oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/17);  total (13/17)

GCTTTGACGTAGTGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAA  >  NC_000913/1364380‑1364645                                                                                                                                      |                                                                                                                                     gCTTTGACGTAGTGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCt                                                                                                                                 <  2:730769/139‑1 (MQ=255)         gTAGTGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGgcagca                                                                                                                         <  1:391310/139‑1 (MQ=255)                   tcGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGa                                                                                                               <  2:1050832/139‑1 (MQ=255)                        gCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAggtg                                                                                                          <  2:792947/139‑1 (MQ=255)                            cACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTga                                                                                                      >  2:659206/1‑139 (MQ=255)                                   cGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGatcatc                                                                                               >  2:928720/1‑139 (MQ=255)                                        ttGGCGCAAGCGGGCGCAACGGCGGACAGCTTGTGAACTCCTACCGCCGCCACATCGACGTGATCGAAAAAAGCGACGGCATGGACACCGCCCGCATGCTCGGCAGCACGATGTTCGAAGGTGGTGAGATCATCcgcgt                                                                                          <  1:631857/139‑2 (MQ=255)                                         tGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGaa                                                                                         <  1:928720/139‑1 (MQ=255)                                              cAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTAt                                                                                    <  2:927023/139‑1 (MQ=255)                                                         aaTGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATc                                                                         >  2:586765/1‑139 (MQ=255)                                                            ggCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGa                                                                      <  2:3447/139‑1 (MQ=255)                                                                     cTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCCGCATGATCTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCg                                                             >  2:86408/1‑139 (MQ=255)                                                                       tgtgAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGAc                                                           >  2:133223/1‑139 (MQ=255)                                                                                cTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGcccc                                                  >  1:607302/1‑139 (MQ=255)                                                                                        gcgACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCt                                          >  2:883454/1‑139 (MQ=255)                                                                                        gcgACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCt                                          >  2:71020/1‑139 (MQ=255)                                                                                         cgACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCaaa                                                                                >  1:158287/1‑100 (MQ=255)                                                                                         cgACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCaaa                                                                                <  2:158287/100‑1 (MQ=255)                                                                                               cGACGTAATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCGCGGCGGCCTGTTtgtg                                   <  1:92794/139‑1 (MQ=255)                                                                                                  cGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCg                                <  2:1060910/139‑1 (MQ=255)                                                                                                    tGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAg                                                                                                             >  2:63278/1‑60 (MQ=255)                                                                                                    tGATCGAAAAAAGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAg                                                                                                             <  1:63278/60‑1 (MQ=255)                                                                                                              aaGCTACGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATaaa                    <  1:679059/139‑1 (MQ=255)                                                                                                                    cGGCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTc              <  1:957232/139‑1 (MQ=255)                                                                                                                     ggCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCg             >  1:660398/1‑139 (MQ=255)                                                                                                                     ggCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGATATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCg             >  1:392022/1‑139 (MQ=255)                                                                                                                      gCATGGACACCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGc            <  1:133223/139‑1 (MQ=255)                                                                                                                              acCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCca          <  1:896427/133‑1 (MQ=255)                                                                                                                              acCGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCca          >  2:896427/1‑133 (MQ=255)                                                                                                                                cGCCCGCATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGaa  <  1:659206/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     GCTTTGACGTAGTGGTTCTCGAAGCCTCACGCATCGGCTTTGGCGCAAGCGGGCGCAATGGCGGACAGCTTGTGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAA  >  NC_000913/1364380‑1364645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑