Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,632,151 | T→C | pseudogene (135/1284 nt) | ydfJ ← | pseudogene, MFS transporter family, interrupted by Qin prophage,Phage or Prophage Related, putative transport protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,632,151 | 0 | T | C | 98.0% | 186.8 / NA | 51 | pseudogene (135/1284 nt) | ydfJ | pseudogene, MFS transporter family, interrupted by Qin prophage,Phage or Prophage Related, putative transport protein |
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (28/22); minor base A (1/0); total (29/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGTGCAAAAACACCAATCTGTGCATAGGTCGGTAACACACCAATTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCTCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAGGAAAAAATATTTCATGAAACACTAACGCTGCGCCGAGCGAATATAACTGGAAATCCATA > NC_000913/1632017‑1632286 | ggTGCAAAAACACCAATCTGTGCATAGGTCGGTAACACACCAATTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGa < 1:1050001/139‑1 (MQ=255) gCAAAAACACCAATCTGTGCATAGGTCGGTAACACACCAATTAATGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACaa > 1:784340/1‑139 (MQ=255) gCAAAAACACCAATCTGTGCATAGGTCGGTAACACACCAATTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACaa > 1:679725/1‑139 (MQ=255) acCAATCTGTGCATAGGTCGGTAACACACCAATTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGcc > 2:732949/1‑139 (MQ=255) cAATCTGTGCATAGGTCGGTAACACACCAATTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCcac < 2:679725/139‑1 (MQ=255) aTCTGTGCATAGGTCGGTAACACACCAATTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACAt < 1:853883/139‑1 (MQ=255) gCATAGGTCGGTAACACACCAATTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATAcg < 2:407711/125‑1 (MQ=255) gCATAGGTCGGTAACACACCAATTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATAcg > 1:407711/1‑125 (MQ=255) tAGGTCGGTAACACACCAATTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCAc < 1:528866/139‑1 (MQ=255) gTCGGTAACACACCAATTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGt > 1:131798/1‑139 (MQ=255) ggTAACACACCAATTAATGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTATGCCCTCTTCTGTCGCCCATTTTGCCTATAATAAATGTCACGACAATAGCCGCCACATAGCCTGCACCGTAgg > 1:425982/1‑139 (MQ=255) ggTAACACACCAATTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCACCGACAATACGCGCCACATAACCTGCACCGTAgg > 1:786819/1‑139 (MQ=255) cacCAATTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCAt > 1:970484/1‑139 (MQ=255) acCAATTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCAtt > 1:293410/1‑139 (MQ=255) aTTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCa < 2:703767/139‑1 (MQ=255) aTTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCa > 1:805517/1‑139 (MQ=255) aCAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGcc > 2:174753/1‑139 (MQ=255) aTCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTg < 2:1063662/139‑1 (MQ=255) aTCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTg > 2:518257/1‑139 (MQ=255) aTCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTg < 2:1057689/139‑1 (MQ=255) tCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCt > 1:975858/1‑101 (MQ=255) tCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCt < 2:975858/101‑1 (MQ=255) tcatGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTtga > 1:276445/1‑139 (MQ=255) tGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTtgatga > 1:238723/1‑139 (MQ=255) ggTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGc > 2:355437/1‑111 (MQ=255) ggTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGc < 1:355437/111‑1 (MQ=255) aTGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAg > 1:969334/1‑139 (MQ=255) aataaAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAGGaaaa < 2:293410/139‑1 (MQ=255) agCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCG‑AAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTcc < 2:463519/88‑1 (MQ=255) agCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCG‑AAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTcc > 1:463519/1‑88 (MQ=255) cACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATAcgc < 1:255936/61‑1 (MQ=255) cACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATAcgc > 2:255936/1‑61 (MQ=255) cACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGAtt < 2:460553/122‑1 (MQ=255) cACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGAtt > 1:460553/1‑122 (MQ=255) cACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAGGAAAAAATATTTCa > 1:985373/1‑139 (MQ=255) cACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAGGAAAAAATATTTCa > 2:651203/1‑139 (MQ=255) ttttttACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAGGAAAAAATATTTCATGa < 2:985373/139‑1 (MQ=255) ttttttACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAGGAAAAAATATTTCATGa < 2:131798/139‑1 (MQ=255) tttACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAGGAAAAAATATTTCATGAAac > 1:951440/1‑139 (MQ=255) tACGCCCTATTCTGTCGCCCATTTTGCCGAAGATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCa > 2:422871/1‑99 (MQ=255) tACGCCCTATTCTGTCGCCCATTTTGCCGAAGATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCa < 1:422871/99‑1 (MQ=255) ccTATTCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAGGAAAAAATATTTCATGAAACACTAACg > 2:453041/1‑139 (MQ=255) ttCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACgcgc < 2:691954/46‑1 (MQ=255) ttCTGTCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACgcgc > 1:691954/1‑46 (MQ=255) tCGCCCATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAGGAAAAAATATTTCATGAAACACTAACGCTGCGCCGa > 1:933378/1‑139 (MQ=255) cATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGAtt < 2:481770/95‑1 (MQ=255) cATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGAtt > 1:481770/1‑95 (MQ=255) cATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAGGAAAAAATATTTCATGa < 2:1059346/115‑1 (MQ=255) cATTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAGGAAAAAATATTTCATGa > 1:1059346/1‑115 (MQ=255) aTTTTGCCGAAAATAAATGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAGGAAAAAATATTTCATGAAACACTAACGCTGCGCCGAGCGAat < 2:933378/139‑1 (MQ=255) tGCCCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAGGAAAAAATATTTCATGAAACACTAACGCTGCGCCGAGCGAATATAACTGGAAATCCATa < 2:840695/139‑1 (MQ=255) | GGTGCAAAAACACCAATCTGTGCATAGGTCGGTAACACACCAATTAAGGTGGTACAGATCCCCATCATGGTGATGGTAATAAAGAGCACTTTTTTACGCCCTATTCTGTCGCCCATTTTGCCGAAAATAAATGCTCCGACAATACGCGCCACATAACCTGCACCGTAGGTTCCCATTGCCAGAATTAACGCCATTGCCGTTGATGATTCAGGAAAAAATATTTCATGAAACACTAACGCTGCGCCGAGCGAATATAACTGGAAATCCATA > NC_000913/1632017‑1632286 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |