Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,675,350	A→G	G337G (GGT→GGC)	pntB ←	pyridine nucleotide transhydrogenase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,675,350	0	A	G	100.0%	198.5 / NA	53	G337G (GGT→GGC)	pntB	pyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/25);  total (28/25)

GTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCG  >  NC_000913/1675216‑1675485                                                                                                                                       |                                                                                                                                        gtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCAc                                                                                                                                     >  1:274792/1‑139 (MQ=255) gtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCAc                                                                                                                                     >  2:790981/1‑139 (MQ=255)    tcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGCTACCGAAACGCACATTAATGCCACGAg                                                                                                                                  <  1:662987/139‑1 (MQ=255)    tcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAg                                                                                                                                  <  2:767912/139‑1 (MQ=255)      aGCAACGTCATCATTGATCTCGTCCATTTCCAGCACGATTTCATACGGTACTCTTGCTTCAGCCAGCAATACGTTCTTACGTCCAGGCAAACGCCCCTCGACCGGCTGGATATCGAAACGCACATTAATGCCACGAgcg                                                                                                                                <  1:157894/139‑1 (MQ=255)        cAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAgcgcg                                                                                                                              >  2:633931/1‑139 (MQ=255)              catcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAAttt                                                                                                                        >  1:864926/1‑139 (MQ=255)               atcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTtc                                                                                                                       <  1:790981/139‑1 (MQ=255)                tcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCGACGAGCGCGCAATTtct                                                                                                                      >  2:684438/1‑139 (MQ=255)                       tctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAgaatt                                                                                                               >  2:148913/1‑135 (MQ=255)                       tctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAAt                                                                                                               >  1:1108790/1‑139 (MQ=255)                       tctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAAt                                                                                                               >  1:966978/1‑139 (MQ=255)                           gTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCa                                                                                                           >  2:680467/1‑139 (MQ=255)                            tCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                                          <  1:977569/139‑1 (MQ=255)                              cATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCg                                                                                                        <  1:789420/139‑1 (MQ=255)                                 ttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACa                                                                                                     <  2:482049/139‑1 (MQ=255)                                          gATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCCGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAg                                                                                                                                  >  1:483046/1‑101 (MQ=255)                                          gATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCCGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAg                                                                                                                                  <  2:483046/101‑1 (MQ=255)                                           aTGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgcg                                                                                           >  1:410669/1‑139 (MQ=255)                                            tGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgcgc                                                                                          >  1:637376/1‑139 (MQ=255)                                                      tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCTGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                                >  1:455158/1‑139 (MQ=255)                                                      tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAAt                                                                                                                          <  2:1073914/97‑1 (MQ=255)                                                      tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAAt                                                                                                                          >  1:1073914/1‑97 (MQ=255)                                                      tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                                >  1:169084/1‑139 (MQ=255)                                                      tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                                >  2:1006965/1‑139 (MQ=255)                                                      tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                                >  2:767693/1‑139 (MQ=255)                                                            tGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCTGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATg                                                                          <  2:455158/139‑1 (MQ=255)                                                            tGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATg                                                                          <  2:864926/139‑1 (MQ=255)                                                                tCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGt                                                                      >  2:12950/1‑139 (MQ=255)                                                                    ccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAccc                                                                  <  2:76463/139‑1 (MQ=255)                                                                      agcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCg                                                                <  2:894297/139‑1 (MQ=255)                                                                        cAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGa                                                              <  2:94169/139‑1 (MQ=255)                                                                                tCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGAt                                                      <  2:996845/139‑1 (MQ=255)                                                                                           ggCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATggg                                           <  2:274792/139‑1 (MQ=255)                                                                                           ggCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATggg                                           <  1:858453/139‑1 (MQ=255)                                                                                            gCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGa                                          >  2:609560/1‑139 (MQ=255)                                                                                            gCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGa                                          >  2:618813/1‑139 (MQ=255)                                                                                                aCGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGttt                                      >  2:833926/1‑139 (MQ=255)                                                                                                 cGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGa                                                              <  2:492452/114‑1 (MQ=255)                                                                                                 cGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGa                                                              >  1:492452/1‑114 (MQ=255)                                                                                                 cGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGCTCACTGAATGGGAGtttt                                     >  1:118022/1‑139 (MQ=255)                                                                                                 cGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtttt                                     >  1:30726/1‑139 (MQ=255)                                                                                                        cgACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcagcag                              <  1:833926/139‑1 (MQ=255)                                                                                                                   aTACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGa                                                              >  1:531045/1‑96 (MQ=255)                                                                                                                   aTACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGa                                                              <  2:531045/96‑1 (MQ=255)                                                                                                                     aCCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTctct                 <  2:1106133/139‑1 (MQ=255)                                                                                                                         aaaCGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCg                                                                >  1:929892/1‑88 (MQ=255)                                                                                                                         aaaCGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCg                                                                >  1:825713/1‑88 (MQ=255)                                                                                                                         aaaCGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCg                                                                <  2:825713/88‑1 (MQ=255)                                                                                                                         aaaCGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCg                                                                <  2:929892/88‑1 (MQ=255)                                                                                                                         aaaCGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGt                                                            <  2:139204/92‑1 (MQ=255)                                                                                                                         aaaCGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGt                                                            >  1:139204/1‑92 (MQ=255)                                                                                                                                    aaTGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTcg  <  2:783109/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        GTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCG  >  NC_000913/1675216‑1675485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑