Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,708,192 T→C V425A (GTT→GCT)  rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,1920TC100.0% 100.8 / NA 27V425A (GTT→GCT) rsxCSoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/9);  total (18/9)

GCGCCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGG  >  NC_000913/1708060‑1708320
                                                                                                                                    |                                                                                                                                
gcgcCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGTAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAGGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGccc                                                                                                                            <  1:1084072/139‑1 (MQ=255)
gcgcCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGccc                                                                                                                            <  2:730641/139‑1 (MQ=255)
  gcCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGa                                                                                                                          <  1:263653/139‑1 (MQ=255)
   ccTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAg                                                                                                                         <  2:359728/139‑1 (MQ=255)
    cTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGc                                                                                                                        <  2:756650/139‑1 (MQ=255)
      gtgCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCaa                                                                                                                      >  1:118588/1‑139 (MQ=255)
                                  gCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAg                                                                                          >  2:935294/1‑139 (MQ=255)
                                           gTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCt                                                                                 <  1:935294/139‑1 (MQ=255)
                                                    gTTCAGCATAGGTCAGCAACACGATAAAGCTACCACGGATAACGTTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCGCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCg                                                                        >  2:741789/1‑139 (MQ=255)
                                                    gTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTcccc                                                                                                              <  1:214989/101‑1 (MQ=255)
                                                    gTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTcccc                                                                                                              >  2:214989/1‑101 (MQ=255)
                                                               gTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCa                                                             >  1:377143/1‑139 (MQ=255)
                                                                         cGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAg                                                   >  2:341811/1‑139 (MQ=255)
                                                                                    ccACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAata                                                                                                                    >  1:710921/1‑63 (MQ=255)
                                                                                    ccACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAata                                                                                                                    <  2:710921/63‑1 (MQ=255)
                                                                                    ccACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGa                                        >  2:73549/1‑139 (MQ=255)
                                                                                    ccACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGa                                        >  1:248000/1‑139 (MQ=255)
                                                                                         cATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCa                                   >  1:39584/1‑139 (MQ=255)
                                                                                           tAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCaaa                                 >  2:484192/1‑139 (MQ=255)
                                                                                                 tGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGt                           >  2:638280/1‑139 (MQ=255)
                                                                                                 tGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGt                           >  2:803650/1‑139 (MQ=255)
                                                                                                   cTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGttt                         >  2:1073289/1‑139 (MQ=255)
                                                                                                                 tgtgGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAgg           <  2:118588/139‑1 (MQ=255)
                                                                                                                         ttGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTg   >  2:576670/1‑139 (MQ=255)
                                                                                                                         ttGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTg   >  2:216874/1‑139 (MQ=255)
                                                                                                                         ttGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTg   >  1:420613/1‑139 (MQ=255)
                                                                                                                         ttGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTgg  >  2:923350/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                
GCGCCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGG  >  NC_000913/1708060‑1708320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: