Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,869,266	G→A	Q104Q (CAG→CAA)	yeaH →	UPF0229 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,869,266	0	G	A	100.0%	117.9 / NA	34	Q104Q (CAG→CAA)	yeaH	UPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/17);  total (17/17)

CGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCG  >  NC_000913/1869134‑1869393                                                                                                                                     |                                                                                                                                cGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCACCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAgccagc                                                                                                                           >  1:1009788/1‑139 (MQ=255)    aCCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGccagccag                                                                                                                        <  2:320262/139‑1 (MQ=255)     ccGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAgg                                                                                                                       >  1:497491/1‑139 (MQ=255)      cGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGa                                                                                                                      <  1:1074601/139‑1 (MQ=255)                     ggCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGa                                                                                                       <  2:345235/139‑1 (MQ=255)                                             tGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGaa                                                                               >  2:1103624/1‑139 (MQ=255)                                                            aCCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGa                                                                >  1:435249/1‑139 (MQ=255)                                                             ccATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGAt                                                               <  2:894132/139‑1 (MQ=255)                                                                tttCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGa                                                                         <  2:925404/126‑1 (MQ=255)                                                                tttCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGa                                                                         >  1:925404/1‑126 (MQ=255)                                                                tttCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTg                                                            <  2:854729/139‑1 (MQ=255)                                                                 ttCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGc                                                           >  2:1012654/1‑139 (MQ=255)                                                                              aCCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGAttt                                              >  2:106070/1‑139 (MQ=255)                                                                                 gAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGc                                           <  1:106070/139‑1 (MQ=255)                                                                                       aaCGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTAcc                                     >  2:1031979/1‑139 (MQ=255)                                                                                         cGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTg                                                                                                                 <  2:243961/61‑1 (MQ=255)                                                                                         cGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTg                                                                                                                 >  1:243961/1‑61 (MQ=255)                                                                                         cGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGa                                   >  2:919263/1‑139 (MQ=255)                                                                                             cccAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGaa                                                                               <  1:915368/91‑1 (MQ=255)                                                                                             cccAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGaa                                                                               >  2:915368/1‑91 (MQ=255)                                                                                                   gTGGCGGCGGAGGTTCCGGGAGTTGGCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACa                         <  2:744795/139‑1 (MQ=255)                                                                                                      gcggcgGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAg                                                                                       >  1:797792/1‑74 (MQ=255)                                                                                                      gcggcgGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAg                                                                                       <  2:797792/74‑1 (MQ=255)                                                                                                      gcggcgGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGc                                           >  1:730598/1‑118 (MQ=255)                                                                                                      gcggcgGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGc                                           <  2:730598/118‑1 (MQ=255)                                                                                                      gcggcgGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACaaaa                      >  1:390157/1‑139 (MQ=255)                                                                                                       cggcggAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAAc                     >  2:371029/1‑139 (MQ=255)                                                                                                                    ggCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGc        <  1:89441/139‑1 (MQ=255)                                                                                                                         tGGTCAGGTCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGAcc   <  1:1012654/139‑1 (MQ=255)                                                                                                                         tGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGAcc   <  1:894815/139‑1 (MQ=255)                                                                                                                         tGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGAcc   >  2:647233/1‑139 (MQ=255)                                                                                                                         tGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGAcc   <  1:371029/139‑1 (MQ=255)                                                                                                                         tGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGAcc   <  2:270713/139‑1 (MQ=255)                                                                                                                          gggcAGGGCCAAGCCAGCCAGGATGGTGAAGGACAGGATGACTTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAAAAACGCCAGCTGACCg  >  1:1046318/4‑139 (MQ=255)                                                                                                                                ggccaagccaGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATc                                                                    >  1:244454/1‑67 (MQ=255)                                                                                                                                ggccaagccaGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATc                                                                    <  2:244454/67‑1 (MQ=255)                                                                                                                                     |                                                                                                                                CGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCG  >  NC_000913/1869134‑1869393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑