Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,904,586 T→C V231A (GTC→GCC)  manZ → mannose‑specific enzyme IID component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,904,5860TC100.0% 180.9 / NA 48V231A (GTC→GCC) manZmannose‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/26);  total (22/26)

AAAACTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGT  >  NC_000913/1904452‑1904719
                                                                                                                                      |                                                                                                                                     
aaaaCTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAg                                                                                                                                   <  1:437209/139‑1 (MQ=255)
 aaaCTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGa                                                                                                                                  <  1:130301/139‑1 (MQ=255)
 aaaCTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGa                                                                                                                                  <  2:40503/139‑1 (MQ=255)
      gACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTAtt                                                                                                                             >  1:952264/1‑139 (MQ=255)
            aGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGAc                                                                                                                       <  2:223221/139‑1 (MQ=255)
                       tCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGcc                                                                                                            <  1:515968/139‑1 (MQ=255)
                        ccTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCa                                                                                                           <  2:40282/139‑1 (MQ=255)
                                            gggCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCActgctgct                                                                                       >  2:324423/1‑139 (MQ=255)
                                            gggCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGATAATGCCAGGCCTGGTACCActgctgct                                                                                       >  2:666774/1‑139 (MQ=255)
                                             ggCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTa                                                                                                                               >  2:7218/1‑98 (MQ=255)
                                             ggCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTa                                                                                                                               <  1:7218/98‑1 (MQ=255)
                                                 ttGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCt                                                                                  <  2:267098/139‑1 (MQ=255)
                                                      tAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCt                                                                             <  2:709437/139‑1 (MQ=255)
                                                       aaCAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCtt                                                                            >  1:787468/1‑139 (MQ=255)
                                                        aCAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTg                                                                           <  2:223870/139‑1 (MQ=255)
                                                            gTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTAtg                                                                       >  2:561502/1‑139 (MQ=255)
                                                            gTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTAtg                                                                       >  2:961193/1‑139 (MQ=255)
                                                              ggACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTAtgtg                                                                     >  2:989447/1‑139 (MQ=255)
                                                              ggACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTAtgtg                                                                     >  1:175449/1‑139 (MQ=255)
                                                              ggACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTAtgtg                                                                     >  1:1105705/1‑139 (MQ=255)
                                                               gACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTgg                                                                    >  2:426040/1‑139 (MQ=255)
                                                                     tGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTg                                                              <  2:787468/139‑1 (MQ=255)
                                                                      gTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTgc                                                             >  2:1013116/1‑139 (MQ=255)
                                                                         aaCATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCa                                                          >  1:588435/1‑139 (MQ=255)
                                                                              cccGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCaaaaaa                                                     >  2:585430/1‑139 (MQ=255)
                                                                              cccGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCaaaaaa                                                     >  2:93369/1‑139 (MQ=255)
                                                                                cGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGt                                                   >  1:426789/1‑139 (MQ=255)
                                                                                cGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGt                                                   >  2:369106/1‑139 (MQ=255)
                                                                                   tGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTaa                                                <  1:860294/139‑1 (MQ=255)
                                                                                   tGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTaa                                                <  1:1013116/139‑1 (MQ=255)
                                                                                    ggTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                               <  1:585430/139‑1 (MQ=255)
                                                                                    ggTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                               <  2:740362/139‑1 (MQ=255)
                                                                                    ggTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                               <  2:1012011/139‑1 (MQ=255)
                                                                                          ctctcGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCtg                                         <  1:942665/139‑1 (MQ=255)
                                                                                              cGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCACAAAAAAGTTAACCCGCTGTGGa                                     <  2:588435/139‑1 (MQ=255)
                                                                                               gCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGat                                    <  2:427180/139‑1 (MQ=255)
                                                                                               gCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGat                                    <  1:1000041/139‑1 (MQ=255)
                                                                                                       gACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTg                            >  1:235979/1‑139 (MQ=255)
                                                                                                         ccAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCtgtg                                       >  2:470178/1‑126 (MQ=255)
                                                                                                         ccAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCtgtg                                       <  1:470178/126‑1 (MQ=255)
                                                                                                          cAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                               >  1:906282/1‑117 (MQ=255)
                                                                                                          cAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                               <  2:906282/117‑1 (MQ=255)
                                                                                                           aGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGctt                        >  1:517131/1‑139 (MQ=255)
                                                                                                                    aaGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCAt               <  2:879572/139‑1 (MQ=255)
                                                                                                                          acGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTAt         <  2:41700/139‑1 (MQ=255)
                                                                                                                           cGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATc        <  1:635376/139‑1 (MQ=255)
                                                                                                                                 tactGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGt  <  1:93369/139‑1 (MQ=255)
                                                                                                                                 tactGCCCAGACTATTCGGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGATGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGt  >  1:686912/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                     
AAAACTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGT  >  NC_000913/1904452‑1904719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑