| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,240,094 | T→C | A85A (GCA→GCG) | mglB ← | methyl‑galactoside transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,240,094 | 0 | T | C | 97.5% | 148.0 / NA | 40 | A85A (GCA→GCG) | mglB | methyl‑galactoside transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (20/19); minor base A (1/0); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGCTTTGTCGTAGCTATCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGTGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCACTACA > NC_000913/2239964‑2240226 | agGCTTTGTCGTAGCTATCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCAc > 1:93479/1‑139 (MQ=255) tttGTCGTAGCTATCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCt > 2:157189/1‑139 (MQ=255) tttGTCGTAGCTATCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCt > 2:906726/1‑139 (MQ=255) tGTCGTAGCTATCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCttt > 2:436677/1‑139 (MQ=255) cTATCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTCTCGCCAGCa < 2:744763/139‑1 (MQ=255) ccAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATAc > 1:215599/1‑139 (MQ=255) cAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACg < 1:139403/139‑1 (MQ=255) cAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACg < 2:907294/139‑1 (MQ=255) gcCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGa > 1:610950/1‑139 (MQ=255) ccTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGAt > 2:360669/1‑139 (MQ=255) gaCGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGt < 1:698028/139‑1 (MQ=255) ggTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCa > 2:649230/1‑118 (MQ=255) ggTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCa < 1:649230/118‑1 (MQ=255) ttCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGc > 2:641567/1‑139 (MQ=255) cTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGa > 1:594887/1‑123 (MQ=255) cTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGa < 2:594887/123‑1 (MQ=255) tgttgAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGa > 1:330834/1‑139 (MQ=255) accaccGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCt < 1:360669/139‑1 (MQ=255) accGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTgag < 1:157189/139‑1 (MQ=255) ggCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAAt < 2:946583/139‑1 (MQ=255) ttttGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAAtcattca > 1:600660/1‑139 (MQ=255) ttGCCCACACGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATtcatc > 1:816018/1‑139 (MQ=255) aggcgaTTTCTCAATCCCGGTACCCGCAGCTGCCGCGTCAACCAGGCTGATTGCCTGCGCCTTCGCCCCTTTCCCCAGCAATACGTCTATCTGTTCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCagcagc < 1:60399/137‑1 (MQ=255) aTCACCGTACCCGCAGCTGCCGGGTCAACCAGTTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGgcg < 2:1044865/139‑1 (MQ=255) tCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGgcgc < 1:135893/139‑1 (MQ=255) cGTCCCCGGAGCGGCCGGGTCAAGCAGGTTCATCGCCAGAGTCTCCACCCCTTTCCCCAGCCATACGTCGGTCTGATAGTTCTGCTTGGACTTGTCATCCTGAGAATCATTCATCAGCAGCAGAACATCTGGCGCggta > 1:871972/1‑137 (MQ=255) cGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGAc < 2:312515/91‑1 (MQ=255) cGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGAc > 1:312515/1‑91 (MQ=255) cGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCt > 2:186574/1‑139 (MQ=255) cGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCt > 2:514433/1‑139 (MQ=255) cAGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATc > 1:312478/1‑139 (MQ=255) aaGCTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATc < 2:842856/138‑1 (MQ=255) cTGCCGGGTCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTg < 1:436677/139‑1 (MQ=255) tCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAg > 2:200097/1‑41 (MQ=255) tCAACCAGGTTGATTGCCAGCGCCTTCACCCCTTTCGCCAg < 1:200097/41‑1 (MQ=255) ggTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTgcg < 1:514433/139‑1 (MQ=255) ggTTGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTgcg < 1:201943/139‑1 (MQ=255) ttGATTGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCa > 1:1017858/1‑139 (MQ=255) ttGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCACTAc < 1:641567/139‑1 (MQ=255) tGCCAGCGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCACTACa > 2:312990/1‑139 (MQ=255) | AGGCTTTGTCGTAGCTATCCAGCGCCTTACGAGACGGTTCTTTGTTGAAGAAAACCACCGGCACGTTTTGCCCACGCGCTTTCTCAATCACCGTACCCGCAGCTGCCGGGTCAACCAGGTTGATTGCCAGTGCCTTCACCCCTTTCGCCAGCAATACGTCGATCTGATCGTTCTGCTTGGACTGGTCATTCTGAGAATCATTCATCAGCAGCTGAACATCTGGCGCGGCTTTCGCATCTTGCTCAATAGCCTTGCGCACTACA > NC_000913/2239964‑2240226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |