Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,414,597	T→C	G376G (GGT→GGC)	ackA →	acetate kinase A and propionate kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,414,597	0	T	C	100.0%	154.6 / NA	41	G376G (GGT→GGC)	ackA	acetate kinase A and propionate kinase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/20);  total (21/20)

TGGTGGTATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCG  >  NC_000913/2414462‑2414726                                                                                                                                        |                                                                                                                                  tggtggTATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCAcc                                                                                                                                >  2:629551/1‑139 (MQ=255) tggtggTATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCAcc                                                                                                                                >  1:811799/1‑139 (MQ=255)   gtggtATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCg                                                                                                                              <  2:888305/139‑1 (MQ=255)    tggtATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGt                                                                                                                             >  2:866383/1‑139 (MQ=255)      gtATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGt                                                                                                                             >  1:832893/1‑137 (MQ=255)      gtATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGt                                                                                                                             <  2:832893/137‑1 (MQ=255)         tCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGc                                                                                                                        >  1:165384/1‑139 (MQ=255)                       gCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCccaa                                                                                                          >  1:32000/1‑139 (MQ=255)                        cAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCccaac                                                                                                         <  1:1078235/139‑1 (MQ=255)                        cAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCccaac                                                                                                         <  1:629551/139‑1 (MQ=255)                                   gAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAAc                                                                                              <  1:528658/139‑1 (MQ=255)                                                    aCTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCccaac                                                                                                         >  1:304171/1‑111 (MQ=255)                                                    aCTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCccaac                                                                                                         <  2:304171/111‑1 (MQ=255)                                                    aCTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGAc                                                                             >  1:186364/1‑139 (MQ=255)                                                         gCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAg                                                                        <  1:304042/139‑1 (MQ=255)                                                         gCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAg                                                                        >  1:251699/1‑139 (MQ=255)                                                         gCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAg                                                                        >  1:89349/1‑139 (MQ=255)                                                          cGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAgc                                                                       >  1:837680/1‑139 (MQ=255)                                                              cTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAgccgcc                                                                   >  1:80866/1‑139 (MQ=255)                                                                      tGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGcc                                                           >  1:603392/1‑139 (MQ=255)                                                                      tGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGACAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGcc                                                           <  2:427269/139‑1 (MQ=255)                                                                             gATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTc                                                    >  2:68155/1‑139 (MQ=255)                                                                             gATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTc                                                    >  1:53777/1‑139 (MQ=255)                                                                              aTCATGAACGCAACCTGGCTGCACTTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTca                                                   <  1:209805/139‑1 (MQ=255)                                                                                  tGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCAc                                                                                                                                 >  2:1094034/1‑57 (MQ=255)                                                                                  tGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACAcc                                               <  2:165384/139‑1 (MQ=255)                                                                                                cTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGTAAGACGCGAGCCGCCTGACTGACTGATTTCACACCGCCAGCTCAGCTgg                                 <  2:489513/139‑1 (MQ=255)                                                                                                cTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTgg                                 <  2:186364/139‑1 (MQ=255)                                                                                                     cGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTc                                                                                                                            <  1:1089678/43‑1 (MQ=255)                                                                                                     cGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTc                                                                                                                            >  2:1089678/1‑43 (MQ=255)                                                                                                     cGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTgg                                 >  2:596461/1‑134 (MQ=255)                                                                                                     cGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTgg                                 <  1:596461/134‑1 (MQ=255)                                                                                                     cGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTg                            <  2:837680/139‑1 (MQ=255)                                                                                                     cGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTg                            <  2:842369/139‑1 (MQ=255)                                                                                                                aTCTGGTTTCATCAACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTaa                 <  2:411847/139‑1 (MQ=255)                                                                                                                          atcaACAAAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAAt       >  1:890733/1‑139 (MQ=255)                                                                                                                               caaAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATcggcg  >  2:808284/1‑139 (MQ=255)                                                                                                                               caaAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATcggcg  <  1:68155/139‑1 (MQ=255)                                                                                                                               caaAGAAGGCACCCGTCCTGCGGTGGTTATCCCAACAAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATcggcg  <  2:508270/139‑1 (MQ=255)                                                                                                                                    aagGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGa                                                                         <  2:142874/63‑1 (MQ=255)                                                                                                                                    aagGCACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGa                                                                         >  1:142874/1‑63 (MQ=255)                                                                                                                                        |                                                                                                                                  TGGTGGTATCGGTGAAAATGCCGCAATGGTTCGTGAACTGTCTCTGGGCAAACTGGGCGTGCTGGGCTTTGAAGTTGATCATGAACGCAACCTGGCTGCACGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCG  >  NC_000913/2414462‑2414726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑