Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	410,683	A→G	K180K (AAA→AAG)	mak →	manno(fructo)kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	410,683	0	A	G	100.0%	79.7 / NA	22	K180K (AAA→AAG)	mak	manno(fructo)kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/12);  total (10/12)

GGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTG  >  NC_000913/410551‑410812                                                                                                                                     |                                                                                                                                  gggCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTGCCTTGTTATTGCGGTAAGCAAGGt                                                                                                                             >  2:619466/1‑139 (MQ=255)                tGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCttt                                                                                                              >  2:756529/1‑139 (MQ=255)                   gcgggcgCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTAtt                                                                                                           >  2:982444/1‑139 (MQ=255)                    cgggcgCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTAttt                                                                                                          <  1:615772/139‑1 (MQ=255)                            tatCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACg                                                                                                  <  1:622541/139‑1 (MQ=255)                                      aaTGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGa                                                                                        <  1:768790/139‑1 (MQ=255)                                            aCGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGCTTCGCGATGGAtt                                                                                  >  2:710788/1‑139 (MQ=255)                                                         ggggACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAg                                                                     <  2:874495/139‑1 (MQ=255)                                                         ggggACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAg                                                                     <  1:1033069/139‑1 (MQ=255)                                                          gggACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGa                                                                                        <  2:378494/119‑1 (MQ=255)                                                          gggACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGa                                                                                        >  1:378494/1‑119 (MQ=255)                                                          gggACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGc                                                                    >  2:728879/1‑139 (MQ=255)                                                              cacaATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGAc                                                                <  1:236644/139‑1 (MQ=255)                                                               acaATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACa                                                               <  1:619466/139‑1 (MQ=255)                                                                           cGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAgg                                                   >  1:393743/1‑139 (MQ=255)                                                                                        agacgaaCTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTcgc                                                                                          <  1:614743/87‑1 (MQ=255)                                                                                        agacgaaCTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTcgc                                                                                          >  2:614743/1‑87 (MQ=255)                                                                                                  cGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTg                            <  2:1079795/139‑1 (MQ=255)                                                                                                       tCGCGAGGAAGTCCCTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTgaagaa                       >  2:592897/1‑139 (MQ=255)                                                                                                                     cTTGTTATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGNACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGc         <  2:860721/139‑1 (MQ=255)                                                                                                                         ttATTGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGaa     <  1:1097593/139‑1 (MQ=255)                                                                                                                            ttGCGGTAAGCAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTg  >  1:930749/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                  GGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTG  >  NC_000913/410551‑410812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑