Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,001,154 T→C C270C (TGT→TGC)  xdhA → xanthine dehydrogenase, molybdenum binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,001,1540TC97.0% 109.2 / NA 33C270C (TGT→TGC) xdhAxanthine dehydrogenase, molybdenum binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (16/16);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGC  >  NC_000913/3001022‑3001270
                                                                                                                                    |                                                                                                                    
aCGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTc                                                                                                                <  2:1022483/139‑1 (MQ=255)
 cGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCg                                                                                                               <  2:945117/139‑1 (MQ=255)
               aTTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGc                                                                                                 >  1:65774/1‑139 (MQ=255)
                  tGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCACCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCAc                                                                                              <  1:18511/139‑1 (MQ=255)
                                                  tACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGaa                                                              >  1:145305/1‑139 (MQ=255)
                                                   aCTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAAc                                                             >  2:861451/1‑139 (MQ=255)
                                                   aCTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCATGAAc                                                             >  1:569694/1‑139 (MQ=255)
                                                      ggAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACcgc                                                          >  1:401944/1‑139 (MQ=255)
                                                          gagCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACg                                                      >  1:427380/1‑139 (MQ=255)
                                                            gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCg                                                                                                               <  2:849449/80‑1 (MQ=255)
                                                            gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCg                                                                                                               >  1:849449/1‑80 (MQ=255)
                                                            gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGAc                                                       <  1:343214/136‑1 (MQ=255)
                                                            gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGAc                                                       >  2:343214/1‑136 (MQ=255)
                                                            gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGa                                                    >  1:974958/1‑139 (MQ=255)
                                                            gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGa                                                    >  2:798858/1‑139 (MQ=255)
                                                            gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGa                                                    >  2:1022631/1‑139 (MQ=255)
                                                            gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGa                                                    >  1:472015/1‑139 (MQ=255)
                                                            gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGa                                                    >  2:962636/1‑139 (MQ=255)
                                                            gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGa                                                    <  2:974958/139‑1 (MQ=255)
                                                             ccAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGaa                                                   >  1:994117/1‑139 (MQ=255)
                                                             ccAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGaa                                                   <  2:65774/139‑1 (MQ=255)
                                                                    cggcATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGaa                                            <  2:638062/139‑1 (MQ=255)
                                                                    cggcATCCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTAAAGAGTGTTTCCTCGCACCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGaa                                            <  1:384881/139‑1 (MQ=255)
                                                                         ttCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGtt                                       <  2:16431/139‑1 (MQ=255)
                                                                          tCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTta                                      <  1:268252/139‑1 (MQ=255)
                                                                          tCCTGACCAGCAAGCTTGGCGACATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTta                                      <  2:653534/139‑1 (MQ=255)
                                                                                    cAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGAt                            >  2:576040/1‑139 (MQ=255)
                                                                                       gCTTGGCGGCATTCCGTTGAAAGTTTCCCTTAGCCGGGAAGAGTGCTTCCTCGCAACCCGAACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGtt                         <  2:458371/139‑1 (MQ=255)
                                                                                                     cGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCgg           <  2:411215/139‑1 (MQ=255)
                                                                                                        tGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGgcgc        <  2:427380/139‑1 (MQ=255)
                                                                                                            aGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTAt    <  2:472015/139‑1 (MQ=255)
                                                                                                            aGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGAAGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTAt    >  1:41637/1‑139 (MQ=255)
                                                                                                              tttCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGc  <  2:994117/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                    
ACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGC  >  NC_000913/3001022‑3001270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑