Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,005,344	A→G	L148P (CTT→CCT)	ygeV ←	putative sigma‑54‑interacting transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,005,344	0	A	G	100.0%	204.8 / NA	55	L148P (CTT→CCT)	ygeV	putative sigma‑54‑interacting transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/22);  total (33/22)

TTTTTAAGGCAGTCTGATTAACAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAAGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCAACACAACGATCCTGTAAA  >  NC_000913/3005213‑3005475                                                                                                                                    |                                                                                                                                    ttttgAAGGCAGTCTGATTAACAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTCACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGa                                                                                                                              <  2:346332/139‑1 (MQ=255)   tttAAGGCAGTCTGATTAACAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATTATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGaaa                                                                                                                            >  2:685344/1‑139 (MQ=255)            gTCTGATTAACAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAAtgg                                                                                                                   <  1:1109395/139‑1 (MQ=255)             tCTGATTAACAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAAtggt                                                                                                                  <  1:57890/139‑1 (MQ=255)             tCTGATTAACAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAAtggt                                                                                                                  >  2:78820/1‑139 (MQ=255)                     aCAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGatatat                                                                                                          >  2:690389/1‑139 (MQ=255)                       aaaCTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATgg                                                                                                        <  1:515217/139‑1 (MQ=255)                           tGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGttt                                                                                                                                 <  2:547780/110‑1 (MQ=255)                           tGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGttt                                                                                                                                 >  1:547780/1‑110 (MQ=255)                           tGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAAtgg                                                                                                                   >  2:1000913/1‑124 (MQ=255)                           tGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAAtgg                                                                                                                   <  1:1000913/124‑1 (MQ=255)                           tGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGaa                                                                                                    >  1:153204/1‑139 (MQ=255)                             aaCCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGCTCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACa                                                                                                  <  2:56116/139‑1 (MQ=255)                               cccGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATa                                                                                                >  1:536520/1‑139 (MQ=255)                                              cTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTcgcg                                                                                 <  1:690389/139‑1 (MQ=255)                                              cTACAACTAATACGCCCTCATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTcgcg                                                                                 >  1:618288/1‑139 (MQ=255)                                                   aCTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAAt                                                                            >  1:880442/1‑139 (MQ=255)                                                      aaTACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAGCAAAAATGGAGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTAt                                                                         <  1:685344/139‑1 (MQ=255)                                                       aTACGCCCTGATCCATATTATCGACCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCTTAAATTATc                                                                        >  1:750446/1‑139 (MQ=255)                                                        tACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCa                                                                       <  2:536520/139‑1 (MQ=255)                                                        tACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCa                                                                       >  1:246717/1‑139 (MQ=255)                                                        tACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCa                                                                       <  2:760179/139‑1 (MQ=255)                                                        tACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCa                                                                       >  2:168830/1‑139 (MQ=255)                                                             ccTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGGTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGat                                                                  >  1:740581/1‑139 (MQ=255)                                                              cTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGata                                                                 <  2:350419/139‑1 (MQ=255)                                                               tGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGatat                                                                >  2:307891/1‑139 (MQ=255)                                                                gATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATg                                                               <  2:939562/139‑1 (MQ=255)                                                                gATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATg                                                               <  2:695140/139‑1 (MQ=255)                                                                 aTCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGc                                                              >  2:128372/1‑139 (MQ=255)                                                                           tCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACa                                                                                                  >  2:603502/1‑93 (MQ=255)                                                                           tCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACa                                                                                                  <  1:603502/93‑1 (MQ=255)                                                                           tCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCt                                                    >  2:1067231/1‑139 (MQ=255)                                                                           tCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCt                                                    >  1:980969/1‑139 (MQ=255)                                                                           tCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTAGATATATGGCGAACATAATCAGAAAATTcgcg                                                                                 >  2:80186/1‑110 (MQ=255)                                                                           tCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTAGATATATGGCGAACATAATCAGAAAATTcgcg                                                                                 <  1:80186/110‑1 (MQ=255)                                                                                cATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTgg                                               >  2:498384/1‑139 (MQ=255)                                                                                cATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTgg                                               >  1:966528/1‑139 (MQ=255)                                                                                  tGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGt                                             <  2:510179/139‑1 (MQ=255)                                                                                        cAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGc                                       >  2:911006/1‑139 (MQ=255)                                                                                               tttACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCaaa                                >  2:225492/1‑139 (MQ=255)                                                                                                 tACTGATGTTATCTCCTGGCCCCTGATCCGCCAGAGGTTTCGAAAGAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACt                              >  1:641941/1‑139 (MQ=255)                                                                                                     gATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATc                          >  1:24815/1‑139 (MQ=255)                                                                                                     gATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATc                          >  2:415236/1‑139 (MQ=255)                                                                                                      aTGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTAt                           <  2:341630/137‑1 (MQ=255)                                                                                                      aTGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTAt                           >  1:341630/1‑137 (MQ=255)                                                                                                      aTGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCa                         >  1:290079/1‑139 (MQ=255)                                                                                                        gTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCAcc                       >  1:298868/1‑139 (MQ=255)                                                                                                           aTCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCa                    <  1:415236/139‑1 (MQ=255)                                                                                                            tctcCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCaa                   <  1:911006/139‑1 (MQ=255)                                                                                                                cTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCAAcaca               >  2:90965/1‑139 (MQ=255)                                                                                                                 tGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCAACACaa              <  2:366400/139‑1 (MQ=255)                                                                                                                  ggCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCAAACACAAc             >  1:1028497/1‑139 (MQ=255)                                                                                                                        tGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCAACACAACGATCCt       >  2:1105890/1‑139 (MQ=255)                                                                                                                            cctccAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCAACACAACGATCCTGTaa   <  1:307891/139‑1 (MQ=255)                                                                                                                             ctccAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCAACACAACGATCCTGTaaa  >  2:445144/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                    TTTTTAAGGCAGTCTGATTAACAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAAGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCAACACAACGATCCTGTAAA  >  NC_000913/3005213‑3005475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑