Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,035,437	T→C	K282R (AAG→AGG)	prfB ←	peptide chain release factor RF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,035,437	0	T	C	100.0%	131.0 / NA	35	K282R (AAG→AGG)	prfB	peptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/16);  total (19/16)

TAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTT‑CCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTC  >  NC_000913/3035305‑3035558                                                                                                                                      |                                                                                                                          tAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTT‑CCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCt                                                                                                                     >  1:192110/1‑139 (MQ=255) tAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTT‑CCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCt                                                                                                                     >  1:289237/1‑139 (MQ=255)  aaGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTT‑CCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTg                                                                                                                    >  2:1085513/1‑139 (MQ=255)       cGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGaa                                                                                                                >  2:483436/1‑139 (MQ=255)          aTCTGGCTTCCCCAGCCGATGTCGGATTTGTTATCTT‑CCATCGCCTGTTACTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGt                                                                                                            <  2:6843/139‑1 (MQ=255)          aTCTGGCTGCCCCAGCCGATGTCGGATTTGTTAACTT‑CCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGt                                                                                                            >  1:829405/1‑139 (MQ=255)                cTGCCCCAGCCGATGTCGGATTTGTTATCTT‑CCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCt                                                                                                      >  1:1034913/1‑139 (MQ=255)                    cccAGCCGATGTCGGATTTGTTATCTT‑CCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCa                                                                                                  <  1:747913/139‑1 (MQ=255)                                  gATTTGTTATCTT‑CCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATcc                                                                                    <  1:974375/139‑1 (MQ=255)                                             tt‑CCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGAtg                                                                         >  2:521563/1‑139 (MQ=255)                                                   tCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGt                                                                    >  1:479880/1‑139 (MQ=255)                                                         gTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACg                                                              <  2:829405/139‑1 (MQ=255)                                                          tttCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGc                                                             >  2:225296/1‑139 (MQ=255)                                                            tctcGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCAc                                                           <  2:354329/139‑1 (MQ=255)                                                              tcGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCg                                                         <  1:578237/139‑1 (MQ=255)                                                                ggCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCa                                                       <  2:549718/139‑1 (MQ=255)                                                                   aTTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGAt                                                    <  2:289237/139‑1 (MQ=255)                                                                              gCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGtt                                         <  1:1085513/139‑1 (MQ=255)                                                                               cATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGGTGTGGGTAATACGCACCGCAGATTCGGTACGGTTa                                        >  1:607664/1‑139 (MQ=255)                                                                               aaTCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGACTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCACAGATTCGTTACGGTTa                                        >  2:683895/2‑139 (MQ=255)                                                                                             aaaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccgcc                          <  1:483436/139‑1 (MQ=255)                                                                                                   tCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCa                                                                                                  >  1:475791/1‑61 (MQ=255)                                                                                                   tCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCa                                                                                                  <  2:475791/61‑1 (MQ=255)                                                                                                    cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCg                   >  2:146784/1‑139 (MQ=255)                                                                                                    cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCg                   >  1:232499/1‑139 (MQ=255)                                                                                                          cATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTg             <  1:521563/139‑1 (MQ=255)                                                                                                           aTCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGt                                                                                >  1:10591/1‑71 (MQ=255)                                                                                                           aTCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGt                                                                                <  2:10591/71‑1 (MQ=255)                                                                                                            tCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCg           <  1:146784/139‑1 (MQ=255)                                                                                                               gCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATa        <  2:474364/139‑1 (MQ=255)                                                                                                                cTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATaa       >  1:161106/1‑139 (MQ=255)                                                                                                                     tGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTc  >  2:51882/1‑139 (MQ=255)                                                                                                                     tGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTc  >  2:364171/1‑139 (MQ=255)                                                                                                                          tGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGAtgt                                                                        <  1:882875/64‑1 (MQ=255)                                                                                                                          tGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGAtgt                                                                        >  2:882875/1‑64 (MQ=255)                                                                                                                                      |                                                                                                                          TAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTT‑CCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTC  >  NC_000913/3035305‑3035558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑