Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,084,730	C→T	A394A (GCG→GCA)	speA ←	biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,084,730	0	C	T	100.0%	187.2 / NA	55	A394A (GCG→GCA)	speA	biosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/24);  total (31/24)

CCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTT  >  NC_000913/3084599‑3084864                                                                                                                                      |                                                                                                                                       ccGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCAtctt                                                                                                                                 >  2:1045128/1‑139 (MQ=255)         gTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGgc                                                                                                                         <  2:1090532/139‑1 (MQ=255)              cGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGt                                                                                                                    <  2:975452/139‑1 (MQ=255)                atatGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCg                                                                                                                  >  1:140612/1‑139 (MQ=255)                atatGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCg                                                                                                                  <  2:982990/139‑1 (MQ=255)                  atGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGc                                                                                                                >  1:187053/1‑139 (MQ=255)                   tGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCa                                                                                                               <  2:187053/139‑1 (MQ=255)                          cGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTa                                                                                                        >  1:573032/1‑139 (MQ=255)                           gTGCAGATCCATCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTAt                                                                                                       >  2:710254/1‑139 (MQ=255)                            tGCAGATCCATCTGACTGTCGTGTAACCATTCGCGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTAtt                                                                                                      >  2:739031/1‑139 (MQ=255)                            tGCAGATCCATCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTTCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTAtt                                                                                                      >  1:415712/1‑139 (MQ=255)                             gCAGATCCATCTGACTGTCGTGTAACCATTCACGCAGAGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTAt                                                                                                       <  2:329233/139‑1 (MQ=255)                                      tCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGTATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTc                                                                                            >  1:33525/1‑139 (MQ=255)                                      tCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGgc                                                                                                                         >  1:987250/1‑110 (MQ=255)                                      tCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGgc                                                                                                                         <  2:987250/110‑1 (MQ=255)                                      tCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTc                                                                                            >  2:262254/1‑139 (MQ=255)                                          aCTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACg                                                                                        <  1:631048/139‑1 (MQ=255)                                           cTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTGCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGc                                                                                       >  2:749830/1‑139 (MQ=255)                                            tGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGcc                                                                                      <  1:52003/139‑1 (MQ=255)                                              tCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCga                                                                                    <  1:1045128/139‑1 (MQ=255)                                              tCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCga                                                                                    <  1:874283/139‑1 (MQ=255)                                              tCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCga                                                                                    <  1:190664/139‑1 (MQ=255)                                                 tgtAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCgatga                                                                                 >  1:309196/1‑139 (MQ=255)                                                        aTTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCTAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCga                                                                                    <  1:148875/129‑1 (MQ=255)                                                        aTTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCTAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCga                                                                                    >  2:148875/1‑129 (MQ=255)                                                        aTTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGgcgc                                                                                                                       >  1:597001/1‑94 (MQ=255)                                                        aTTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGgcgc                                                                                                                       <  2:597001/94‑1 (MQ=255)                                                         ttCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTGTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGac                                                                         >  2:590891/1‑139 (MQ=255)                                                                           cGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGAt                                                         <  1:262254/139‑1 (MQ=255)                                                                              gTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCa                                                                                                               <  1:385185/82‑1 (MQ=255)                                                                              gTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCa                                                                                                               >  2:385185/1‑82 (MQ=255)                                                                              gTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATgcg                                                      >  2:505635/1‑139 (MQ=255)                                                                              gTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGAAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATgcg                                                      >  1:782920/1‑139 (MQ=255)                                                                               ttCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGTATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATgcgc                                                     <  2:33525/139‑1 (MQ=255)                                                                               ttCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATgcgc                                                     <  1:675615/139‑1 (MQ=255)                                                                               ttCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATgcgc                                                     <  2:177493/139‑1 (MQ=255)                                                                                tCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCa                                                    >  1:783352/1‑139 (MQ=255)                                                                                        cGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGc                                            >  2:1090374/1‑139 (MQ=255)                                                                                         gTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGgc                                                                                                                         >  2:443830/1‑61 (MQ=255)                                                                                         gTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGgc                                                                                                                         <  1:443830/61‑1 (MQ=255)                                                                                          tGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCAc                                          >  1:1065752/1‑139 (MQ=255)                                                                                          tGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCAc                                          >  2:208208/1‑139 (MQ=255)                                                                                              tctcCTGCCAGGTTTCCCACCTGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGAcc                                      <  1:21575/139‑1 (MQ=255)                                                                                                tcCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCg                                    <  1:590891/139‑1 (MQ=255)                                                                                                  cTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGAt                                  <  2:1065752/139‑1 (MQ=255)                                                                                                               cacaTGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCg                     <  2:573032/139‑1 (MQ=255)                                                                                                                   tGCTTTGCAGCGCGCGCGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCgg                 >  1:962853/1‑139 (MQ=255)                                                                                                                            gcgcgcgcGGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAg        >  1:213045/1‑139 (MQ=255)                                                                                                                             cgcgcgcgGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGa       >  1:992778/1‑139 (MQ=255)                                                                                                                             cgcgcgcgGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGa       >  1:978957/1‑139 (MQ=255)                                                                                                                             cgcgcgcgGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGa       <  1:768298/139‑1 (MQ=255)                                                                                                                             cgcgcgcgGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGa       >  2:849496/1‑139 (MQ=255)                                                                                                                             cgcgcgcgGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGa       >  1:501587/1‑139 (MQ=255)                                                                                                                               cgcgcgGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGAcc     >  2:767573/1‑139 (MQ=255)                                                                                                                               cgcgcgGTGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACG‑‑GTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGt   >  2:135678/1‑139 (MQ=255)                                                                                                                                  gcggtgCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGtt  <  1:749830/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       CCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTGTCGTGTAACCATTCACGC‑‑AGAGAACGGCGAGTTCCCGGTTCGTGCATCTCCTGCCAGGTTTCCCACATGCTTTGCAGCGCGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTT  >  NC_000913/3084599‑3084864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑