Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,276,481	G→A	S400S (TCG→TCA)	garD →	D‑galactarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,276,481	0	G	A	100.0%	189.3 / NA	54	S400S (TCG→TCA)	garD	D‑galactarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/25);  total (29/25)

AATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTA  >  NC_000913/3276350‑3276612                                                                                                                                    |                                                                                                                                    aaTATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTc                                                                                                                              >  1:221647/1‑139 (MQ=255)   tatGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTcca                                                                                                                            >  1:658180/1‑137 (MQ=255)     tGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACg                                                                                                                          >  1:379961/1‑139 (MQ=255)     tGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACg                                                                                                                          >  2:100241/1‑139 (MQ=255)     tGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACg                                                                                                                          >  2:116618/1‑139 (MQ=255)       ggAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGcc                                                                                                                        >  1:780718/1‑139 (MQ=255)       ggAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGcc                                                                                                                        >  2:553875/1‑139 (MQ=255)         aaaaaCCGACCGCAGCGCCAGCCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCGATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCg                                                                                                                      >  1:652929/1‑139 (MQ=255)         aaaaaCCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCg                                                                                                                      >  1:903320/1‑139 (MQ=255)               cgaccgCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGcc                                                                                                                        >  2:233446/1‑131 (MQ=255)               cgaccgCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGAAATTGTTGAAGTGCTGTCACCCGGTCAACGcc                                                                                                                        <  1:233446/131‑1 (MQ=255)                gaccgCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAAc                                                                                                               <  2:780718/139‑1 (MQ=255)                 accgCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAAcg                                                                                                              <  1:494854/139‑1 (MQ=255)                 accgCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAAcg                                                                                                              <  2:798497/139‑1 (MQ=255)                                   cGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTAcgccgc                                                                                            >  2:247125/1‑139 (MQ=255)                                        ggCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACgc                                                                                       >  1:127487/1‑139 (MQ=255)                                                    ggCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGccagccag                                                                                >  1:844844/1‑134 (MQ=255)                                                    ggCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGccagccag                                                                                <  2:844844/134‑1 (MQ=255)                                                     gCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGAttt                                                                          >  2:992784/1‑139 (MQ=255)                                                        gTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGt                                                                       <  2:964025/139‑1 (MQ=255)                                                                     ggTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAAcg                                                                                                              <  1:278007/87‑1 (MQ=255)                                                                     ggTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAAcg                                                                                                              >  2:278007/1‑87 (MQ=255)                                                                         gagaAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAgg                                                      <  2:652929/139‑1 (MQ=255)                                                                            aaGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTgg                                                   >  1:202455/1‑139 (MQ=255)                                                                              ggCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCt                                                 <  1:992784/139‑1 (MQ=255)                                                                              ggCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCt                                                 <  1:247125/139‑1 (MQ=255)                                                                               gCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCtt                                                >  1:580293/1‑139 (MQ=255)                                                                               gCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCtt                                                >  2:585676/1‑139 (MQ=255)                                                                               gCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCtt                                                >  2:1078891/1‑139 (MQ=255)                                                                                   tCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCggg                                            <  1:116618/139‑1 (MQ=255)                                                                                    cGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTAcgccg                                                                                             <  1:34728/89‑1 (MQ=255)                                                                                    cGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTAcgccg                                                                                             >  2:34728/1‑89 (MQ=255)                                                                                         ccATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATcac                                      >  2:992775/1‑139 (MQ=255)                                                                                            ttGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGt                                   >  2:849573/1‑139 (MQ=255)                                                                                               cTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACg                                                             <  2:1010542/110‑1 (MQ=255)                                                                                               cTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACg                                                             >  1:1010542/1‑110 (MQ=255)                                                                                                tAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCaa                               <  1:235352/139‑1 (MQ=255)                                                                                                    tCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAgtgt                           >  2:183299/1‑139 (MQ=255)                                                                                                       ggTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTa                        >  2:405574/1‑139 (MQ=255)                                                                                                       ggTAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTGTGCCTGTGGCACGCAACAGGAGGCTTCGGGTATCACAGTGCAAGAGTTTa                        >  1:1026069/1‑139 (MQ=255)                                                                                                         tAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGccagccag                                                                                >  1:864112/1‑81 (MQ=255)                                                                                                         tAAAAGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGccagccag                                                                                <  2:864112/81‑1 (MQ=255)                                                                                                           aaaGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGAc                    <  1:585676/139‑1 (MQ=255)                                                                                                           aaaGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGAc                    <  2:688151/139‑1 (MQ=255)                                                                                                           aaaGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGAc                    <  1:472990/139‑1 (MQ=255)                                                                                                           aaaGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGAc                    <  2:903320/139‑1 (MQ=255)                                                                                                            aaGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGAcc                   <  1:231622/139‑1 (MQ=255)                                                                                                            aaGCGCAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGAcc                   <  2:580293/139‑1 (MQ=255)                                                                                                              gcgcAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCgg                 <  2:364010/139‑1 (MQ=255)                                                                                                                 cAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACgc                                                                                       >  2:720007/1‑66 (MQ=255)                                                                                                                 cAATTGTTGAAGTGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACgc                                                                                       <  1:720007/66‑1 (MQ=255)                                                                                                                             tGCTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTa  <  1:1078891/139‑1 (MQ=255)                                                                                                                               cTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACgcca                                                                                     <  2:777341/54‑1 (MQ=255)                                                                                                                               cTGTCACCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACgcca                                                                                     >  1:777341/1‑54 (MQ=255)                                                                                                                                    |                                                                                                                                    AATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTA  >  NC_000913/3276350‑3276612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑