Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,505,331 T→C A230A (GCA→GCG)  yhfS ← FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,505,3310TC100.0% 134.7 / NA 36A230A (GCA→GCG) yhfSFNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/22);  total (14/22)

CAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGTGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGC  >  NC_000913/3505201‑3505462
                                                                                                                                  |                                                                                                                                   
cAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTg                                                                                                                             <  1:292276/139‑1 (MQ=255)
   tCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGAt                                                                                                                          <  2:246456/139‑1 (MQ=255)
        gcgcTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGc                                                                                                                     <  2:1015584/139‑1 (MQ=255)
         cgcTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCt                                                                                                                    <  1:1040115/139‑1 (MQ=255)
         cgcTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCt                                                                                                                    >  1:275156/1‑139 (MQ=255)
           cTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTAc                                                                                                                  <  2:348259/139‑1 (MQ=255)
           cTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTAc                                                                                                                  <  1:479812/139‑1 (MQ=255)
                        gcaccgcaccACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAg                                                                                                     >  2:947409/1‑139 (MQ=255)
                        gcaccgcaccACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAg                                                                                                     >  2:413723/1‑139 (MQ=255)
                        gcaccgcaccACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACAGCGTGCATCACTGGCGCAAACATCAGACCACGCAATACTTCCAGCGCATGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAg                                                                                                     >  1:547947/1‑139 (MQ=255)
                          atcgaaccgccGTTACGCAAAGCCAGCAACCATTAAGATACCCCTGTTCGTTCCGCGTGCATCACTGGCGTAAACCCCTGCCCTCGAAATCATTCCAGCGCCTGCGCGCACTTGATCTGGCTACCGCCGGAGTAAAGCg                                                                                                   <  1:1067348/134‑1 (MQ=255)
                              caccacCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGgc                                                                                               <  1:529372/139‑1 (MQ=255)
                                   ccGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGaa                                                                                          >  1:103118/1‑139 (MQ=255)
                                     gTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATa                                                                                        <  1:259866/139‑1 (MQ=255)
                                      ttAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATAc                                                                                       <  1:413723/139‑1 (MQ=255)
                                      ttAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATAc                                                                                       >  2:195702/1‑139 (MQ=255)
                                        aaGCAAAGCCAGCAACGGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACgg                                                                                     <  2:455034/139‑1 (MQ=255)
                                                     aaCCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAg                                                                        <  1:311510/139‑1 (MQ=255)
                                                                     ctgcctgcACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTAccgc                                                                                                               <  2:67852/84‑1 (MQ=255)
                                                                     ctgcctgcACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTAccgc                                                                                                               >  1:67852/1‑84 (MQ=255)
                                                                       gcctgcACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGaa                                                                                          >  1:502336/1‑103 (MQ=255)
                                                                       gcctgcACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGaa                                                                                          <  2:502336/103‑1 (MQ=255)
                                                                         ctgcACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCa                                                    <  1:306033/139‑1 (MQ=255)
                                                                                gcgTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCct                                             <  1:201559/139‑1 (MQ=255)
                                                                                 cgTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCctc                                            >  2:810489/1‑139 (MQ=255)
                                                                                    gCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTgg                                         <  1:611998/139‑1 (MQ=255)
                                                                                          cTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCaaa                                   <  1:1023119/139‑1 (MQ=255)
                                                                                                                        ccAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGAc                                                              <  1:253258/82‑1 (MQ=255)
                                                                                                                        ccAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGAc                                                              >  2:253258/1‑82 (MQ=255)
                                                                                                                         cAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTc    >  2:765245/1‑139 (MQ=255)
                                                                                                                           gcgcCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATa                                                                                        <  2:615299/53‑1 (MQ=255)
                                                                                                                           gcgcCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATa                                                                                        >  1:615299/1‑53 (MQ=255)
                                                                                                                           gcgcCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTcgc  >  1:321979/1‑139 (MQ=255)
                                                                                                                             gcCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATAc                                                                                       <  1:656708/52‑1 (MQ=255)
                                                                                                                             gcCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATAc                                                                                       >  2:656708/1‑52 (MQ=255)
                                                                                                                             gcCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGAt                                                                                <  2:463332/59‑1 (MQ=255)
                                                                                                                             gcCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGAt                                                                                >  1:463332/1‑59 (MQ=255)
                                                                                                                               cTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGAt                                                                                <  2:781589/57‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                   
CAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGTGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGC  >  NC_000913/3505201‑3505462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑